year 21, Issue 83 (9-2022)                   J. Med. Plants 2022, 21(83): 87-98 | Back to browse issues page

Research code: A-12-1239-11
Ethics code: IR.ZUMS.REC.1399.415

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Ghassemifard L, Sardari S, Safari H, Ramezanikhah H, Khavasi N. Assessment of the antioxidant activity of the extrcat, cold press, and n-hexane oils and in vitro cytotoxic effects of Capparis spinosa L. seed on SH-SY5Y cancer cell lines. J. Med. Plants 2022; 21 (83) :87-98
1- Department of Persian Medicine, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
2- Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
3- Department of Persian Medicine, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran ,
Abstract:   (919 Views)
Background: Capparis spinosa L. due to its anti-inflammatory and antioxidant properties plays a key role in preventing and treatment of cancer, also reduces the growth rate of cancer cells. This plant can be used in various ways in medicinal compounds, food, etc. as an extract, oil, or essential oil. Objective: In the current study, the rate of antioxidant activity extracts and oils (cold press and n-hexane oils) of Capparis seeds and also the effect of the seeds on the SH-SY5Y cell line have been investigated. Methods: First, extract, cold press, and n-hexane oils were prepared; then all reached concentrations of 50, 100, and 200 mg/ml, and their antioxidant capacity was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. After that, the number of antioxidant compounds in the extract was measured, and finally, the toxicity of the extracts was evaluated by the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method on SH-SY5Y cancer cells. Results: The IC50 data showed that the antioxidant activity of the Capparis seeds extracts was significantly (P < 0.001) increased compared to cold press and n-hexane. Antioxidant compounds analysis of Capparis seeds extract indicated high rate of total phenolic, flavonoid as rutin, and quercetin. MTT assay demonstrates that Capparis seeds extracts in a concentration of 1000 μg/ml decreased the viability of the SH-SY5Y cancer cell lines in comparison with hacked cells (P < 0.001). Conclusion: The seed extract of Capparis seeds at high concentrations, probably due to its high antioxidant content, inhibits the growth of SH-SY5Y cancer cells.
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Type of Study: Research | Subject: Medicinal Plants
Received: 2022/06/12 | Accepted: 2022/09/4 | Published: 2022/10/8

1. Thompson M, Jaiswal Y, Wang I and Williams L. Hepatotoxicity: treatment, causes and applications of medicinal plants as therapeutic agents. J. Phytopharmacol. 2017; 6(3): 186-93: 1733-44. [DOI:10.31254/phyto.2017.6308]
2. AC, Mihai CT, Vochita G, Rotinberg P, Trifan A, Luca SV and et al. Antigenotoxic and antioxidant activities of a polyphenolic extract from European Dracocephalum moldavica L. Industrial Crops and Products. 2016; 79: 248-57. [DOI:10.1016/j.indcrop.2015.11.004]
3. Nabavi SF, Russo GL, Daglia M and Nabavi SMJFc. Role of quercetin as an alternative for obesity treatment: you are what you eat! 2015; 179: 305-10. [DOI:10.1016/j.foodchem.2015.02.006]
4. Russo M, Russo GL, Daglia M, Kasi PD, Ravi S, Nabavi SF and et al. Understanding genistein in cancer: The "good" and the "bad" effects: a review. 2016; 196: 589-600. [DOI:10.1016/j.foodchem.2015.09.085]
5. Khan MI, Bouyahya A, Hachlafi NEL, Menyiy NE, Akram M, Sultana S and et al. Anticancer properties of medicinal plants and their bioactive compounds against breast cancer: a review on recent investigations. Environ. Sci. Pollut. Res. Int. 2022; 29(17): 24411-44. [DOI:10.1007/s11356-021-17795-7]
6. Hall JC, Sytsma KJ, Iltis HH. Phylogeny of Capparaceae and Brassicaceae based on chloroplast sequence data. American Journal of Botany. 2002; 89(11): 1826-42. [DOI:10.3732/ajb.89.11.1826]
7. Afzal S, Afzal N, Awan MR, Khan TS, Khanum AG and Tariq S. Ethno-botanical studies from Northern Pakistan. J. Ayub. Med. Coll Abbottabad. 2009; 21(1): 52-7.
8. Ao M-z, Gao Y-y, Yu L-j. Advances in studies on constituents and their pharmacological activities of Capparis spinosa. Chinese Traditional and Herbal Drugs. 2007; 38(3): 463.
