year 21, Issue 83 (9-2022)                   J. Med. Plants 2022, 21(83): 11-18 | Back to browse issues page

Research code: 455962
Ethics code: IR.BUMS.REC.1399.078

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Bashiri Z, Yousefi M, Royce S G, Fereidouni M. Antibacterial activity of aqueous and lipid extracts of five common allergenic pollens. J. Med. Plants 2022; 21 (83) :11-18
1- Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran
2- Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
3- Department of Pharmacology, Monash University, Clayton, Australia
4- Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran ,
Abstract:   (1758 Views)
Background: Different parts of plants are rich sources of bioactive ingredients and have received much attention during recent decades. Objective: The aim of this study was to assess the antibacterial properties of five common allergenic pollens extracts. Methods: The aqueous and lipid extracts were prepared from allergenic plant pollens including Amaranthus retroflexus, Chenopodium album, Artemisia vulgaris, Cupressus arizonica, and Ailanthus altissima. Antibacterial activity of the extracts was determined using the broth microdilution method against ATCC bacteria strains. Results: Total protein content of aqueous extracts was form 453 to 2772 μg/ml, but for all lipid extracts it was less than 0.2 μg/ml. Aqueous pollen extracts of C. album, C. arizonica and A. altissima (MIC between 38.8 to 562 μg/ml), and the lipid pollen extracts of C. album, C. arizonica, A. altissima, A. retroflexus, and A. vulgaris (MIC between 0.89 to 21.9 μg/ml) had a significant antibacterial activity on E. faecalis. Moreover, the aqueous pollen extract of A. altissima (MIC = 8.7 μg/ml), and the lipid pollen extracts of C. arizonica, A. altissima, A. retroflexus, and A. vulgaris (MIC between 1.78 to 21.9 μg/ml) showed a significant antibacterial activity against S. aureus. The only extract with antibacterial effect on K. pneumoniae was the lipid pollen extract of A. vulgaris (MIC value of 3.72 μg/ml). Conclusion: The results of this study showed the significant antibacterial activity of some plants pollen extracts. More studies are needed to further assess the active compounds in the pollen extracts and their cytotoxic effects.
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Type of Study: Research | Subject: Medicinal Plants
Received: 2022/04/13 | Accepted: 2022/07/12 | Published: 2022/09/28

