year 22, Issue 86 (5-2023)
J. Med. Plants 2023, 22(86): 44-61 |
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Rafiee H, Mehrafarin A, Naghdi Badi H, Khalighi-Sigaroodi F, Jabbari E, Ramawat N. Alleviation of low temperatures injury on lemon verbena (Lippia citriodora H.B.K.) by exogenous application of adjuvants in anti-chilling formulations. J. Med. Plants 2023; 22 (86) :44-61
URL: http://jmp.ir/article-1-3510-en.html
URL: http://jmp.ir/article-1-3510-en.html
Hanieh Rafiee1 
, Ali Mehrafarin * 2, Hassanali Naghdi Badi3 , Farahnaz Khalighi-Sigaroodi4 , Esmaiel Jabbari5 , Naleeni Ramawat6
, Ali Mehrafarin * 2, Hassanali Naghdi Badi3 , Farahnaz Khalighi-Sigaroodi4 , Esmaiel Jabbari5 , Naleeni Ramawat6
1- Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran
2- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran; Medicinal Plants Research Center, Shahed University, Tehran, Iran , Mehrafarin@imp.ac.ir
3- Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahed University, Tehran-Qom Express Way, Tehran, Iran; Medicinal Plants Research Center, Shahed University, Tehran, Iran
4- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
5- Biomimetic Materials and Tissue Engineering Laboratories, Department of Chemical Engineering, University of South Carolina, Columbia, South Carolina 29208, United States
6- Department of Agronomy, Agriculture University Jodhpur, Rajasthan, India
2- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran; Medicinal Plants Research Center, Shahed University, Tehran, Iran , Mehrafarin@imp.ac.ir
3- Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahed University, Tehran-Qom Express Way, Tehran, Iran; Medicinal Plants Research Center, Shahed University, Tehran, Iran
4- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
5- Biomimetic Materials and Tissue Engineering Laboratories, Department of Chemical Engineering, University of South Carolina, Columbia, South Carolina 29208, United States
6- Department of Agronomy, Agriculture University Jodhpur, Rajasthan, India
Abstract: (600 Views)
Background: Lemon verbena (Lippia citriodora H.B.K) from Verbenaceae is a sensitive plant to low temperatures as abiotic stress. Objective: The objective of this research was to evaluate the effects of adjuvants and anti-chilling formulations on lemon verbena (Lippia citriodora H.B.K) leaves under low temperatures. Methods: The combined analysis was done on the basis of a randomized complete blocks design (RCBD) with 28 treatments and 3 replications. The three factors included two anti-chilling formulations (glycerol, and glycerol + polyvinyl alcohol), seven adjuvants formulations (α-tocopherol, amino acids of proline + glycine-betaine, and ABA), and two levels of low temperature (5 and 10 °C). Results: The treatment of glycerol + proline + glycine-betaine + ABA reduced the damaging effects of low temperature in biomass, essential oil content, and osmoprotectants, while the highest protection by antioxidant pigments was obtained in glycerol + α-tocopherol + ABA. Enzymes activities and polyphenol content showed the best results by glycerol + ABA. Conclusion: The best formulations were glycerol + proline + glycine-betaine + ABA and glycerol + ABA from the viewpoint of economic yield and also qualitative protection against low temperature, respectively. The efficiency of the mentioned formulations was due to their direct protective function and the indirect influence of adjuvants in a synergistic interaction with other components of the formulation.
Keywords: Lippia citriodora H.B.K., Glycerol, Proline, Glycine-betaine, α-Tocopherol, Abscisic acid, Citric acid
Type of Study: Research |
Subject:
Medicinal Plants
Received: 2023/04/23 | Accepted: 2023/07/16 | Published: 2023/05/31
Received: 2023/04/23 | Accepted: 2023/07/16 | Published: 2023/05/31
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