year 19, Issue 74 (6-2020)
J. Med. Plants 2020, 19(74): 108-117 |
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Karami A, Hamzeloo-Moghadam M, Yami A, Barzegar M, Mashati P, Gharehbaghian A. The Effect of Gaillardin on Proliferation and Apoptosis of Acute Lymphoblastic Leukemia Cell Line (Nalm-6). J. Med. Plants 2020; 19 (74) :108-117
URL: http://jmp.ir/article-1-2251-en.html
URL: http://jmp.ir/article-1-2251-en.html
Afshin Karami1 
, Maryam Hamzeloo-Moghadam2 , Amir Yami1 , Mohyedin Barzegar1 , Pargol Mashati1 , Ahmad Gharehbaghian * 3
, Maryam Hamzeloo-Moghadam2 , Amir Yami1 , Mohyedin Barzegar1 , Pargol Mashati1 , Ahmad Gharehbaghian * 3
1- Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Pediatric Congenital Hematologic Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran , gharehbaghian@sbmu.ac.ir
2- Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Pediatric Congenital Hematologic Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran , gharehbaghian@sbmu.ac.ir
Abstract: (2547 Views)
Background: Due to increased cancer chemotherapy resistance, the use of natural compounds in cancer treatment has been considered in recent years. Inula-Oculus-Christi, is one of the high importance plants in the field of traditional medicine. It has been shown that extract of this plant has cytotoxic effects on breast cancer cells.
Objective: Therefore, the aim of this study was to evaluate the cytotoxic effect of Gaillardin and apoptosis induced by this extract on acute lymphoblastic leukemia cells. Methods: Gaillardin from Inula-Oculus-Christi plant was extracted by Traditional Medicine Research Center of Shahid Beheshti University of Medical Sciences. The cytotoxic effect of Gaillardin on the acute lymphoblastic leukemia (Nalm-6) and PBMC (as a normal cell line) was investigated at different doses for 24 and 48 hours. In this study, Trypan blue, MTT assay and DAPI staining were used. In addition, Annexin V/PI staining was utilized for confirming apoptosis.
Results: The results of Trypan blue and MTT assay demonstrated the induction of apoptosis and reduction in proliferation of Nalm-6 cells treated by Gaillardin, showing a time and dose-dependent manner (P < 0.001). However, low sensitivity to the different concentrations of Gaillardin was observed in PBMCs. DAPI staining and Flow cytometry analysis confirmed a significantly high percentage of apoptotic cells compared with control groups (P < 0.001). Conclusion: According to the results, Gaillardin demonstrated cytotoxic effects on Nalm-6 cells, but
Results: The results of Trypan blue and MTT assay demonstrated the induction of apoptosis and reduction in proliferation of Nalm-6 cells treated by Gaillardin, showing a time and dose-dependent manner (P < 0.001). However, low sensitivity to the different concentrations of Gaillardin was observed in PBMCs. DAPI staining and Flow cytometry analysis confirmed a significantly high percentage of apoptotic cells compared with control groups (P < 0.001). Conclusion: According to the results, Gaillardin demonstrated cytotoxic effects on Nalm-6 cells, but
Type of Study: Research |
Subject:
Traditional Pharmacy & Traditional Medicine
Received: 2018/09/2 | Accepted: 2019/01/28 | Published: 2020/07/21
Received: 2018/09/2 | Accepted: 2019/01/28 | Published: 2020/07/21
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