year 3, Issue 9 (2-2004)                   J. Med. Plants 2004, 3(9): 55-68 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Haidary R, Shaheen F, Sombati S, Lorenzo R. Inhibition of sustained repetitive firing (SRF) in cultures hippocampal neurons by an aqueous fraction isolated from Delphinium denudatum WALL.ex Hook.F.&Thoms. J. Med. Plants. 2004; 3 (9) :55-68
URL: http://jmp.ir/article-1-755-en.html
1- Department of Neurology, Medical College of Virginia, Virginia Commonwealth University, Virginia, USA , mrheydari@sina.tums.ac.ir
2- H.E.J. Research Institute of Chemistry, University of Karachi, Karachi, Pakestan
3- 1- Department of Neurology, Medical College of Virginia, Virginia Commonwealth University, Virginia, USA
4- Department of Neurology, Medical College of Virginia, Virginia Commonwealth University, Virginia, USA
Abstract:   (6007 Views)
In this report we investigated the effects of the aqueous fraction (AF) isolated from Delphinium denudatum on Sustained Repetitive Firing (SRF) in cultured neonatal rat hippocampal pyramidal neurons. Blockade of SRF is one of the basic mechanisms of antiepileptic drugs (AED) at the cellular level. The effects of aqueous fraction (0.2-0.6 mg/ml) were compared with the prototype antiepileptic drug, phenytoin (PHT). Using the whole cell current-clamp technique, Sustained Repetitive Firing was elicited in neurons by a depolarizing pulse of 500 ms duration, 0.3 Hz and 0.1-0.6 nA current strength. Similar to phenytoin, aqueous fraction reduced the number of action potentials (AP) per pulse in a concentration-dependent manner until no action potentials were elicited for the remainder of the pulse. There was a corresponding use-dependent reduction in amplitude and Vmax (velocity of upstroke) of action potentials. The Vmax and amplitude of the first action potential was not affected by phenytoin, while aqueous fraction exhibited concentration-dependent reduction. At 0.6 mg/ml aqueous fraction reduced Vmax to 58-63 % and amplitude to 16-20 % of the control values. The blockade of Sustained Repetitive Firing by aqueous fraction was reversed with hyperpolarization of membrane potential (-65 to –75 mV) while depolarization of membrane potential (-53mV to -48 mV) potentiated the block. The results suggest that aqueous fraction blocks Sustained Repetitive Firing in hippocampal neurons in a use-dependent and voltage-dependent manner similar to phenytoin. However, unlike phenytoin, which interacts preferably with the inactive state of the Na+ channel, the compounds present in aqueous fraction apparently also interact with the resting state of the Na+ channels as suggested by dose-dependent reduction of Vmax and amplitude of first AP. We conclude that aqueous fraction contains potent anticonvulsant compounds.
Full-Text [PDF 354 kb]   (1898 Downloads)    
Type of Study: Research | Subject: Pharmacology & Toxicology
Received: 2002/11/3 | Accepted: 2004/01/30 | Published: 2004/03/9

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2021 CC BY-NC 4.0 | Journal of Medicinal Plants

Designed & Developed by : Yektaweb