year 19, Issue 73 (3-2020)                   J. Med. Plants 2020, 19(73): 163-169 | Back to browse issues page


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Hajihossein R, Eslamirad Z, Rafiei F, Naderi G, Assadi M. Anti-Acanthamoeba effect of Camellia sinensis extract (black and green tea) in vitro. J. Med. Plants. 2020; 19 (73) :163-169
URL: http://jmp.ir/article-1-2346-en.html
1- Department of Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, Iran
2- Department of Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, Iran , z.eslami64@gmail.com
3- Department of Biostatistics, School of Medicine, Arak University of Medical Sciences, Arak, Iran
4- Department of Agriculture and Natural Resources, Islamic Azad University, Arak Branch, Arak, Iran
5- Department of Biochemistry, Islamic Azad University, Tehran Branch, Tehran, Iran
Abstract:   (762 Views)
Background: Acanthamoeba is a resistant protozoan that causes severe diseases, such as GAE and CAK. Because many medications are ineffective on the parasite, the quest to find alternative drugs is in progress. Objective: This research was aim ed to assess the performance of the black and green tea extracts on Acanthamoeba. Methods: The clinical isolate of Acanthamoeba was cultured on non-nutrient agar plates. The black and green tea extracts were prepared via maceration, dried by rotary evaporation, and stored at 4 °C. The main component of the extracts (caffeine) was recognized using GC-MS analysis. The effects of three concentrations of black and green tea extracts were evaluated on the parasite and compared with the control and current drug. Results: Black and green tea extracts have lethal effects on Acanthamoeba cysts and the performance was more than that shown by the control and the current anti-keratitis drug. Moreover, the effectiveness of the tea extracts was tim e- and dose-dependent (P < 0.0001). There was no significant differences between the performance of black and green tea (P > 0.05). Conclusion: Black  and green tea extracts demonstrated the potential to inhibit the Acanthamoeba parasite, but the use of tea extract in clinical applications requires further study.
Full-Text [PDF 347 kb]   (323 Downloads)    
Type of Study: Research | Subject: Pharmacognosy & Pharmaceutics
Received: 2018/11/11 | Accepted: 2019/08/4 | Published: 2020/06/6

