year 21, Issue 83 (9-2022)
J. Med. Plants 2022, 21(83): 72-86 |
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Tavakoli S, Khalighi-Sigaroodi F, Khosravi Dehaghi N, Yaghoobi M, Hajiaghaee R, Gholami A et al . Isolation and purification of apigenin, quercetin and apigenin 7-O-glycoside from Apium graveolens L., Petroselinum crispum (Mill.) Fuss, Allium cepa L., respectively. J. Med. Plants 2022; 21 (83) :72-86
URL: http://jmp.ir/article-1-3391-en.html
URL: http://jmp.ir/article-1-3391-en.html
Saeed Tavakoli1 
, Farahnaz Khalighi-Sigaroodi1 , Nafiseh Khosravi Dehaghi2 , Mahdi Yaghoobi3 , Reza Hajiaghaee1 , Ahmad Gholami4 , Reza Ghafarzadegan * 5
, Farahnaz Khalighi-Sigaroodi1 , Nafiseh Khosravi Dehaghi2 , Mahdi Yaghoobi3 , Reza Hajiaghaee1 , Ahmad Gholami4 , Reza Ghafarzadegan * 5
1- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
2- Evidence-Based Phytotherapy & Complementary Medicine Research Center, Alborz University of Medical Sciences, Karaj, Iran & Department of Pharmacognosy, School of Pharmacy, Alborz University of Medical Sciences, Karaj, Iran
3- Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
4- Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
5- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran , ghafarzadegan@imp.ac.ir
2- Evidence-Based Phytotherapy & Complementary Medicine Research Center, Alborz University of Medical Sciences, Karaj, Iran & Department of Pharmacognosy, School of Pharmacy, Alborz University of Medical Sciences, Karaj, Iran
3- Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
4- Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
5- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran , ghafarzadegan@imp.ac.ir
Abstract: (1740 Views)
Background: Flavonoids, a group of natural substances, are found in flowers, fruits, roots and stems. These natural products are well known for their health-beneficial effects. Hence, isolation and purifying of flavonoids from the ingredients is worthwhile. Flavonoids are now considered indispensable in various nutraceutical, pharmaceutical, medicinal and cosmetic applications. Apigenin, quercetin, and apigenin-7-O-glycoside have therapeutic properties that these two are in the flavonoid group. Objectives: In this study, apigenin, quercetin, and apigenin 7-O-glycoside were purified by column chromatography and identified by NMR and HPLC UV-visible techniques. Methods: Total extracts of three plants (Apium graveolens, Petroselinum crispum, Allium cepa) were divided into different solvent fractions. Column chromatography is used to purify these fractions. Finally, 1H-NMR, 13C-NMR, and mass spectrometry techniques were used for identification. Also, the HPLC technique was used for identifying these compounds and standardized. Results: In the present work, apigenin was extracted from ethyl acetate fraction of Apium graveolens seeds. High purity of apigenin 7-O-glycoside isolated from the butanol fraction of Petroselinum crispum aerial parts. Also, ethyl acetate fraction isolated quercetin from the Allium cepa. Identification of the data obtained from 1H-NMR and 13C-NMR, which were proofed by comparison with the sources of purified compounds. Conclusion: The methods used in this research were able to produce these three compounds with a high percentage of purity to be used in industry as standards.Background: Flavonoids, a group of natural substances, are found in flowers, fruits, roots and stems. These natural products are well known for their health-beneficial effects. Hence, isolation and purifying of flavonoids from the ingredients is worthwhile. Flavonoids are now considered indispensable in various nutraceutical, pharmaceutical, medicinal and cosmetic applications. Apigenin, quercetin, and apigenin-7-O-glycoside have therapeutic properties that these two are in the flavonoid group. Objectives: In this study, apigenin, quercetin, and apigenin 7-O-glycoside were purified by column chromatography and identified by NMR and HPLC UV-visible techniques. Methods: Total extracts of three plants (Apium graveolens, Petroselinum crispum, Allium cepa) were divided into different solvent fractions. Column chromatography is used to purify these fractions. Finally, 1H-NMR, 13C-NMR, and mass spectrometry techniques were used for identification. Also, the HPLC technique was used for identifying these compounds and standardized. Results: In the present work, apigenin was extracted from ethyl acetate fraction of Apium graveolens seeds. High purity of apigenin 7-O-glycoside isolated from the butanol fraction of Petroselinum crispum aerial parts. Also, ethyl acetate fraction isolated quercetin from the Allium cepa. Identification of the data obtained from 1H-NMR and 13C-NMR, which were proofed by comparison with the sources of purified compounds. Conclusion: The methods used in this research were able to produce these three compounds with a high percentage of purity to be used in industry as standards.
Keywords: Quercetin, Apigenin, Apigenin glycoside, Column chromatography, Apium graveolens, Petroselinum crispum, Allium cepa
Type of Study: Research |
Subject:
Medicinal Plants
Received: 2022/07/25 | Accepted: 2022/09/20 | Published: 2022/10/4
Received: 2022/07/25 | Accepted: 2022/09/20 | Published: 2022/10/4
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