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:: Volume 4, Number 48 (12-2013) ::
JMP 2013, 4(48): 160-169 Back to browse issues page
Effect of Polar and Nom-polar Aloe vera L. Leaf Extracts on α-amylase and α-Glucosidases Inhibitory Activity In vitro
H Fallah Huseini1, B Asghari2, J Asgarpanah3, A Babai Zarch4, T Eghbali Zarch *5
1- Ph.D. Pharmacology and Applied Medicine Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
2- Ph.D. Department of Plant Production and Breeding Engineering, Faculty of Engineering and Technology, Imam Khomeini International University
3- Ph.D. Department of Pharmacognosy, Pharmaceutical Science Branch, Islamic Azad University (IAU)
4- Ph.D. Department of Pharmacology Shaheed Seduqi Medical College Yazed
5- (Pharmacy student) Department of Pharmacognosy, Pharmaceutical Science Branch, Islamic Azad University (IAU) , eghbali.tahereh@yahoo.com
Abstract:   (3213 Views)
Background: Intestinal α-glucosidase and α-amylase are two carbohydrate-hydrolysing enzymes that play a key role in conversion of disaccharide to glucose. Inhibition of these two enzymes provides a therapeutic option for treatment of postprandial hyperglycemia in diabetic patients. Objective: The aim of the current study was to evaluate the inhibitory effects of different Aloe vera L. extracts against α-amylase and α-glucosidase in vitro. Methods: Aloe vera L. leaf was collected from the Institute of Medicinal Plants farm and several extracts were prepared by n-hexane, chloroform, ethyl acetate and methanol solvents. The inhibitory effects of the extracts were tested on α-amylase and α-glucosidase enzyme separately. In this experiment, the minimum concentration of the extract required for 50% inhibition of enzyme activity (IC50), was obtained and compared with acarbose as a positive control. Results: The results showed that total methanol extract of Aloe vera L. leaf had significantly (p<0.001) higher α-amylase inhibitory (28.2 ± 1.3 µg/ml) effect than other solvent fractions but lower than acarbose (25.4 ± 1.9 µg/ml). Furthermore total methanol extract and solvent fractions of methanol and ethyl acetate of Aloe vera L. leaf had significantly (p<0.001) higher inhibitory effect on α-glucosidase enzyme activity (2.3 ± 0.1 µg/ml, 2.7 ± 0.1 µg/ml, 3.4 ± 0.1 µg/ml, respectively) than other solvent fractions as well as compared with acarbose (5.8 ± 0.6 µg/ml). Conclusion: Total methanol extracts of Aloe vera L. leaf has strong α-amylase and α-glucosidase inhibitory activity than chloroform, n-hexane, chloroform, ethyl acetate and methanol solvent fraction.
Keywords: Aloe vera L., Diabetes, Enzyme inhibition, α-Glucosidase, α-Amylase
Full-Text [PDF 303 kb]   (791 Downloads)    
Type of Study: Research | Subject: Pharmacognosy
Received: 2014/09/30 | Accepted: 2014/09/30 | Published: 2014/09/30
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Fallah Huseini H, Asghari B, Asgarpanah J, Babai Zarch A, Eghbali Zarch T. Effect of Polar and Nom-polar Aloe vera L. Leaf Extracts on α-amylase and α-Glucosidases Inhibitory Activity In vitro. JMP. 2013; 4 (48) :160-169
URL: http://jmp.ir/article-1-70-en.html
Volume 4, Number 48 (12-2013) Back to browse issues page
فصلنامه علمی پژوهشی گیاهان دارویی Journal of Medicinal Plants
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