year 21, Issue 84 (12-2022)                   J. Med. Plants 2022, 21(84): 65-74 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Esmaeili H, Mirjalili M H, Zandi F. Antimicrobial and antioxidant activities of the leaf extract of some cultivated Iranian licorice populations. J. Med. Plants 2022; 21 (84) :65-74
URL: http://jmp.ir/article-1-3425-en.html
1- Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
2- Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran , m-mirjalili@sbu.ac.ir
3- Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
Abstract:   (1543 Views)
Background: Licorice (Glycyrrhiza glabra L.) is a well-known commercial medicinal plant with wide usage. Objective: During our ongoing aims for the selection and breeding programs of Iranian licorice (Glycyrrhiza glabra L.) populations, in the present study, total phenol content (TPC), total flavonoid content (TFC), and biological activities of the leaf extract of eight selected plant populations cultivated in the north of Tehran were investigated. Methods: The TPC and TFC were determined by Folin-Ciocalteu and aluminum chloride reagents, respectively. The antioxidant potential of the leaf extracts was measured through the evaluation of their power to reduce the Fe3+-TPTZ complex to Fe2+-TPTZ. The antibacterial activity was also assessed according to the broth micro-dilution method to determine minimum inhibitory concentration. Results: The results indicated that TPC varied from 6.18 ± 0.33 (mg GAE/g DW) in Bajgah population to 14.91 ± 1.17 (mg GAE/g DW) in Ilam population. The highest TFC was observed in Ilam (17.04 ± 1.25 mg rutin/g DW) and Marvest (15.06 ± 1.77 mg rutin/g DW) populations, respectively, without statistically significant differences. The maximum antioxidant activity was associated with the Ilam (532.18 ± 12.61 µmol Fe/g DW) population, confirming the antioxidant potential of phenolic and flavonoids compounds. The leaf extracts of Eghlid, Marvest, Ilam, and Bojnourd populations were exhibited outstanding antibacterial activity against S. aureus. Conclusion: The licorice leaf extracts showed more inhibitory effect against Staphylococcus aureus than Escherichia coli.
Full-Text [PDF 588 kb]   (1073 Downloads)    
Type of Study: Research | Subject: Pharmacognosy & Pharmaceutics
Received: 2022/09/9 | Accepted: 2022/11/1 | Published: 2022/12/1

References
1. Fauci AS, Touchette NA and Folkers GK. Emerging infectious diseases: a 10-year perspective from the National Institute of Allergy and Infectious Diseases. Emerg. Infect. Dis. 2005; 11(4): 519-525. [DOI:10.3201/eid1104.041167]
2. Akhtar N, Ihsan-ul-Haq and Mirza B. Phytochemical analysis and comprehensive evaluation of antimicrobial and antioxidant properties of 61 medicinal plant species. Arabian journal of chemistry. 2018; 11(8): 1223-1235. [DOI:10.1016/j.arabjc.2015.01.013]
3. Kaneria M, Baravalia Y, Vaghasiya Y and Chanda S. Determination of antibacterial and antioxidant potential of some medicinal plants from Saurashtra region, India. Indian J. Pharm. Sci. 2009; 71(4): 406-412. [DOI:10.4103/0250-474X.57289]
4. Voon HC, Bhat R and Rusul G. Flower extracts and their essential oils as potential antimicrobial agents for food uses and pharmaceutical applications. Comprehensive Reviews in Food Science and Food Safety 2011; 11(1): 34-55. [DOI:10.1111/j.1541-4337.2011.00169.x]
