year 21, Issue 83 (9-2022)
J. Med. Plants 2022, 21(83): 35-46 |
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Ghafourian S, Ghafourian E, Zarei S M, Jalilian F, Hosseinzadeh L, Ali Z, et al . Bioassay-guided isolation of phenolic apoptosis inducer anticancer agents from Berberis vulgaris fruits. J. Med. Plants 2022; 21 (83) :35-46
URL: http://jmp.ir/article-1-3336-en.html
URL: http://jmp.ir/article-1-3336-en.html
Sobhan Ghafourian1 
, Ehsan Ghafourian2 , Seyed Mohammad Zarei * 3, Fereshteh Jalilian4 , Leila Hosseinzadeh4 , Zulfiqar Ali5 , Yan-Hong Wang5 , Yalda Shokoohinia6
, Ehsan Ghafourian2 , Seyed Mohammad Zarei * 3, Fereshteh Jalilian4 , Leila Hosseinzadeh4 , Zulfiqar Ali5 , Yan-Hong Wang5 , Yalda Shokoohinia6
1- Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, IR Iran
2- Students Research Committee, Kermanshah University of Medical Sciences, Kermanshah, IR Iran
3- Department of Pharmacognosy and Traditional Pharmacy, Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, IR Iran ,sm.zarei@bmsu.ac.ir
4- Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, IR Iran
5- National Center for Natural Products Research, University of Mississippi, Oxford, MS, USA
6- Ric Scalzo Botanical Research Institute, Southwest College of Naturopathic Medicine, Tempe, AZ, USA
2- Students Research Committee, Kermanshah University of Medical Sciences, Kermanshah, IR Iran
3- Department of Pharmacognosy and Traditional Pharmacy, Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, IR Iran ,
4- Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, IR Iran
5- National Center for Natural Products Research, University of Mississippi, Oxford, MS, USA
6- Ric Scalzo Botanical Research Institute, Southwest College of Naturopathic Medicine, Tempe, AZ, USA
Abstract: (2295 Views)
Background: Cancer development is a multi-stage process in which apoptosis plays an important role. Apoptotic agents from natural products like phenolic compounds can be used effectively in the treatment of cancer. Berberis vulgaris L. fruits are a rich source of natural phenolic compounds and have been evaluated for their apoptotic effects. Objective: this study was carried out to evaluate the apoptotic effect of B. vulgaris fruits in normal and cancerous cells by investigating p53, Bax, and Bcl-2 genes expression and identifying an active compound. Methods: B. vulgaris fruits were extracted with ethanol using maceration method. To yield an active compound, the extract was divided into fractions and subfractions that could be evaluated using chromatography techniques, and the purified compound structure was determined by nuclear magnetic resonance (NMR). The cytotoxic activity of the selected fraction against 3T3 and MCF-7 cell lines along with its effect on the mRNA apoptosis regulating genes like p53, Bax, and Bcl-2 were investigated subsequently. Results: Results showed that B. vulgaris extract increased the mRNA level of p53 and Bax and decreased Bcl-2 mRNA level in cancer cell lines. The structure of the purified compound was determined as the 3-caffeoylquinic acid (chlorogenic acid). Conclusion: B. vulgaris fruit has in vitro antineoplastic activity by stimulating cellular apoptosis via increasing the levels of p53 and Bax proteins and decreasing Bcl-2 and comprises chlorogenic acid as one of its phenolic phytochemicals.
Type of Study: Research |
Subject:
Pharmacognosy & Pharmaceutics
Received: 2022/05/8 | Accepted: 2022/08/21 | Published: 2022/10/1
Received: 2022/05/8 | Accepted: 2022/08/21 | Published: 2022/10/1
References
1. Kumar M, Kaur V, Kumar S and Kaur S. Phytoconstituents as apoptosis inducing agents: strategy to combat cancer. Cytotechnol. 2016; 68(4): 531-563. [DOI:10.1007/s10616-015-9897-2]
2. Gali-Muhtasib H, Hmadi R, Kareh M, Tohme R and Darwiche N. Cell death mechanisms of plant-derived anticancer drugs: beyond apoptosis. Apoptosis. 2015; 20(12): 1531-1562. [DOI:10.1007/s10495-015-1169-2]
3. Camins A, Sureda FX, Junyent F, Verdaguer E, Folch J, Beas-Zarate C and Pallas M. An overview of investigational antiapoptotic drugs with potential application for the treatment of neurodegenerative disorders. Expert Opin. Investig. Drugs. 2010; 19(5): 587-604. [DOI:10.1517/13543781003781898]
4. Pfeffer CM and Singh ATK. Apoptosis: a target for anticancer therapy. Int. J. Mol. Sci. 2018; 19(2). [DOI:10.3390/ijms19020448]
5. Lin D, Xiao M, Zhao J, Li Z, Xing B, Li X, Kong M, Li L, Zhang Q, Liu Y, Chen H, Qin W, Wu H and Chen S. An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes. Molecules (Basel, Switzerland). 2016; 21(10). [DOI:10.3390/molecules21101374]
6. Aguilera Y, Martin-Cabrejas MA and González de Mejia E. Phenolic compounds in fruits and beverages consumed as part of the mediterranean diet: their role in prevention of chronic diseases. Phytochem. Rev. 2016; 15(3): 405-423. [DOI:10.1007/s11101-015-9443-z]
