year 20, Issue 79 (8-2021)                   J. Med. Plants 2021, 20(79): 24-36 | Back to browse issues page

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Valizadeh G, Nejad Ebrahimi S, Gholami M, Mazaheritehrani M, Rezadoost H, Sonboli A. Rearranged abietane diterpenoids from roots of Teucrium hircanicum L.. J. Med. Plants 2021; 20 (79) :24-36
1- Department of Chemistry, Faculty of Basic Sciences, Golestan University, Gorgan, Iran
2- Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran ,
3- Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran
4- Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran
Abstract:   (1893 Views)
Background: Medicinal plants play a significant role in preventing and treating diseases due to their traditional uses. Objective: The aim of the study was to isolate, purify and identify phytochemicals from the n-hexane extract of Teucrium hircanicum L. roots and evaluate the antioxidant activity of the extract and its purified compounds. Methods: The n-hexane extract (2.27 g) was extracted from the roots of this plant. The extract was fractionated by column chromatography with normal phase by eluting with n-hexane-EtOAc and following with EtOAc-Methanol. Fractions with similar phytochemical fingerprints combined to produce 23 main fractions. Final purification was carried out by preparative reversed-phase HPLC-UV. The structures of isolated were secured by different spectroscopic methods such as 1D, 2D NMR, and mass spectroscopy methods and comparing of these data with literature reported values. The antioxidant activity of the n-hexane extract of T. hircanicum roots and its purified diterpenoids was evaluated in DPPH assay and radical scavenging activity was calculated. Results: Extraction and isolation methods were used to purify three rearranged abietane-type diterpenoids villosin A (1), teuvincenone B (2) and 5, 8, 11, 13, 15-abietapentaen-7-one (3), a phenethyl ester namely 4-hydroxyphenethyl pentacosanoate (4) and one sterol namely 22-dehydroclerosterol (5). Conclusion: The n-hexane extract from roots of T. hircanicum and three rearranged abietane type diterpenoids were showed good antioxidant activities ranged from 3.5-4.3 μg/ml compared with the reference value (BHT = 16.5 μg/ml).
Full-Text [PDF 593 kb]   (924 Downloads)    
Type of Study: Research | Subject: Pharmacognosy & Pharmaceutics
Received: 2021/03/25 | Accepted: 2021/06/27 | Published: 2021/09/1

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