5. Stanković N, Mihajilov-Krstev T, Zlatković B, Stankov-Jovanović V, Mitić V, Jović J, Čomić L, Kocić B and Bernstein N. Antibacterial and antioxidant activity of traditional medicinal plants from the Balkan Peninsula. NJAS-Wageningen Journal of Life Sciences. 2016; 78(1): 21-28. [DOI:10.1016/j.njas.2015.12.006]
6. Rice-Evans CA, Sampson J, Bramley PM and Holloway DE. Why do we expect carotenoids to be antioxidants in vivo? Free radical research. 1997; 26(4): 381-398. [DOI:10.3109/10715769709097818]
7. Nassiri Asl M and Hosseinzadeh H. Review of pharmacological effects of Glycyrrhiza sp. and its bioactive compounds. Phytotherapy Res. 2008; 22 (6): 709-724. [DOI:10.1002/ptr.2362]
8. Celano R, Docimo T, Piccinelli AL, Rizzo S, Campone L, Sanzo RD, Carabetta S, Rastrelli L and Russo M. Specialized metabolite profiling of different Glycyrrhiza glabra organs by untargeted UHPLC-HRMS. Industrial Crops and Products 2021; 170: 113688. [DOI:10.1016/j.indcrop.2021.113688]
9. Hermann S, Orlik M, Boevink P, Stein E, Scherf A, Kleeberg I, Schmitt A and Schikora A. Biocontrol of plant diseases using Glycyrrhiza glabra leaf extract. Plant Disease 2022 (ja); 106(12): [DOI:10.1094/PDIS-12-21-2813-RE]
10. Siracusa L, Saija A, Cristani M, Cimino F, D'Arrigo M, Trombetta D, Rao F and Ruberto G. Phytocomplexes from liquorice (Glycyrrhiza glabra L.) leaves-Chemical characterization and evaluation of their antioxidant, anti-genotoxic and anti-inflammatory activity. Fitoterapia 2011; 82(4): 546-556. [DOI:10.1016/j.fitote.2011.01.009]
11. Esmaeili H, Karami A, Hadian J, Saharkhiz MJ and Nejad Ebrahimi SN. Variation in the phytochemical contents and antioxidant activity of Glycyrrhiza glabra populations collected in Iran. Industrial Crops and Products 2019; 137(1): 248-259. [DOI:10.1016/j.indcrop.2019.05.034]
12. Esmaeili H, Karami A, Hadian J, Nejad Ebrahimi S and Otto L-G. Genetic structure and variation in Iranian licorice (Glycyrrhiza glabra L.) populations based on morphological, phytochemical and simple sequence repeats markers. Industrial Crops and Products 2020; 145: 112140. [DOI:10.1016/j.indcrop.2020.112140]
13. Wahab S, Annadurai S, Abullais SS, Das G, Ahmad W, Ahmad MF, Kandasamy G, Vasudevan R, Ali MS and Amir M. Glycyrrhiza glabra (Licorice): A comprehensive review on its phytochemistry, biological activities, clinical evidence and toxicology. Plants 2021; 10(12): 2751-2787. [DOI:10.3390/plants10122751]
14. Jafari-Sales A and Bolouri P. Evaluation of the antimicrobial effects of Glycyrrhiza glabra L. on some gram positive and gram negative pathogenic bacteria in laboratory conditions. Jorjani Biomedicine Journal. 2018; 6(4): 78-84. [DOI:10.29252/jorjanibiomedj.6.4.78]
15. Karahan F, Avsar C, Ozyigit II and Berber I. Antimicrobial and antioxidant activities of medicinal plant Glycyrrhiza glabra var. glandulifera from different habitats. Biotechnology & Biotechnological Equipment. 2016; 30(4): 797-804. [DOI:10.1080/13102818.2016.1179590]
16. Iqbal Z, Hai Z, Ping HY, Ghaffar A, Mumtaz M and Liaqat L. Antioxidant and antibacterial activity of organic extracts of roots of Glycyrrhiza glabra Linn. Plant. 2017; 5(4): 68-72. [DOI:10.11648/j.plant.20170504.12]
17. Kamtekar S, Keer V and Patil V. Estimation of phenolic content, flavonoid content, antioxidant and alpha amylase inhibitory activity of marketed polyherbal formulation. Journal of Applied Pharmaceutical Science. 2014; 4(9): 061-065.
