year 21, Issue 84 (12-2022)                   J. Med. Plants 2022, 21(84): 50-64 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Naderian P, Moshtaghi N, Bagheri A, Malekzadeh-Shafaroudi S. Influence of different Agrobacterium rhizogenes strains on hairy roots induction and secondary metabolites production in Datura innoxia Mill.. J. Med. Plants 2022; 21 (84) :50-64
1- MSc of Agricultural Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
2- Department of Biotechnology and Plant Breeding, Ferdowsi University of Mashhad, Mashhad, Iran ,
3- Department of Biotechnology and Plant Breeding, Ferdowsi University of Mashhad, Mashhad, Iran
Abstract:   (1038 Views)
Background: Datura innoxia Mill. is one of the medicinal plant which can produce tropan alkaloids such as hyoscyamine and scopolamine with analgesic and antiasthmatic activities. Objective: In this research, the effect of various factors such as culture media and bacteria strain were studied on hairy roots induction and secondary metabolites production in Datura innoxia. Methods: Strains A4, A13, R1000, MSU, 15834, 2656 and 11325 of Agrobacterium rhizogenes with two different concentrations (OD = 0.5, 1) used for co-cultivation of leaf and stem explants prepared from 1-3-month-old and 5-7-month-old plants to optimize the production of hairy roots and then 6 different media were used for establishment and growth of hairy roots. Results: The results of data analysis showed that A4 strain with 86.6 % hairy root production was the best strain than others. Also, dark conditions, younger explants and higher concentration of bacteria caused the highest amount of induction of hairy roots. ½ MS medium was recognized as the best medium for the growth of hairy roots. The results of HPLC analysis also showed that the level of hyoscyamine in hairy roots was lower than that of normal roots which, of course, can be compensated by the higher growth of hairy roots.  Conclusion: The appearance and growth of hairy roots depends on various factors such as bacterial strain, culture medium, explant and its age and other factors that need to be optimized at the beginning of any research.
Full-Text [PDF 701 kb]   (622 Downloads)    
Type of Study: Research | Subject: Medicinal Plants
Received: 2022/08/28 | Accepted: 2022/10/26 | Published: 2022/12/1

1. Mardare (Balusescu) G, Malutan T and Lazar L. Determination of scopolamine by gas chromatography from different parts of the Datura innoxia biomass. Rev. Chim. 2020; 71(7): 126-136. [DOI:10.37358/RC.20.7.8230]
2. Lounasmaa M and Tamminen T. The Tropane Alkaloids. In Brossi A (ed.), The Alkaloids, New York: Academic; 1993: 1-113. [DOI:10.1016/S0099-9598(08)60143-1]
3. Li C and Wang M. Application of Hairy Root Culture for Bioactive Compounds Production in Medicinal Plants. Curr. Pharm. Biotechnol. 2021; 22(5): 592-608. [DOI:10.2174/1389201021666200516155146]
4. Sevón N and Oksman-Caldentey KM. Agrobacterium rhizogenes-mediated transformation: root cultures as a source of alkaloids. Planta Med. 2002; 68(10): 859-68. [DOI:10.1055/s-2002-34924]
5. Chang SS, Park SK, Kim BC, Kang BJ, Kim DU and Nam HG. Stable genetic transformation of Arabidobsis thaliana by Agrobacterium inoculation in planta. The Plant J. 1994; 5(4): 551-558. [DOI:10.1046/j.1365-313X.1994.5040551.x]
6. Guillonl S, Jocelyne TG, Pati Kumar PK, Rideau M and Gantet. Hairy root research: recent scenario and exciting prospects. Curr. Opin. Plant Biol. 2006; 9(3): 341-346. [DOI:10.1016/j.pbi.2006.03.008]
7. Torregrosa L and Bouquet A. Agrobacterium rhizogenes and A. tumefacience co-transformation to obtain grapevine hairy roots producing the coat protein of grapevine chrome mosaic nepovirus. PCTOC. 1997; 49: 53-63. [DOI:10.1023/A:1005854212592]
8. Rao SR and Ravishankar GA. Plant cell cultures: chemical factories of secondary metabolites. Biotechnol. Adv. 2002; 20(2): 101-153. [DOI:10.1016/S0734-9750(02)00007-1]
