year 12, Issue 47 (9-2013)                   J. Med. Plants 2013, 12(47): 100-110 | Back to browse issues page

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Qaderi A, Omidi M, Zebarjadi A, Hajiaghaee R. Over - expression Effect of Gene Encoding 3-hydroxy-3-Methylglutaryl-CoA Reductase on Production of Taxol in Iranian Hazel (Corylus avellana L.). J. Med. Plants 2013; 12 (47) :100-110
1- Department of Plant Breeding, Science and Research Branch, Islamic Azad University ,
2- Department of Agronomy and Plant Breeding, Faculty of Agriculture, Tehran University
3- Agronomy and Plant Breeding Department, Faculty of Agriculture, Razi University
4- Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
Abstract:   (5556 Views)
Background: Sustainable and commercial production of taxol as an anti cancer drug is a critical point to its clinical application. Nowadays, hazel because of rapid growth and wide range distribution is considered as an alternative source of Taxol. Objective: To increase taxol production the cDNA encoding 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) from Iranian hazel (GeneBank accession number KF306244, showed by CiHMGR) was isolated and over-expressed in pCAMBIA1304 binary vector. The effect of transient over-expression of HMGR in callus and leaf were evaluated on Taxol production. Methods: The calli was established through the culture of immature cotyledon on Murashige and Skoog basal medium supplemented with 2, 4-D and BA. The first strand cDNA of CiHMGR was synthesized by specific primers. Enzymatic assay of recombinant CiHMGR in E. coli were done by western blott and His-tag affinity techniques. Also production of taxol in transformed callus and leaf were evaluated by HPLC analysis. Results: An Open Reading Frame (ORF) with 1698 bp length and a deduced polypeptide with 566 amino acid residues were amplified. The highest and lowest amount of taxol was 0.016 mg/g.DW and 0.004 mg/gDW in transformed calli and untransformed leaves respectively. Conclusion: Generally the over-expression of HMGR increase the total isoprenoids yield, therefore to have high production of target secondary metabolites (taxol) we need both of network of transformed genes and elicited cell culture.
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Type of Study: Research | Subject: Biotechnology
Received: 2013/06/22 | Accepted: 2013/07/10 | Published: 2013/10/22

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