year 19, Issue 73 (3-2020)                   J. Med. Plants 2020, 19(73): 163-169 | Back to browse issues page

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Hajihossein R, Eslamirad Z, Rafiei F, Naderi G, Assadi M. Anti-Acanthamoeba effect of Camellia sinensis extract (black and green tea) in vitro. J. Med. Plants 2020; 19 (73) :163-169
1- Department of Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, Iran
2- Department of Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, Iran ,
3- Department of Biostatistics, School of Medicine, Arak University of Medical Sciences, Arak, Iran
4- Department of Agriculture and Natural Resources, Islamic Azad University, Arak Branch, Arak, Iran
5- Department of Biochemistry, Islamic Azad University, Tehran Branch, Tehran, Iran
Abstract:   (1731 Views)
Background: Acanthamoeba is a resistant protozoan that causes severe diseases, such as GAE and CAK. Because many medications are ineffective on the parasite, the quest to find alternative drugs is in progress. Objective: This research was aim ed to assess the performance of the black and green tea extracts on Acanthamoeba. Methods: The clinical isolate of Acanthamoeba was cultured on non-nutrient agar plates. The black and green tea extracts were prepared via maceration, dried by rotary evaporation, and stored at 4 °C. The main component of the extracts (caffeine) was recognized using GC-MS analysis. The effects of three concentrations of black and green tea extracts were evaluated on the parasite and compared with the control and current drug. Results: Black and green tea extracts have lethal effects on Acanthamoeba cysts and the performance was more than that shown by the control and the current anti-keratitis drug. Moreover, the effectiveness of the tea extracts was tim e- and dose-dependent (P < 0.0001). There was no significant differences between the performance of black and green tea (P > 0.05). Conclusion: Black  and green tea extracts demonstrated the potential to inhibit the Acanthamoeba parasite, but the use of tea extract in clinical applications requires further study.
Full-Text [PDF 347 kb]   (818 Downloads)    
Type of Study: Research | Subject: Pharmacognosy & Pharmaceutics
Received: 2018/11/11 | Accepted: 2019/08/4 | Published: 2020/06/6

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