9. Thiruvengadam M, Praveen N, John KMM, Yang YS, Kim SH and Chung IM., Establishment of Momordica charantia hairy root cultures for the production of phenolic compounds and determination of their biological activities. PCTOC. 2014; 118(3): 545-557. [DOI:10.1007/s11240-014-0506-4]
10. Huang P, Xia L, Liu W, Jiang R, Liu X, Tang Q, Xu M, Yu L, Tang Z and Zeng J. Hairy root induction and benzylisoquinoline alkaloid production in Macleaya cordata. Sci. Rep. 2018; 8: 11986. [DOI:10.1038/s41598-018-30560-0]
11. Häkkinen ST, Raven N, Henquet M, Laukkanen ML, Anderlei T, Pitkänen JP, Twyman RM, Bosch D, Oksman-Caldentey KM, Schillberg S and Ritala A. Molecular farming in tobacco hairy roots by triggering the secretion of a pharmaceutical antibody. Biotechnol. Bioeng. 2013; 111(2): 336-346. [DOI:10.1002/bit.25113]
12. Ele Ekouna JP, Boitel-Conti M, Lerouge P, Bardor M and Guerineau F. Enhanced production of recombinant human gastric lipase in turnip hairy roots. PCTOC. 2017; 131: 601-610. [DOI:10.1007/s11240-017-1309-1]
13. Gutierrez-Valdes N, Häkkinen ST, Lemasson C, Guillet M, Oksman-Caldentey KM, Ritala A and Cardon F. Hairy root cultures-a versatile tool with multiple applications. Front. Plant Sci. 2020; 11: 33. [DOI:10.3389/fpls.2020.00033]
14. Murashige T and Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 1962; 15(3): 473-497. [DOI:10.1111/j.1399-3054.1962.tb08052.x]
15. Gamborg OL, Miller RA, Ojima K. Nutrient requirements of suspension cultures of soyabean root cells. Exp. Cell Res. 1968; 50(1): 151-158. [DOI:10.1016/0014-4827(68)90403-5]
16. Spurina V, Sovova M, Jirmanova E and Sustackova A. Chromosomal characteristics and occurrence of main alkaloids in Datura stramonium and Datura wrightii. Planta Med. 1981; 41(4): 366-373. [DOI:10.1055/s-2007-971728]
17. Bonhomme V, Laurain-Mattar D, Lacoux J, Fliniaux MA and Jacquin-Dubreuil A. Tropane alkaloid production by hairy roots of Atropa belladonna obtained after transformation with Agrobacterium rhizogenes 15834 and Agrobacterium tumefaciens containing rolA, B, C genes only. J. Biotech. 2000; 81(2-3): 151-158. [DOI:10.1016/S0168-1656(00)00287-X]
18. Veena V and Taylor CG. Agrobacterium rhizogenes: recent developments and promising applications. In Vitro Cell Dev. Bio. Plant 2007; 43: 383-403. [DOI:10.1007/s11627-007-9096-8]
19. Farsi M and Zolala J. Plant Biotechnology. ACECR - Mashhad Branch Publication. 2003; pp: 87-120. [In Persian]
20. Farsi M, Moshtaghi N, Shahriari Ahmadi F, Gordan H and Raisi M. Investigating growth stability and alkaloid content of transgenic hairy roots in Datura plant. Agric. Sci. Ind. 2005; 19(2): 47-56. [In Persian]
21. Dehghan E, Häkkinen ST, Oksman-Caldentey KM and Shahriari Ahmadi F. Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian henbane (Hyoscyamus muticus L.). PCTOC. 2012; 110: 35-44. [DOI:10.1007/s11240-012-0127-8]
22. Georgiev MI, Pavlov AI and Bley T. Hairy root type plant in vitro systems as sources of bioactive substances. Appl. Microbiol. Biotechnol. 2007; 74: 1175-1185. [DOI:10.1007/s00253-007-0856-5]
23. Sathasivam R, Choi M, Radhakrishnan R, Kwon H, Yoon J, Yang SH, Kim JK, Chung YS and Park SU. Effects of various Agrobacterium rhizogenes strains on hairy root induction and analyses of primary and secondary metabolites in Ocimum basilicum. Front. Plant Sci. 2022; 13: 983776. [DOI:10.3389/fpls.2022.983776]
24. Thwe A, Arasu MV, Li X, Park CH, Kim SJ, Al-Dhabi NA and Park SU. Effect of different Agrobacterium rhizogenes strains on hairy root induction and phenylpropanoid biosynthesis in tartary buckwheat (Fagopyrum tataricum gaertn). Front. Microbiol. 2016; 7: 318. [DOI:10.3389/fmicb.2016.00318]
25. Hamidi Shirghan E, Moshtaghi N, Malekzadeh-Shafaroudi S, Bagheri A and Sharifi A. Effect of the type and concentration of Agrobacterium rhizogenes bacteria on the percentage of hairy root emergence in Periwinkle plant. 8th Biotechnology Conference and 4th National Biosafety Conference. 2013.