7. Mozaffarian V. A Dictionary of Iranian plant names: Latin-English-Persian. Seventh ed., Tehran: Farhang Moaser; 2013.
8. Mozaffarian V. Plant Systematics. Tehran: Nashr Danesh Emrouz; 1994, 372.
9. Rahimi-Madiseh M, Lorigoini Z, Zamani-Gharaghoshi H and Rafieian-Kopaei M. Berberis vulgaris: specifications and traditional uses. Iran. J. Basic Med. Sci. 2017; 20(5): 569-587.
10. Ghafourian E, Sadeghifard N, Pakzad I, Valizadeh N, Maleki A, Jafari F, Ghiasvand N, Abdi J, Shokoohinia Y and Ghafourian S. Ethanolic extract of Berberis vulgaris fruits inhibits the proliferation of MCF-7 breast cancer cell line through induction of apoptosis. Infect. Disord. Drug Targets. 2017; 17(3): 192-198. [DOI:10.2174/1871526517666170531113759]
11. Mansouri M, Moallem SA, Asili J and Etemad L. Cytotoxic and apoptotic effects of Scrophularia umbrosa Dumort extract on MCF-7 breast cancer and 3T3 cells. Rep. Biochem. Mol. Biol. 2019; 8(1): 79-84.
12. Islam MS, Yoshimoto M, Yahara S, Okuno S, Ishiguro K and Yamakawa O. Identification and characterization of foliar polyphenolic composition in sweetpotato (Ipomoea batatas L.) genotypes. J. Agric. Food Chem. 2002; 50(13): 3718-3722. [DOI:10.1021/jf020120l]
13. Simões-Pires CA, Queiroz EF, Henriques AT and Hostettmann K. Isolation and on-line identification of antioxidant compounds from three Baccharis species by HPLC-UV-MS/MS with post-column derivatisation. Phytochem. Anal. 2005; 16(5): 307-314. [DOI:10.1002/pca.826]
14. Salehi B, Selamoglu Z, Sener B, Kilic M, Kumar Jugran A, de Tommasi N, Sinisgalli C, Milella L, Rajkovic J, Flaviana BM-BM, C FB, J ER, H DMC, Oluwaseun Ademiluyi A, Khan Shinwari Z, Ahmad Jan S, Erol E, Ali Z, Adrian Ostrander E, Sharifi-Rad J, de la Luz Cádiz-Gurrea M, Taheri Y, Martorell M, Segura-Carretero A and Cho WC. Berberis plants-drifting from farm to food applications, phytotherapy, and phytopharmacology. Foods (Basel, Switzerland). 2019; 8(10). [DOI:10.3390/foods8100522]
15. El Khalki L, Tilaoui M, Jaafari A, Ait Mouse H and Zyad A. Studies on the dual cytotoxicity and antioxidant properties of Berberis vulgaris extracts and its main constituent berberine. Adv. Pharmacol. Sci. 2018. [DOI:10.1155/2018/3018498]
16. Gupta S, Afaq F and Mukhtar H. Involvement of nuclear factor-kappa B, Bax and Bcl-2 in induction of cell cycle arrest and apoptosis by apigenin in human prostate carcinoma cells. Oncogene. 2002; 21(23): 3727-3738. [DOI:10.1038/sj.onc.1205474]
17. Yang JS, Liu CW, Ma YS, Weng SW, Tang NY, Wu SH, Ji BC, Ma CY, Ko YC, Funayama S and Kuo CL. Chlorogenic acid induces apoptotic cell death in U937 leukemia cells through caspase- and mitochondria-dependent pathways. In Vivo (Athens, Greece). 2012; 26(6): 971-978.
18. Jin UH, Lee JY, Kang SK, Kim JK, Park WH, Kim JG, Moon SK and Kim CH. A phenolic compound, 5-caffeoylquinic acid (chlorogenic acid), is a new type and strong matrix metalloproteinase-9 inhibitor: isolation and identification from methanol extract of Euonymus alatus. Life Sci. 2005; 77(22): 2760-2769. [DOI:10.1016/j.lfs.2005.02.028]
19. Santana-Gálvez J, Villela Castrejón J, Serna-Saldívar SO and Jacobo-Velázquez DA. Anticancer potential of dihydrocaffeic acid: a chlorogenic acid metabolite. CYTA J. Food. 2020; 18(1): 245-248. [DOI:10.1080/19476337.2020.1743762]
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