year 18, Issue 72 And S12 (Supplement 12 2019)                   J. Med. Plants 2019, 18(72 And S12): 90-102 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

shaghaghi J, kordenaeej A, qaderi A. Evaluation of Genetic Diversity of Papaver bractaetum Lindl. Populations in Alborz Mountain Using SCoT Molecular Markers. J. Med. Plants 2019; 18 (72) :90-102
URL: http://jmp.ir/article-1-2553-en.html
1- Department of Agricultural and Plant Breeding, Faculty of Agriculture, Shahed University, Tehran, Iran , javad_shaghaghi@yahoo.com
2- Department of Agricultural and Plant Breeding, Faculty of Agriculture, Shahed University, Tehran, Iran
3- Medicinal Plant Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
Abstract:   (3007 Views)
Background: Iranin poppy (Papaver bracteatum Lindl.) belongs to Papaveraceae family which has important alkaloids such as thebain.
Objective: Investigation of genetic diversity and population structure of Iranian poppy populations from Alborz Mountains.
Methods: Total DNA from 36 genotypes (12 individuals from each population) were extracted by doyle method. Thebaine of capsules were assayed by High performance liquid chromatography (HPLC) against standard curve of thebaine.
Results: The 12 primers from 26applied SCoT primers amplified 181 scorable bands. Polymorphism Information Contents (PIC) index ranged from 0.233 to 0.335 with 0.289 mean. Resolving Power (RP) index ranged from 2.61 to 6.5. based on AMOVA the variance of within and among population were 57% and 43% respectively. Unweighted pair group method with arithmetic mean (UPGMA) based clustering grouped 36 genotypes into three main groups that was supported by principal coordinate analyses (PCoA). The highest (0.86) and lowest (0.76) similarity were between Polur, Siahbisheh and Taham, Siahbisheh populations respectively. Also, thebaine content was differences between populations. The maximum and minimum thebaine content was obtained in Polur and Taham population respectively.
Conclusion: The results showed that the SCoT markers can separate the Iranian poppy population and it’s an efficient tool for study of genetic diversity and population structure. Also results clarified considerable genetic diversity among P. bracteatum population in Alboraz mountains. Based on results of phytochemical and genetic study, thebaine production in Iranian poppy is controled by genetic and environment factors.
Full-Text [PDF 892 kb]   (1173 Downloads)    
Type of Study: Research | Subject: Biotechnology
Received: 2019/06/17 | Accepted: 2019/11/27 | Published: 2020/03/7

