year 21, Issue 81 (3-2022)                   J. Med. Plants 2022, 21(81): 92-101 | Back to browse issues page

Research code: 50665

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sargazi Moghadam Z, Moshtaghi N, Sharifi A, Zahedi A, Khadem A. In vitro propagation and callus induction of medicinal endangered plant meadow rue (Thalictrum minus L.) for producing berberine. J. Med. Plants 2022; 21 (81) :92-101
1- Department of Agricultural Biotechnology, Ferdowsi University of Mashhad, Iran
2- Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran ,
3- Academic Center for Industrial Biotechnology, ACECR, Branch of Khorasan Razavi, Mashhad, Iran
Abstract:   (1966 Views)
Background: Thalictrum minus L. is a medicinal plant contains berberine, which is used for complementary treating of some diseases. Excessive harvesting of this species from rangelands to extract the alkaloid has endangered its survival. Objective: This study was aimed for in vitro propagation of this species and berberine production. Methods: The seeds were germinated at in vitro culture conditions. Then, the leaves were used as explants for callus induction in two MS and LS basal media supplemented with BA (0, 0.1, 0.5 and 1 mg/L) in combination with different concentrations of auxins, NAA, 2,4-D or IAA. Results: The results showed that MS medium with the plant growth regulator combination of 0.5 mg/L BA and 0.5 mg/L NAA had the highest callus formation and 100 % regeneration. Some calli were transferred to three liquid culture media (MS, LS, or ½ MS) with 1 mg/L BA + 0.1 mg/L NAA or without plant growth regulator to produce the berberine. The HPLC results identified 6 % of berberine in the liquid medium. This amount of berberine can be easily released into the culture medium, therefore, it will be easier to extract.  Conclusion: This method can be successfully used for propagation and extraction of berberine without plant destruction.
Full-Text [PDF 728 kb]   (649 Downloads)    
Type of Study: Research | Subject: Medicinal Plants
Received: 2021/11/24 | Accepted: 2022/02/22 | Published: 2022/03/1