18. Zhishen J, Mengcheng T and Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999; 64(4): 555-559. [DOI:10.1016/S0308-8146(98)00102-2]
19. Benzie IFF and Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Analytical Biochem. 1996; 239 (1): 70-76. [DOI:10.1006/abio.1996.0292]
20. Jorgensen JH and Turnidge JD. Susceptibility test methods: dilution and disk diffusion methods. 11th Edition. Manual of Clinical Microbiology. 2015, 1253-1273. [DOI:10.1128/9781555817381.ch71]
21. Hayashi H, Hattori S, Inoue K, Khodzhimatov O, Ashurmetov O, Ito M and Honda G. Field survey of Glycyrrhiza plants in Central Asia (3). Chemical characterization of G. glabra collected in Uzbekistan. Chemical and pharmaceutical bulletin. 2003; 51(11): 1338-1340. [DOI:10.1248/cpb.51.1338]
22. Vlaisavljević S, Šibul F, Sinka I, Zupko I, Ocsovszki I and Jovanović-Šanta S. Chemical composition, antioxidant and anticancer activity of licorice from Fruska Gora locality. Industrial Crops and Products 2018; 112: 217-224. [DOI:10.1016/j.indcrop.2017.11.050]
23. Dong Y, Zhao M, Zhao T, Feng M, Chen H, Zhuang M and Lin L. Bioactive profiles, antioxidant activities, nitrite scavenging capacities and protective effects on H2O2-injured PC12 cells of Glycyrrhiza glabra L. leaf and root extracts. Molecules. 2014; 19(7): 9101-9113. [DOI:10.3390/molecules19079101]
24. Muflihah YM, Gollavelli G and Ling Y-C. Correlation study of antioxidant activity with phenolic and flavonoid compounds in 12 Indonesian indigenous herbs. Antioxidants. 2021; 10(10): 1530-1545. [DOI:10.3390/antiox10101530]
25. Li M, Pare PW, Zhang J, Kang T, Zhang Z, Yang D, Wang K and Xing H. Antioxidant capacity connection with phenolic and flavonoid content in Chinese medicinal herbs. Records of Natural Products. 2018; 12(3): 239-250. [DOI:10.25135/rnp.24.17.08.138]
26. Yordi EG, Pérez EM, Matos MJ and Uriarte E. Antioxidant and pro-oxidant effects of polyphenolic compounds and structure-activity relationship evidence. Nutrition, well-being and health. 2012; 2: 23-48.
27. Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P and Vidal N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem. 2006; 97(4): 654-660. [DOI:10.1016/j.foodchem.2005.04.028]
28. Ganeshpurkar A and Saluja AK. The pharmacological potential of rutin. Saudi Pharm. J. 2017; 25(2): 149-164. [DOI:10.1016/j.jsps.2016.04.025]
29. Frattaruolo L, Carullo G, Brindisi M, Mazzotta S, Bellissimo L, Rago V, Curcio R, Dolce V, Aiello F and Cappello AR. Antioxidant and anti-inflammatory activities of flavanones from Glycyrrhiza glabra L. (licorice) leaf phytocomplexes: Identification of licoflavanone as a modulator of NF-kB/MAPK pathway. Antioxidants. 2019; 8(6): 186. [DOI:10.3390/antiox8060186]
30. Irani M, Sarmadi M, Bernard F, Ebrahimi Pour GH and Shaker Bazarnov H. Leaves antimicrobial activity of Glycyrrhiza glabra L. IJPR. 2010; 9(4): 425-428.
31. Bassyouni R, Kamel Z, Megahid A and Samir E. Antimicrobial potential of licorice: Leaves versus roots. African Journal of Microbiology Research. 2012; 6(49): 7485-7493. [DOI:10.5897/AJMR12.1182]
32. Aggarwal H, Ghosh J, Rao A and Chhokar V. Evaluation of root and leaf extracts of Glycrriza glabra for antimicrobial activity. JOMB. 2015; 4(1): 81-85. [DOI:10.12720/jomb.4.1.81-85]
33. Rimando AM and Suh N. Biological/chemopreventive activity of stilbenes and their effect on colon cancer. Planta Med. 2008; 74(13): 1635-1643. [DOI:10.1055/s-0028-1088301]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Medicinal Plants

Designed & Developed by : Yektaweb