1. Arquillué CP, Herrera A, Juan M, García M and Juan T. Pollen analysis and antibacterial activity of spanish honeys: Note. Food Sci. Technol. Int. 2001; 7(2): 155-158. [DOI:10.1177/108201301772660330]
2. Johnston M, McBride M, Dahiya D, Owusu-Apenten R and Nigam PS. Antibacterial activity of Manuka honey and its components: An overview. AIMS Microbiol. 2018; 4(4): 655. [DOI:10.3934/microbiol.2018.4.655]
3. Albaridi NA. Antibacterial potency of honey. Int. J. Microbiol. 2019; 2019. [DOI:10.1155/2019/2464507]
4. Qi Z. WHO traditional medicine strategy. 2014-2023. Geneva: World Health Organization. 2013: 188.
5. Ashraf K, Halim H, Lim SM, Ramasamy K and Sultan S. In vitro antioxidant, antimicrobial and antiproliferative studies of four different extracts of Orthosiphon stamineus, Gynura procumbens and Ficus deltoidea. Saudi J. Biol. Sci. 2020; 27(1): 417-432. [DOI:10.1016/j.sjbs.2019.11.003]
6. Didaras NA, Karatasou K, Dimitriou TG, Amoutzias GD and Mossialos D. Antimicrobial activity of bee-collected pollen and beebread: State of the art and future perspectives. Antibiotics 2020; 9(11): 811. [DOI:10.3390/antibiotics9110811]
7. Carpes ST, Begnini R, Alencar SMd and Masson ML. Study of preparations of bee pollen extracts, antioxidant and antibacterial activity. Cienc. Agrotecnologia 2007; 31: 1818-1825. [DOI:10.1590/S1413-70542007000600032]
8. Atsalakis E, Chinou I, Makropoulou M, Karabournioti S and Graikou K. Evaluation of phenolic compounds in Cistus creticus bee pollen from Greece. Antioxidant and antimicrobial properties. Nat. Prod. Commun. 2017; 12(11): 1934578X1701201141. [DOI:10.1177/1934578X1701201141]
9. Bakour M, Laaroussi H, Ousaaid D, Oumokhtar B and Lyoussi B. Antioxidant and Antibacterial Effects of Pollen Extracts on Human Multidrug-Resistant Pathogenic Bacteria. J. Food Qual. 2021; 2021. [DOI:10.1155/2021/5560182]
10. Kačániová M, Terentjeva M, Petrová J, Hleba L, Shevtsova T, Kluz M, Rožek P and Vukovič N. Antimicrobial activity of nontraditional plant pollen against different species of microorganisms. J. Microbiol. Biotechnol. Food Sci. 2021; 2021: 80-82. [DOI:10.15414/jmbfs.2014.4.1.80-82]
11. Collinvaux PA, De Oliveira PE and Moreno E. Amazon: pollen manual and atlas. CRC Press; 2003. [DOI:10.1201/9781482283600]
12. Breil C, Abert Vian M, Zemb T, Kunz W and Chemat F. "Bligh and Dyer" and Folch methods for solid-liquid-liquid extraction of lipids from microorganisms. Comprehension of solvatation mechanisms and towards substitution with alternative solvents. Int. J. Mol. Sci. 2017; 18(4): 708. [DOI:10.3390/ijms18040708]
13. Wayne, PA: Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing; 30th ed. CLSI document M100-S23.; 2020.
14. Silva ACO, Santana EF, Saraiva AM, Coutinho FN, Castro RHA, Pisciottano MNC, Amorim ELC and Albuquerque UP. Which approach is more effective in the selection of plants with antimicrobial activity? Evid. Based Complement Alternat. Med. 2013; 2013. [DOI:10.1155/2013/308980]
15. Le TN, Luong HQ, Li H-P, Chiu C-H and Hsieh P-C. Broccoli (Brassica oleracea L. var. italica) sprouts as the potential food source for bioactive properties: A comprehensive study on in vitro disease models. Foods 2019; 8(11): 532. [DOI:10.3390/foods8110532]
16. Albouchi F, Hassen I, Casabianca H and Hosni K. Phytochemicals, antioxidant, antimicrobial and phytotoxic activities of Ailanthus altissima (Mill.) Swingle leaves. S. Afr. J. Bot. 2013; 87: 164-174. [DOI:10.1016/j.sajb.2013.04.003]
17. Zhao C-C, Shao J-H, Li X, Xu J and Zhang P. Antimicrobial constituents from fruits of Ailanthus altissima SWINGLE. Arch. Pharm. Res. 2005; 28(10): 1147-1151. [DOI:10.1007/BF02972977]
18. Rahman A, Kim EL and Kang SC. Antibacterial and antioxidant properties of Ailanthus altissima Swingle leave extract to reduce foodborne pathogens and spoiling bacteria. J. Food Saf. 2009; 29(4): 499-510. [DOI:10.1111/j.1745-4565.2009.00172.x]
19. Ajaib M, Hussain T, Farooq S and Ashiq M. Analysis of antimicrobial and antioxidant activities of Chenopodium ambrosioides: An ethnomedicinal plant. J. Chem. 2016; 2016. [DOI:10.1155/2016/4827157]
20. Amjad L and Alizad Z. Antibacterial Activity of the Chennopodium album leaves and flowers extract. World Acad. Sci. Eng. Technol. 2012; 61: 903-906. doi: 10.5281/ zenodo.1079244.
21. Marinaş IC, Chifiriuc C, Oprea E and Lazăr V. Antimicrobial and antioxidant activities of alcoholic extracts obtained from vegetative organs of A. retroflexus. Roum. Arch. Microbiol. Immunol. 2014; 73(1-2): 35-42.
22. Addo-Mensah A, Garcia G, Maldonado I, Anaya E, Cadena G and Lee L. Evaluation of antibacterial activity of Artemisia vulgaris extracts. Res. J. Med. Plant 2015; 9(5): 234. [DOI:10.3923/rjmp.2015.234.240]
23. Silici S and Kutluca S. Chemical composition and antibacterial activity of propolis collected by three different races of honeybees in the same region. J. Ethnopharmacol. 2005; 99(1): 69-73. [DOI:10.1016/j.jep.2005.01.046]
24. Velásquez P, Rodríguez K, Retamal M, Giordano Villatoro A, Valenzuela Roediger LM and Montenegro Rizzardini G. Relation between composition, antioxidant and antibacterial activities and botanical origin of multi-floral bee pollen. J. Appl. Bot. Food Qual. 2017; 90: 306-314.

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