References
1. Amiryousefi A, Hyvonen J and Poczai P. iMEC: Online Marker Efficiency Calculator. Applications in Plant Sciences 2018; 6 (6): e1159. [DOI:10.1002/aps3.1159]
2. Anderson JA, Churchill GA, Autrique JE, Tanksley SD and Sorrells ME. Optimizing parental selection for genetic linkage maps. Genome 1993; 36: 181 - 186. [DOI:10.1139/g93-024]
3. Antonio AFG, Benchimol LL, Barbosa AMM, Geraldi IO and Souza CL. Comparison of RAPD, RFLP, AFLP, SSR marker for diversity studies in tropical maize inbred lines. Gen. Mol. Bio. 2004; 27: 579 - 88. [DOI:10.1590/S1415-47572004000400019]
4. Bhattacharyya P, Kumaria S, Kumar S and Tandon P. Start codon targeted (SCoT) Marker reveals genetic diversity of Dendrobium nobile Lindl. An endangered medicinal Orchid species. Geneme 2013; 529: 21-26. [DOI:10.1016/j.gene.2013.07.096]
5. Bhawna AMZ, Arya L and Verma M. Use of SCoT markers to assess the gene flow and population structure among two different populations of bottle gourd. Plant Gene. 2017; 9: 80-86. [DOI:10.1016/j.plgene.2016.09.001]
6. Collard BCY and Mackill DJ. Start codon targeted (SCoT) polymorphism: a simple, Novel DNA marker technique for generating gene targeted markers in plants. Plant Mol. Biol. 2009; 27: 86-93. [DOI:10.1007/s11105-008-0060-5]
7. Doyle JJ and Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 1987; 19: 11-15.
8. Evanno G, Regnaut S and Goudet J. Detecting the Number of Clusters of Individuals Using the Software STRUCTURE: A Simulation Study. Mol. Ecol. 2005; 14: 2611-2620. [DOI:10.1111/j.1365-294X.2005.02553.x]
9. Farsi M and Zolali J. Principle of Plant biotechnology. Ferdosi Mashhad University Press 2004, pp: 495.
10. Fiehn O. Metabolomics the link between genotypes and phenotypes. Plant Mol. Biol. 2002; 48: 155 - 171. [DOI:10.1007/978-94-010-0448-0_11]
11. Goldblatt P. Biosystematic studies in Papaver section Oxytona. Annals of the Missouri Botanical Garden. 1974; 61: 264 - 296. [DOI:10.2307/2395056]
12. Gorji AM, Poczai P, Polgar Z and Taller J. Efficiency of arbitrarily amplified dominant markers (SCoT, ISSR and RAPD) for diagnostic fingerprinting in tetraploid potato. American J. Potato Res. 2011; 88: 226-237. [DOI:10.1007/s12230-011-9187-2]
13. Grime J, Benefits of plant diversity to ecosystems: immediate, filter and founder effects. J. Ecol. 2002; 86: 902-910. [DOI:10.1046/j.1365-2745.1998.00306.x]
14. Hagel JM, MacLeod BP and Facchini PJ. Opium poppy. Biotechnol. Agric. Forest. 2007; 61: 169-187.
15. Kalia RK, Rai MK, Kalia S, Singh R and Dhawan AK. Microsatellite markers: an Overview of the recent progress in plants. Euphytica. 2011; 177: 309-334. [DOI:10.1007/s10681-010-0286-9]
16. Krenn L, Glantschnig S and Sorger U. Determination of the five major opium alkaloids by reversed phase high-performance liquid chromatography on a base-deactivated stationary phase. Chromatographia 1998; 47: 21-24. [DOI:10.1007/BF02466781]
17. Kyslikova E, Babiak P, Stepanek V, Zahradnik J, Palyzova A, Maresova H, Valesova R, Hajicek J and Kyslik P. Biotransformation of codeine to 14-OHcodeine derivatives by Rhizobium radiobacter R.89-1. J. Molecular Catalysis B: Enzymatic. 2013; 87: 1-5. [DOI:10.1016/j.molcatb.2012.10.004]
18. Milo J, Levy A and Palevitch D. The cultivation and breeding of P. bracteatum. In: J. Bernath (Editor) Poppy - The Genus Papaver, Harwood Academic Publisher, Amesterdam. 1998; pp: 279 - 289.
19. Mirzaei K and Mirzaghaderi G. Genetic diversity analysis of Iranian Nigella sativa L. landraces using SCoT markers and evaluation of adjusted polymorphism information content. Plant Genetic Resources 2017; 15: 64-71. [DOI:10.1017/S1479262115000386]
20. Mohammadi S and Prasanna B. Analysis of genetic diversity in crop plants-salient statistical tools and considerations. Crop Science 2003; 43: 1235-1248. [DOI:10.2135/cropsci2003.1235]
21. Nakagawa A, Minami H, Kim JS, Koyanagi T, Katayama T, Sat F and Kumagai H. A bacterial platform for fermentative production of plant alkaloids. Nature Communications 2011; 2: 1-9. [DOI:10.1038/ncomms1327]
22. Newman DJ and Cragg GM. Natural products as sources of new drugs over the 30 years from 1981 to 2010. J. Nat. Prod. 2012; 75: 311-335. [DOI:10.1021/np200906s]
23. Niknam S, Faramarzi MA, Abdi K, Yazdi MT, Amini M and Rastegar H. Bioconversion of codeine to semi-synthetic opiate derivatives by the cyanobacterium Nostoc muscorum. World J. Microbiol. Biotechnol. 2010; 26: 119-123. [DOI:10.1007/s11274-009-0150-z]
24. Nyman U. Selection for high thebaine/low morphine content (cpv. Morph: The) in Papaver somniferum L. Hereditas 1978; 89: 43-50. [DOI:10.1111/j.1601-5223.1978.tb00979.x]
25. Pritchard JK, Stephens M and Donnelly P. Inference of Population Structure Using Multilocus Genotype Data. Genet. 2000; 155: 945-959.
26. Rajesh MK, Sabana AA, Rachana KE, Rahman S, Jerard BA and Karun A. Genetic relationship and diversity among coconut (Cocos nucifera L.) accessions revealed through SCoT analysis. 3Biotech. 2015; 5: 999-1006. [DOI:10.1007/s13205-015-0304-7]
27. Sharghi N and Lalezari I. Papaver bracteatum Lindl. a Highly Rich Source of Thebaine. Nature. 1967; 213: 1244. [DOI:10.1038/2131244a0]
28. Gurkok T, Kaymak E, Boztepe G, Koyuncu M and Parmaksiz I. Molecular characterization of the genus Papaver section Oxytona using ISSR markers. Turk. J. Bot. 2013; 37: 644-650. [DOI:10.3906/bot-1208-16]
29. Mohseni Z, Bernousi I, Mandoulakani B A and Darvishzadeh R. Genetic Diversity in Papaver bracteatum and Papaver somniferum Populations Revealed by ISSR Markers. Bulgarian J. Agricultural Science 2015; 21: 485-493.

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Medicinal Plants

Designed & Developed by : Yektaweb