year 12, Issue 48 (12-2013)                   J. Med. Plants 2013, 12(48): 160-169 | Back to browse issues page

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Fallah Huseini H, Asghari B, Asgarpanah J, Babai Zarch A, Eghbali Zarch T. Effect of Polar and Nom-polar Aloe vera L. Leaf Extracts on α-amylase and α-Glucosidases Inhibitory Activity In vitro. J. Med. Plants 2013; 12 (48) :160-169
URL: http://jmp.ir/article-1-70-en.html
1- Pharmacology and Applied Medicine Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
2- Department of Plant Production and Breeding Engineering, Faculty of Engineering and Technology, Imam Khomeini International University
3- Department of Pharmacognosy, Pharmaceutical Science Branch, Islamic Azad University (IAU)
4- Department of Pharmacology Shaheed Seduqi Medical College Yazed
5- Department of Pharmacognosy, Pharmaceutical Science Branch, Islamic Azad University (IAU) , eghbali.tahereh@yahoo.com
Abstract:   (9426 Views)
Background: Intestinal α-glucosidase and α-amylase are two carbohydrate-hydrolysing enzymes that play a key role in conversion of disaccharide to glucose. Inhibition of these two enzymes provides a therapeutic option for treatment of postprandial hyperglycemia in diabetic patients. Objective: The aim of the current study was to evaluate the inhibitory effects of different Aloe vera L. extracts against α-amylase and α-glucosidase in vitro. Methods: Aloe vera L. leaf was collected from the Institute of Medicinal Plants farm and several extracts were prepared by n-hexane, chloroform, ethyl acetate and methanol solvents. The inhibitory effects of the extracts were tested on α-amylase and α-glucosidase enzyme separately. In this experiment, the minimum concentration of the extract required for 50% inhibition of enzyme activity (IC50), was obtained and compared with acarbose as a positive control. Results: The results showed that total methanol extract of Aloe vera L. leaf had significantly (p<0.001) higher α-amylase inhibitory (28.2 ± 1.3 µg/ml) effect than other solvent fractions but lower than acarbose (25.4 ± 1.9 µg/ml). Furthermore total methanol extract and solvent fractions of methanol and ethyl acetate of Aloe vera L. leaf had significantly (p<0.001) higher inhibitory effect on α-glucosidase enzyme activity (2.3 ± 0.1 µg/ml, 2.7 ± 0.1 µg/ml, 3.4 ± 0.1 µg/ml, respectively) than other solvent fractions as well as compared with acarbose (5.8 ± 0.6 µg/ml). Conclusion: Total methanol extracts of Aloe vera L. leaf has strong α-amylase and α-glucosidase inhibitory activity than chloroform, n-hexane, chloroform, ethyl acetate and methanol solvent fraction.
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Type of Study: Research | Subject: Pharmacognosy & Pharmaceutics
Received: 2013/06/18 | Accepted: 2013/08/7 | Published: 2013/12/21

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