year 7, Issue 26 (5-2008)                   J. Med. Plants 2008, 7(26): 16-23 | Back to browse issues page

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Asghari G, Salimian Rizi T. The Influence of Fructose, Glucose and Sucrose on Flavonolignans Formation in Silybum marianum Callus Culture. J. Med. Plants 2008; 7 (26) :16-23
1- Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, IR Iran ,
2- Pharmacognosy Department, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, IR Iran
Abstract:   (6676 Views)
Background: Silybum marianum has been recognized as an antihepatotoxic plant. The active constituents of Silybum marianum include a group of flavonolignans known collectively as silymarin. Slymarin production by cultured cells of Silybum marianum has already been reported. Also, it was reported that physiochemical factors can influenced silymarin production in S. marinum cell cultures. Objective: Evaluation the affect of sugar source on flavonolignan production in S. marianum callus cultures. Method: Callus culture of S. marianum were established by transferring seedling on solidified MS medium supplemented with 1 mg/l 2, 4 dichlorophenoxy acetic acid and 0.2 mg/l kinetin. Optimal callus were subcultured to medium containing different concentration of sugars including fructose, glucose, and sucrose. Then cultures were harvested after 28 days, dried and extracted with methanol. Quantitative analyses of flavonolignans were carried out using spectrophotometric method. Results: Higher levels of flavonolignans accumulation were observed in cultures containing 6% of all three sugars comparing with concentrations of 3% and 1.5%. Conclusion: It seems flavonolignans production in callus culture of S. marianum influenced by concentration of sugars rather than of sugars type.
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Type of Study: Research | Subject: Biotechnology
Received: 2007/02/14 | Accepted: 2007/04/14 | Published: 2008/06/18

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