9. Mahboubi M and Mahboubi A. Antimicrobial activity of Capparis spinosa as its usages in traditional medicine. Herba Polonica. 2014; 60(1). [DOI:10.2478/hepo-2014-0004]
10. Mansour RB, Jilani IBH, Bouaziz M, Gargouri B, Elloumi N, Attia H and et al. Phenolic contents and antioxidant activity of ethanolic extract of Capparis spinosa. Cytotechnology. 2016; 68(1): 135-42. [DOI:10.1007/s10616-014-9764-6]
11. Tlili N, Elfalleh W, Saadaoui E, Khaldi A, Triki S and Nasri N. The caper (Capparis L.): Ethnopharmacology, phytochemical and pharmacological properties. Fitoterapia. 2011; 82(2): 93-101. [DOI:10.1016/j.fitote.2010.09.006]
12. Matthäus B and Özcan M. Glucosinolates and fatty acid, sterol, and tocopherol composition of seed oils from Capparis spinosa Var. spinosa and Capparis ovata Desf. Var. canescens (Coss.) Heywood. Journal of Agricultural and Food Chemistry. 2005; 53(18): 7136-41. [DOI:10.1021/jf051019u]
13. Ng T-B, Lam S-K, Cheung RC, Wong JH, Wang H-X, Ngai PH and et al. Therapeutic Use of Caper (Capparis spinosa) Seeds. Nuts and Seeds in Health and Disease Prevention: Elsevier; 2011, p. 279-84. [DOI:10.1016/B978-0-12-375688-6.10033-7]
14. Tlili N, El Guizani T, Nasri N, Khaldi A and Triki S. Protein, lipid, aliphatic and triterpenic alcohol content of caper seeds "Capparis spinosa". Journal of the American Oil Chemists' Society. 2011; 88(2): 265-70. [DOI:10.1007/s11746-010-1662-2]
15. Tlili N, MUNNE‐BOSCH S, Nasri N, Saadaoui E, Khaldi A and Triki S. Fatty acids, tocopherols and carotenoids from seeds of Tunisian caper "Capparis spinosa". Journal of Food Lipids 2009; 16(4): 452-64. [DOI:10.1111/j.1745-4522.2009.01158.x]
16. Matthaus B and Ozcan M. Glucosinolates and fatty acid, sterol, and tocopherol composition of seed oils from Capparis spinosa Var. spinosa and Capparis ovata Desf. Var. canescens (Coss.) Heywood. J. Agric. Food Chem. 2005; 53(18): 7136-41. [DOI:10.1021/jf051019u]
17. Kumar S and Pandey AK. Chemistry and biological activities of flavonoids: an overview. The Scientific World Journal. 2013; 2013. [DOI:10.1155/2013/162750]
18. Salvamani S, Gunasekaran B, Shaharuddin NA, Ahmad SA and Shukor MY. Antiartherosclerotic effects of plant flavonoids. BioMed Research International. 2014; 2014. [DOI:10.1155/2014/480258]
19. Xicoy H, Wieringa B and Martens GJ. The SH-SY5Y cell line in Parkinson's disease research: a systematic review. Mol. Neurodegener. 2017; 12(1): 10. [DOI:10.1186/s13024-017-0149-0]
20. Pizzi M, Boroni F, Bianchetti A, Moraitis C, Sarnico I, Benarese M and et al. Expression of functional NR1/NR2B-type NMDA receptors in neuronally differentiated SK-N-SH human cell line. Eur. J. Neurosci. 2002; 16(12): 2342-50. [DOI:10.1046/j.1460-9568.2002.02403.x]
21. Davidoff AM. Neuroblastoma. Seminars in Pediatric Surgery. 2012; 21(1): 2-14. [DOI:10.1053/j.sempedsurg.2011.10.009]
22. Mohagheghi SA, Poorazarang H, Elhamirad A, Dezashibi Z and Hematyar N. Extraction of phenolic compounds from potato peel (Ramus variety) with solvent and ultrasound-assisted methods and evaluation of its antioxidant activity in soybean oil. FSCT 2011; 8(28): 81-91.
