year 21, Issue 81 (3-2022)                   J. Med. Plants 2022, 21(81): 33-50 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Al-Baidhani R, Rezadoost H, Hamidi A, Motevali S M, Mirzajani F. The α-amylase and α-glucosidase inhibitory effects of some traditional antidiabetic prescriptions based on bioautography using LC-ESI/MSMS. J. Med. Plants. 2022; 21 (81) :33-50
1- Protein Research Center, Shahid Beheshti University, G.C., Tehran, Iran
2- Department of Phytochemistry, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, G.C., Tehran, Iran
3- School of Chemistry, University of New South Wales, Sydney, NSW, 2052 Australia
4- Protein Research Center, Shahid Beheshti University, G.C., Tehran, Iran ,
Abstract:   (571 Views)
Background: Diabetes, one of the most common metabolic diseases in many societies, has influenced the quality of human life for many years. Traditional, alternative, and complementary medicine use individual or mixed plant prescriptions to reduce adverse consequences of diabetes. This is based on the human experience of treating and managing disease complications in different geographic areas, over a hundred years. Objective: This research tries to find and recommend the most influential traditional medicine prescription for the inhibition of key enzymes associated with diabetes. Methods: Considering the full and widespread use of the medicinal plants, in this research, 15 most reliable Iranian and Iraqi herbal prescriptions in controlling diabetes were studied for the inhibition of α-amylase and α-glucosidase enzymes. Chemicals of the most effective prescription for the inhibition of these enzymes were separated by using the HPTLC method. For chromatogram development, a mobile phase consisting of ethyl acetate: toluene: methanol: formic acid was used. A direct enzyme inhibitory assay on the HPTLC plate was used to investigate the most effective molecules. Thereafter, the influential molecules were identified by using LC-ESI/MSMS. Results: Based on the results, prescription No. 3, (from the Iraqi herbal prescription) containing the extract of Prunus mahaleb L. and Prunus dulcis (Mill.) D.A.Webb was identified as the best α-amylase and α-glucosidase inhibitor. Conclusion: The chemical and molecular analysis of this extract which was performed by the HPTLC method and further by LC-ESI/MSMS, indicates two compounds of catechin and epicatechin.
Full-Text [PDF 1274 kb]   (271 Downloads)    
Type of Study: Research | Subject: Medicinal Plants
Received: 2021/11/7 | Accepted: 2022/01/22 | Published: 2022/03/1