26. Fan Y, Xu F, Zhou H, Liu X, Yang X, Weng K, Sun X and Lyu S. A fast, simple, high efficient and one-step generation of composite cucumber plants with transgenic roots by Agrobacterium rhizogenes-mediated transformation. PCTOC. 2020; 141: 207-216. [DOI:10.1007/s11240-020-01781-x]
27. Palazon J, Navarro-Ocana A, Hernandez-Vazquez L and Mirjalali MH. Application of metabolic engineering to the production of Scopolamine. Molecules 2008; 13(8): 1722-1742. [DOI:10.3390/molecules13081722]
28. Silva-Navas J, Moreno-Risueno MA, Manzano C, Pallero-Baena M, Navarro- Neila S, Téllez-Robledo B, Garcia-Mina JM, Baigorri R, Gallego FJ and del Pozo JC. D-Root: a system for cultivating plants with the roots in darkness or under different light conditions. The Plant J. 2015; 84(1): 244-255. [DOI:10.1111/tpj.12998]
29. Costigan SE, Warnasooriya SN, Humphries BA and Montgomery BL. Root-localized phytochrome chromophore synthesis is required for photoregulation of root elongation and impacts root sensitivity to jasmonic acid in Arabidopsis. Plant Physiol. 2011; 157(3): 1138-1150. [DOI:10.1104/pp.111.184689]
30. Correll MJ and Kiss JZ. The roles of phytochromes in elongation and gravitropism of roots. Plant Cell Physiol. 2005; 46(2): 317-323. [DOI:10.1093/pcp/pci038]
31. Liu CZ, Wang YC, Zhao B, Guo C, Ouyang F, Ye HC and Li GF. Development of a nutrient mist bioreactor for growth of hairy roots. In Vitro Cell. Dev. Biol. 1999; 35(3): 271-274. []. [DOI:10.1007/s11627-999-0091-0]
32. Moyano E, Fornale S, Palazon J, Cusido RM, Bagni N and Pinol MT. Alkaloid production in duboisia hybrid hairy root cultures over-expressing the pmt gene. Phytochem. 2002; 59(7): 697-700. [DOI:10.1016/S0031-9422(02)00044-4]
33. Sevon N, Biondi S, Bagni N and Oksman -Caldentey KM. Transgenic Hyoscyamus muticus. In: Biotechnology in Agriculture and Forestry 48 (Ed. By Y.P.S Bajaj). Berlin-Heidelberg: Springer velag; 2001: 171-200. [DOI:10.1007/978-3-662-10603-7_13]
34. Roy A. Hairy root culture an alternative for bioactive compound production from medicinal plants. Curr. Pharm. Biotechnol. 2021; 22(1), 136-149. [DOI:10.2174/18734316MTEyfNzcD0]
35. Traverse KKF, Mortensen S, Trautman JG, Danison H, Rizvi NF and Lee-Parsons CWT. Generation of stable Catharanthus roseus hairy root lines with Agrobacterium rhizogenes. Methods Mol. Biol. 2022; 2469: 129-144. [DOI:10.1007/978-1-0716-2185-1_11]
36. Naderian P, Moshtaghi N, Bagheri A, Malekzade Shafaroudi S. Variations in morphological, biochemical and phytochemical traits of diploid and induced tetraploid plants of downy thorn-apple (Datura innoxia Mill.). J. Med. Plants 2022; 21 (82) 66-79. [DOI:10.52547/jmp.21.82.66]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Journal of Medicinal Plants

Designed & Developed by : Yektaweb