23. Mohamed Ahmed IA, Özcan MM, AlJuhaimi F, Ghafoor K, Babiker EE, Osman MA and Alqah H. Quality characteristics of caper seed oils - The impact of extraction: Soxhlet versus cold pressing. Journal of Food Processing and Preservation 2021; 45(3): e15266. [DOI:10.1111/jfpp.15266]
24. Gardeli C, Vassiliki P, Athanasios M, Kibouris T and Komaitis MJFc. Essential oil composition of Pistacia lentiscus L. and Myrtus communis L.: evaluation of antioxidant capacity of methanolic extracts. Food Chem. 2008; 107(3): 1120-30. [DOI:10.1016/j.foodchem.2007.09.036]
25. Koparir P, Parlak AE, Karatepe A and Omar RA. Elucidation of potential anticancer, antioxidant and antimicrobial properties of some new triazole compounds bearing pyridine-4-yl moiety and cyclobutane ring. Arabian Journal of Chemistry. 2022: 103957. [DOI:10.1016/j.arabjc.2022.103957]
26. Justesen U and Knuthsen PJFc. Composition of flavonoids in fresh herbs and calculation of flavonoid intake by use of herbs in traditional Danish dishes. Food Chem. 2001; 73(2): 245-50. [DOI:10.1016/S0308-8146(01)00114-5]
27. Fahimi S, Abdollahi M, Mortazavi SA, Hajimehdipoor H, Abdolghaffari AH and Rezvanfar MAJIRCMJ. Wound healing activity of a traditionally used poly herbal product in a burn wound model in rats. Iran Red Crescent Med J. 2015; 17(9): [DOI:10.5812/ircmj.19960]
28. Patel V, Sharma V, Patidar AJIJoP and Sciences L. Quantitative analysis of rutin and quercetin in Capparis spinosa and Brassica oleracea by HPLC. IJPLS 2014; 5(8): 3720-24.
29. Lü L, Zhang L, Wai MSM, Yew DTW and Xu J. Exocytosis of MTT formazan could exacerbate cell injury. Toxicology in vitro. 2012; 26(4): 636-44. [DOI:10.1016/j.tiv.2012.02.006]
30. Roginsky V, Lissi EAJFc. Review of methods to determine chain-breaking antioxidant activity in food. 2005; 92(2): 235-54. [DOI:10.1016/j.foodchem.2004.08.004]
31. Özcan MM, Ahmed IAM, Al Juhaimi F, Uslu N, Osman MA, Gassem MA and et al. The influence of fermentation and bud sizes on antioxidant activity and bioactive compounds of three different size buds of Capparis ovata Desf. var. canescens plant. J Food Sci Technol. 2020; 57(7): 2705-12. [DOI:10.1007/s13197-020-04306-8]
32. Tlili N, Mejri H, Anouer F, Saadaoui E, Khaldi A, Nasri NJIC and et al. Phenolic profile and antioxidant activity of Capparis spinosa seeds harvested from different wild habitats. Ind. Crops Prod. 2015; 76: 930-5. [DOI:10.1016/j.indcrop.2015.07.040]
33. Khanavi M, Ara L, Khavasi N and Hajimehdipoor H. Capparis spinosa: a comparative study of raw and processed fruits. J. Med. Plants 2020; 19(73): 91-9. [DOI:10.29252/jmp.1.73.91]
34. Tir M, Feriani A, Labidi A, Mufti A, Saadaoui E, Nasri N and et al. Protective effects of phytochemicals of Capparis spinosa seeds with cisplatin and CCl4 toxicity in mice. Food Biosci. 2019; 28: 42-8. [DOI:10.1016/j.fbio.2019.01.002]
35. Yu L, Yang J, Wang X, Jiang B, Sun Y and Ji Y. Antioxidant and antitumor activities of Capparis spinosa L. and the related mechanisms. Oncology Reports. 2017; 37(1): 357-67. [DOI:10.3892/or.2016.5249]
36. Sheikh M, Eshraghi HR, Khoshnia M, Mazandarani M and Moradi A. Cytotoxic effect of Capparis spinosa L. on the PLC/PRF/5 human hepatocellular carcinoma cell line. Medical Laboratory Journal. 2017; 11(4): 9-12.
37. Kulisic-Bilusic T, Schmöller I, Schnäbele K, Siracusa L and Ruberto G. The anticarcinogenic potential of essential oil and aqueous infusion from caper (Capparis spinosa L.). Food chemistry. 2012; 132(1): 261-7. [DOI:10.1016/j.foodchem.2011.10.074]
38. Lam S-K and Ng T-B. A protein with antiproliferative, antifungal and HIV-1 reverse transcriptase inhibitory activities from caper (Capparis spinosa) seeds. Phytomedicine. 2009; 16(5): 444-50. [DOI:10.1016/j.phymed.2008.09.006]
39. Sheikh M, Eshraghi HR, Khoshnia M, Mazandarani M and Moradi A. Cytotoxicity effect of Capparis spinosa L. on the HepG2 human hepatocellular carcinoma cell line. Medical Laboratory Journal. 2017; 16(1): 73-7.

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