1. Zhao P, Zhao C, Li X, Gao Q, Huang L, Xiao P and Gao W. The genus Polygonatum: A review of ethnopharmacology, phytochemistry and pharmacology. Journal of Ethnopharmacology. 2018; 214 (April 2017): 274-291. doi: 10.1016/j.jep. 2017.12.006.
2. Aghanouri Z, Siavash M, Mombeini H, Monfared M, Mojahedi M and Ilkhani R. Extended honeymoon period in a type 1 diabetic child by Iranian Traditional Medicine treatments, a case report. Primary Care Diabetes. 2017; 11(6): 583-585. doi: 10.1016/j.pcd.2017.08.001.
3. Cho N, Kirigia J, Ogurstova K and Reja A. IDF Diabetes Atlas (Internet).; 2017.
4. Ala AO, Ojo OA, Enikuomehin CA, Ajani GO, Olamoyegun MA, Akinlade AT and Olabode OR. Prevalence and Determinants of Complementary and Alternative Medicine (CAM) Use among Diabetes Patients in Southwestern Nigeria. West African Journal of Medicine. 2020; 37(5): 528-536.
5. Devi K, Santhini E, Manikandan R and Prabhu NM. The prevalence, awareness and potential of complementary alternative medicine in type 2 diabetics living in Madurai, India. European Journal of Integrative Medicine. 2015; 7(5): 469-473. doi:10.1016/j.eujim.2015.04.003.
6. Asghari B, Salehi P, Sonboli A and Ebrahimi SN. Flavonoids from Salvia chloroleuca with α-amylsae and α-glucosidase inhibitory effect. Iranian Journal of Pharmaceutical Research. 2015; 14(2): 609-615. doi: 10.22037/ijpr.2015. 1651.
7. Asghari B, Salehi P, Farimani MM and Ebrahimi SN. α-Glucosidase Inhibitors from Fruits of Rosa canina L. Records of Natural Products. 2015; 9 (3): 276-283.
8. Özcan K, Acet T and Çorbacı C. Centaurea hypoleuca DC: Phenolic content, antimicrobial, antioxidant and enzyme inhibitory activities. South African Journal of Botany. 2019; 127: 313-318. doi: 10.1016/j.sajb.2019.10.020.
9. Shimabukuro M, Higa M, Yamakawa K, Masuzaki H and Sata M. Miglitol, α-glycosidase inhibitor, reduces visceral fat accumulation and cardiovascular risk factors in subjects with the metabolic syndrome: A randomized comparable study. International Journal of Cardiology. 2013; 167(5): 2108-2113. doi: 10.1016/j.ijcard. 2012.05. 109.
10. Jichen Y, Xiaoli W, Chuanying Z, Lun M, Tao W, Yajing Z and Xin P. Comparative study of inhibition mechanisms of structurally different flavonoid compounds on α-glucosidase and synergistic effect with acarbose. Food Chem. 2021; 347: 129056. doi: 10.1016/J. FOODCHEM.2021.129056.
11. Sharif I, Yarash T, Masood F, Clifford RM, Davis W and Davis TME. Complementary and alternative medicine beliefs in type 2 diabetes: The Fremantle Diabetes Study Phase II. Diabetes Research and Clinical Practice. 2020; 166: 108311. doi: 10.1016/j.diabres.2020. 108311.
12. Yeung AWK, Heinrich M, Kijjoa A, Tzvetkov NT and Atanasov AG. The ethnopharmacological literature: An analysis of the scientific landscape. Journal of Ethnopharmacology. 2020; 250: 112414. doi: 10.1016/j.jep.2019.112414.
13. Yue H, Zeng H and Ding K. A review of isolation methods, structure features and bioactivities of polysaccharides from Dendrobium species. Chinese Journal of Natural Medicines. 2020; 18(1): 1-27. doi: 10.1016/S1875-5364(20)30001-7.
14. Shari FH, Ramadhan HH, Mohammed RN and Al-Bahadily DC. Hypolipidemic and antioxidant effects of fenugreek- nigella sativa combination on diabetic patients in Iraq. Systematic Reviews in Pharmacy. 2020; 11(6): 911-915. doi: 10.31838/ srp.2020.6.128.
15. Akbarzadeh T, Sabourian R, Saeedi M, Rezaeizadeh H, Khanavi M and Ardekani MRS. Liver tonics: Review of plants used in Iranian traditional medicine. Asian Pacific Journal of Tropical Biomedicine. 2015; 5(3): 170-181. doi: 10.1016/S2221-1691(15)30002-2.
16. Asadi-Samani M, Kafash-Farkhad N, Azimi N, Fasihi A, Alinia-Ahandani E and Rafieian-Kopaei M. Medicinal plants with hepatoprotective activity in Iranian folk medicine. Asian Pacific Journal of Tropical Biomedicine. 2015; 5(2): 146-157. doi: 10.1016/S2221-1691(15)30159-3.
17. Wang M, Zhang Y, Wang R, Wang Z, Yang B and Kuang H. An evolving technology that integrates classical methods with continuous technological developments: Thin-layer chromatography bioautography. Molecules. 2021; 26(15). doi: 10. 3390/molecules26154647.
18. Glucosidase and -amylase inhibitory effect and antioxidant activity of ten plant extracts traditionally used in Iran for diabetes. Journal of Medicinal Plants Research. 2013; 7(6): 257-266. doi: 10.5897/JMPR11.1320.
19. Wu X, Hu M, Hu X, Ding H, Gong D and Zhang G. Inhibitory mechanism of epicatechin gallate on α-amylase and α-glucosidase and its combinational effect with acarbose or epigallocatechin gallate. Journal of Molecular Liquids. 2019; 290. doi: 10.1016/j.molliq.2019. 111202.
20. Brayer GD, Sidhu G, Maurus R, Rydberg EH, Braun C, Wang Y, Nguyen NT, Overall CM and Withers SG. Subsite mapping of the human pancreatic α-amylase active site through structural, kinetic, and mutagenesis techniques. Biochemistry. 2000; 39(16): 4778-4791. doi: 10.1021/bi9921182.
21. Grosdidier A, Zoete V and Michielin O. SwissDock, a protein-small molecule docking web service based on EADock DSS. Nucleic Acids Research. 2011; 39(SUPPL. 2). doi: 10.1093/nar/ gkr366.
22. Grosdidier A, Zoete V and Michielin O. EADock: Docking of small molecules into protein active sites with a multiobjective evolutionary optimization. Proteins: Structure, Function and Genetics. 2007; 67(4): 1010-1025. doi: 10.1002/prot.21367.
23. Pettersen EF, Goddard TD, Huang CC, Couch GS, Greenblatt DM, Meng EC and Ferrin TE. UCSF Chimera - A visualization system for exploratory research and analysis. Journal of Computational Chemistry. 2004; 25(13): 1605-1612. doi: 10.1002/jcc.20084.
24. Lalegani S, Ahmadi Gavlighi H, Azizi MH and Amini Sarteshnizi R. Inhibitory activity of phenolic-rich pistachio green hull extract-enriched pasta on key type 2 diabetes relevant enzymes and glycemic index. Food Research International. 2018; 105: 94-101. doi: 10.1016/ j.foodres.2017.11.003.
25. Yilmazer-Musa M, Griffith AM, Michels AJ, Schneider E and Frei B. Inhibition of α-amylase and α-glucosidase activity by tea and grape seed extracts and their constituent catechins. Journal of Agricultural and Food Chemistry. doi: 10.1021/jf301147n.
26. Yilmazer-Musa M, Griffith AM, Michels AJ, Schneider E and Frei B. Grape seed and tea extracts and catechin 3-gallates are potent inhibitors of α-amylase and α-glucosidase activity. In: Journal of Agricultural and Food Chemistry. Vol 60. NIH Public Access; 2012: 8924-8929. doi: 10.1021/jf301147n.

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Journal of Medicinal Plants

Designed & Developed by : Yektaweb