1. Li Y, Kong D, Fu Y, Sussman M R and Wu H. The effect of developmental and environmental factors on secondary metabolites in medicinal plants. Plant Physiol. Biochem. 2020; 148: 80-89. [DOI:10.1016/j.plaphy.2020.01.006]
2. Ofoegbu A and Ettienne E B. Pharmacogenomics and morphine. ACCP. 2021; 61(9): 1149-1155. [DOI:10.1002/jcph.1873]
3. Idrees M, Hassan I U, Naeem M, Ali A, Aftab T, & Khan M M A. The Accumulation and Degradation of Alkaloids in Catharanthus roseus Supported by Various External Agents Under Different Environmental Conditions, in Catharanthus roseus. Springer Press. 2017; 321-329 , [DOI:10.1007/978-3-319-51620-2_13]
4. Garrepelly, J P, Kiranmayi, P, Bandari, T and Erva, R R. Molecular approach towards screening of biological targets of berberine and its production sources. Curr. Trends Biotechnol. Pharm. 2021; 15(2): 141-152,
5. Ahmed T, Abdollahi M, Daglia M, Nabavi S F and Nabavi S M. Berberine and neurodegeneration: A review of literature. Pharmacol. Rep. 2015; 67(5): 970-979, [DOI:10.1016/j.pharep.2015.03.002]
6. Brijwal L and Tamta S. Agrobacterium rhizogenes mediated hairy root induction in endangered Berberis aristata DC. Springer Plus. 2015; 4(1): 1, [DOI:10.1186/s40064-015-1222-1]
7. García-Gonzáles R, Quiroz K, Carrasco B, and Caligari P. Plant tissue culture: Current status, opportunities and challenges. IJANR. 2010; 37(3): 5-30. [DOI:10.4067/S0718-16202010000300001]
8. Lin L, He J, Lyu R., Yao M, Luo Y, and Xie L. The complete chloroplast genome of a distinctive meadow-rue, Thalictrum foeniculaceum (Ranunculaceae). Mitochondrial DNA Part B. 2021; 6(2): 404-405, [DOI:10.1080/23802359.2020.1870880]
9. Ikuta A and Itokawa H. Berberine and other protoberberine alkaloids in callus tissue of Thalictrum minus. Phytochemistry 1982; 21(6): 1419-1421, [DOI:10.1016/0031-9422(82)80154-4]
10. Hara M, Kobayashi Y, Fukui H, and Tabata M. Enhancement of berberine production by spermidine in Thalictrum-minus cell-suspension cultures. Plant Cell Rep. 1991; 10(10): 494-497, [DOI:10.1007/BF00234580]
11. Kobayashi Y, Fukui H and Tabata M. An immobilized cell culture system for berberine production by Thalictrum minus cells. Plant Cell Rep. 1987; 6(3): 185-186, [DOI:10.1007/BF00268474]
12. Dietrick E M. Embryo development, seed bank potential and germination of the federally endangered herb of pine savannas, Thalictrum. East Carolina University, Department of Biology .2016. Thesis.
13. Terasaka K, Sakai K, Sato F, Yamamoto H, and Yazaki K. Thalictrum minus cell cultures and ABC-like transporter. Phytochemistry 2003; 62(3): 483-489. [DOI:10.1016/S0031-9422(02)00548-4]
14. Bajpai V, Singh A, Kumar S, Sharma K R, and Kumar B. Determination of bioactive isoquinoline alkaloids in Thalictrum reniforme Wallich and Thalictrum neurocarpum Royale using ultra performance liquid chromatography with hybrid triple quadrupole linear ion trap mass spectrometer. J. Med. Plants Stud. 2017; 5(3): 234-240.
15. Walck J L, Baskin C C and Baskin J M. Seeds of Thalictrum mirabile (Ranunculaceae) require cold stratification for loss of non-deep simple morphophysiological dormancy. Can. J. Bot. 1999; 77: 1769-1776, [DOI:10.1139/cjb-77-12-1769]
16. Lee SY, Rhie Y H and Kim K S. Dormancy breaking and germination requirements of seeds of Thalictrum uchiyamae (Ranunculaceae) with underdeveloped embryos. Sci. Hortic. 2018; 231: 82-88, [DOI:10.1016/j.scienta.2017.12.004]
17. Hara M, Tanaka S and Tabata M. Induction of a specific methyltransferase activity regulating berberine biosynthesis by cytokinin in Thalictrum minus cell cultures. Phytochemistry 1994; 36(2):327-332, [DOI:10.1016/S0031-9422(00)97070-5]
18. Nakagawa K, Konagai A, Fukui H and Tabata M. Release and crystallization of berberine in the liquid medium of Thalictrum minus cell suspension cultures. Plant Cell Rep. 1984; 3(6): 254-257, [DOI:10.1007/BF00269306]
19. Nakagawa K, Fukui H and Tabata M. Hormonal regulation of berberine production in cell suspension cultures of Thalictrum minus. Plant Cell Rep. 1986; 5(1):69-71, [DOI:10.1007/BF00269722]
20. Mishra M K, Pandey S, Misra P, Niranjan A, and Srivastava A. An efficient protocol for clonal regeneration and excised root culture with enhanced alkaloid content in Thalictrum foliolosum DC.-an endemic and important medicinal plant of temperate Himalayan region. Ind. Crops Prod. 2020; 152: 112504. [DOI:10.1016/j.indcrop.2020.112504]
21. Jan M, Singh S, Maqbool F and Nawchoo I A. Direct shoot regeneration from hypocotyl explants of Heracleum candicans Wall: A vulnerable high value medicinal herb of Kashmir Himalaya. Afr. J. Agric. Res. 2018; 13(28):1419-1424, [DOI:10.5897/AJAR2017.12726]
22. Phulwaria M, Shekhawat N, Rathore J and Singh R. An efficient in vitro regeneration and ex vitro rooting of Ceropegia bulbosa Roxb.-a threatened and pharmaceutical important plant of Indian Thar Desert. Ind. Crops Prod. 2013; 42:25-29, [DOI:10.1016/j.indcrop.2012.05.013]
23. Kulkarni A A, Thengane S and Krishnamurthy K. Direct shoot regeneration from node, internode, hypocotyl and embryo explants of Withania somnifera. PCTOC. 2000; 62(3): 203-209,
24. Shrivastava N and Rajani M. Multiple shoot regeneration and tissue culture studies on Bacopa monnieri (L.) Pennell. Plant Cell Rep. 1999; 18(11): 919-923, [DOI:10.1007/s002990050684]
25. Yamamoto H, Nakagawa K, Fukui H and Tabata M. Cytological changes associated with alkaloid production in cultured cells of Coptis japonica and Thalictrum minus. Plant Cell Rep. 1986; 5(1): 65-68, [DOI:10.1007/BF00269721]
26. Shitan N, Terasaka K, Yamamoto H, Sato F, and Yazaki K. Two B-type ATP-binding cassette (ABC) transporters localize to the plasma membrane in Thalictrum minus. Plant Biotechnology. 2015; 32(3): 243-247, [DOI:10.5511/plantbiotechnology.15.0604a]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Medicinal Plants

Designed & Developed by : Yektaweb