References
1. Marciano-Cabral F and Cabral G. Acanthamoeba spp. as Agents of Disease in Humans. Clin. Microbiol. Rev. 2003; 16 (2): 273-307. DOI: 10.1128/cmr.16.2.273-307.2003. [DOI:10.1128/CMR.16.2.273-307.2003]
2. Paltiel M, Powell E, Lynch J, Baranowski B and Martins C. Disseminated cutaneous acanthamebiasis: a case report and review of the literature. Cutis. 2004; 73 (4): 241-8. [PMID:15134324].
3. Rezeaian M, Farnia S, Niyyati M and Rahimi F. Amoebic keratitis in Iran (1997-2007). Iran. J. Parasitol. 2007; 2 (3): 1-6.
4. Niyyati M and Rezaeian M. Current Status of Acanthamoeba in Iran: A Narrative Review Article. Iran. J. Parasitol. 2015; 10 (2): 157-63. [PubMed: 26246812].
5. Lorenzo-Morales J, Khan NA and Walochnik J. An update on Acanthamoeba keratitis: diagnosis, pathogenesis and treatment. Parasite. 2015; 22: 10. DOI: 10.1051/parasite/2015010. [PMC4330640]. [DOI:10.1051/parasite/2015010]
6. Malatyali E, Tepe B, Degerli Sand Berk S. In vitro amoebicidal activities of Satureja cuneifolia and Melissa officinalis on Acanthamoeba castellanii cysts and trophozoites. Parasitol Res. 2012; 110 (6): 2175-80. DOI: 10.1007/s00436-011-2744-2. [PubMed: 22160280]. [DOI:10.1007/s00436-011-2744-2]
7. Anita P, Sivasamy S, Madan Kumar PD, Balan IN and Ethiraj S. In vitro antibacterial activity of Camellia sinensis extract against cariogenic microorganisms. J. Basic Clin. Pharm. 2014; 6 (1): 35-9. DOI: 10.4103/0976-0105.145777. [PubMed: 25538470]. [DOI:10.4103/0976-0105.145777]
8. Sharangi A. Medicinal and therapeutic potentialities of tea (Camellia sinensis L.)-A review. Food Research International. 2009; 42 (5): 529-35. DOI: 10.1016/j.foodres.2009.01.007. [DOI:10.1016/j.foodres.2009.01.007]
9. Niyyati M, Dodangeh S and Lorenzo-Morales J. A Review of the Current Research Trends in the Application of Medicinal Plants as a Source for Novel Therapeutic Agents Against Acanthamoeba Infections. Iran. J. Pharm. Res. 2016; 15 (4): 893-900. DOI: [PMID:28243287].
10. Degerli S, Tepe B, Celiksoz A, Berk S and Malatyali E. In vitro amoebicidal activity of Origanum syriacum and Origanum laevigatum on Acanthamoeba castellanii cysts and trophozoites. Exp. Parasitol. 2012 5; 131 (1): 20-4. DOI: 10.1016/j.exppara.2012.02.020. [PubMed: 22417972]. [DOI:10.1016/j.exppara.2012.02.020]
11. Azmir J, Zaidul ISM, Rahman MM, Sharif KM, Mohamed A, Sahena F and et al. Techniques for extraction of bioactive compounds from plant materials: A review. J. Food Engineering. 2013; 117 (4): 426-36. DOI: 10.1016/j.jfoodeng.2013.01.014. [DOI:10.1016/j.jfoodeng.2013.01.014]
12. Siddiqui R and Khan NA. Photochemotherapeutic strategies against Acanthamoeba keratitis. AMB Express. 2012; 2: 47. DOI: 10.1186/2191-0855-2-47. [PubMed:3496604]. [DOI:10.1186/2191-0855-2-47]
13. Ibrahim YW, Boase DL and Cree IA. How Could Contact Lens Wearers Be at Risk of Acanthamoeba Infection? A Review. J. Optom. 2009; 2 (2): 60-6. DOI: 10.3921/joptom.2009.60. [PubMed:3972779]. [DOI:10.3921/joptom.2009.60]
14. Ibrahim YW, Boase DL and Cree IA. How Could Contact Lens Wearers Be at Risk of Acanthamoeba Infection? A Review. J. Optom. 2009; 2 (2): 60-6. DOI: 10.3921/joptom.2009.60. [PubMed:3972779] [DOI:10.3921/joptom.2009.60]
15. Todd CD, Reyes-Batlle M, Martin-Navarro CM, Dorta-Gorrin A, Lopez-Arencibia A, Martinez-Carretero E and et al. Isolation and genotyping of acanthamoeba strains from soil sources from Jamaica, West Indies. J. Eukaryot. Microbiol. 2015; 62 (3): 416-21. DOI: 10.1111/jeu.12197. [PubMed: 25393552]. [DOI:10.1111/jeu.12197]
16. Kim KY, Davidson PM and Chung HJ. Antibacterial activity in extracts of Camellia japonica L. petals and its application to a model food system. J. Food Prot. 2001; 64 (8): 1255-60. [PubMed: 11510672]. [DOI:10.4315/0362-028X-64.8.1255]
17. Yildirim A, Mavi A, Oktay M, Kara AA, Algur ÖF and Bilaloglu V. Comparison of antioxidant and antimicrobial activities of Tilia (Tilia argentea Desf ex DC), sage (Salvia triloba L.), and Black tea (Camellia sinensis) extracts. J. Agric. Food Chem. 2000; 48 (10): 5030-4. DOI: 10.1021/jf000590k. [DOI:10.1021/jf000590k]
18. Chan EWC, Soh EY, Tie PP and Law YP. Antioxidant and antibacterial properties of green, black, and herbal teas of Camellia sinensis. Pharmacognosy Res. 2011; 3 (4): 266-72. DOI: 10.4103/0974-8490.89748. [PubMed: PMC3249787]. [DOI:10.4103/0974-8490.89748]
19. Memarzadeh S, Validi M, Mobini G, Rafeian-Kopaei M and Mansouri S. The antibacterial effect of camellia sinensis extract on bacterias, conjunctivitis in vitro. J. Shahrekord Uuniversity of Medical Sciences 2012; 14 (4): 61-9. [In Persian]
20. Sardari F and Hajisadeghi S. Comparison of the Antimicrobial Efficacy of Green Tea Extract With 1% Sodium Hypochlorite Against Enterococcus faecalis: An In Vitro Study. Jundishapur J. Nat. Pharm. Prod. 2016; 11 (4): e30944. DOI: 10.17795/jjnpp-30944. [PubMed: 20003940]. [DOI:10.17795/jjnpp-30944]

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