year 20, Issue 80 (12-2021)                   J. Med. Plants 2021, 20(80): 23-33 | Back to browse issues page


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Habibzade A, Moshtaghi N, Malekzadeh-Shafaroudi S. Phytochemical investigation among different populations of Papaver bracteatum Lindl. using ultrasonic - assisted extraction method followed by HPLC analysis. J. Med. Plants. 2021; 20 (80) :23-33
URL: http://jmp.ir/article-1-3140-en.html
1- PhD student in Agricultural Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
2- Department of Biotechnology and Plant Breeding, Ferdowsi University of Mashhad, Mashhad, Iran , moshtaghi@um.ac.ir
3- Department of Biotechnology and Plant Breeding, Ferdowsi University of Mashhad, Mashhad, Iran
Abstract:   (147 Views)
Background: Papaver bracteatum Lindl. (Persian poppy) has a very long history as the major source of natural alkaloids. Among Papaver species, Persian poppy can be considered as a rich source of thebaine. Objective: The purpose of this study was to evaluate the thebaine content in different organs and different populations of Persian poppy. Methods: Seven populations of Persian poppy were collected from five different geographical regions of Iran including Damavand (Rineh, Abask), Chalous (Yoush, Pol-e Zanguleh), Mahabad, Meshgin Shahr and Zanjan (Taham) and were screened for its thebaine content. Extraction of thebaine was performed using the ultrasonic-assisted extraction (UAE) method. The parameters including pH, amount of solvent, extraction temperature and time, the repetition of extraction cycles and also the particle size were investigated and optimal conditions were determined to reach the maximum efficiency. Also, the correlation between phenology of the plant and thebaine content was studied during the developmental stages of poppy from pre-flowering to lancing. The developed UAE method followed by high performance liquid chromatography (HPLC) was used for quantitative analysis. Results: Thebaine content was significantly different in various organs and populations grew at different regions. The thebaine content was in the range of 0.9-1.4 % and 0.1-0.45 % in capsules and stems, respectively. The highest thebaine content was obtained in the capsules of Mahabad population. Conclusion: Extraction led to a high purity thebaine from Persian poppy. Eventually, a comprehensive profile of thebaine in different Persian poppy populations from different geographical regions in Iran was released.
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Type of Study: Research | Subject: Medicinal Plants
Received: 2021/06/19 | Accepted: 2021/11/6 | Published: 2021/12/10

References
1. Karimi M and Raofie F. Micronization of vincristine extracted from Catharanthus roseus by expansion of supercritical fluid solution. J. Supercrit Fluid. 2019; 146: 172-179. [DOI:10.1016/j.supflu.2019.01.021]
2. Ma Y, Cheng J, Inanaga S and Shui J. Induction and inhibition of Striga hermonthica (Del.) Benth. germination by extracts of traditional Chinese medicinal herbs. J. Agron. 2004; 96: 1349-1356. [DOI:10.2134/agronj2004.1349]
3. Lavie D, Rotman J, Levy A and Palevitch D. A rapid quantitative method for the determination of thebaine in Papaver bracteatum. Phytochem. 1979; 18: 2011-2013. [DOI:10.1016/S0031-9422(00)82722-3]
4. Foroozandeh-Shahraki A, Mehrafarin A, Akhondzadeh S, Naghdi Badi H, Qaderi A, Hajiaghaee R, Khalighi-Sigaroodi F and Ghorbani Nahooji M. Analytical Review on Persian Poppy (Papaver bracteatum Lindl.) as a Native and Economical Medicinal Plant. J. M. Plants. 2013; 12: 1-12.
5. Qavami N, Azizi M, Yazdian F, Qaderi A and Nemati H. Effective Induction of Hairy Roots in Persian Poppy (Papaver bracteatum Lindl.) Using Sonication Method. J. M. Plants 2018; 17: 73-82.
6. Goldblatt P. Biosystematic studies in Papaver section Oxytona. Pascal and Francis Bibliographic Database 1974; 61: 264-294. [DOI:10.2307/2395056]
7. Meshulam H and Lavie D. The alkaloidal constituents of Papaver bracteatum Arya II. Phytochem. 1980; 19: 2633-2635. [DOI:10.1016/S0031-9422(00)83934-5]
8. Sharghi N and Lalezari I. Papaver bracteatum Lindl., a highly rich source of thebaine. Nature. 1967; 213: 1244-1244. [DOI:10.1038/2131244a0]
9. Jovanov P, Petrin-Miličević M, Radosavljević-Stevanović N, Vraneš M, Belić S, Sakač M, Nikolov J and Gadžurić S. Rapid Determination of the Primary Alkaloids in Illicit Heroin by High-Performance Liquid Chromatography with Tandem Mass Spectrometry (HPLC-MS/MS). Anal. Lett.. 2020; 54: 1224-1232. [DOI:10.1080/00032719.2020.1798454]
10. Paul B. D, Dreka C, Knight E. S and Smith M. L. Gas chromatographic/mass spectrometric detection of narcotine, papaverine, and thebaine in seeds of Papaver somniferum. Planta Med. 1996; 62: 544-547. [DOI:10.1055/s-2006-957966]
11. Barnett N, Hindson B, Lewis S and Purcell S. Determination of codeine, 6-methoxycodeine and thebaine using capillary electrophoresis with tris (2, 2′-bipyridyl) ruthenium (II) chemiluminescence detection. Anal Commun. 1988; 35: 321-324. [DOI:10.1039/a805955a]
12. Valadbeigi Y, Ilbeigi V and Mirsharifi M. S. Mechanism of atmospheric pressure chemical ionization of morphine, codeine, and thebaine in corona discharge -ion mobility spectrometry: Protonation, ammonium attachment, and carbocation formation. J. Mass Spectrom. 2020; 55: 4586. [DOI:10.1002/jms.4586]
13. Agnihotri A, Tewari S. C, Khatod P, Banerjee S and Balasubramanian M. Determination of thebaine in crude thebaine samples by infrared and ultraviolet spectrophotometric methods. Analyst. 1984; 109: 1413-1416. [DOI:10.1039/an9840901413]
14. Williams RD and Ellis B. E. Age and tissue distribution of alkaloids in Papaver somniferum. Phytochem. 1989; 28: 2085-2088. [DOI:10.1016/S0031-9422(00)97924-X]
15. Bar C. Coffman C and Gentner W. Growth, Development, and thebaine Content of Papaver bracteatum Lindl. in Relation to Spacing1. J. Agron. 1987; 79: 935-937. [DOI:10.2134/agronj1987.00021962007900050036x]
16. Fang H, Zeng Z, Liu L and Pang D. On-line back-extraction field-amplified sample injection method for directly analyzing cocaine and thebaine in the extractants by solvent microextraction. Anal. Chem.. 2006; 78: 1257-1263. [DOI:10.1021/ac0516537]
17. Yoshimatsu K. Kiuchi F. Shimomura K and Makino Y. A rapid and reliable solid-phase extraction method for high-performance liquid chromatographic analysis of opium alkaloids from Papaver plants. Chem. Pharm. Bull. 2005; 53: 1446-1450. [DOI:10.1248/cpb.53.1446]
18. Janicot J, Caude M, Rosset R and Veuthey J. Extraction of major alkaloids from poppy straw with near-critical mixtures of carbon dioxide and polar modifiers. J. Chromatogr. A. 1990; 505: 247-256. [DOI:10.1016/S0021-9673(01)93083-3]
19. Seidi S, Yamini Y, Heydari A, Moradi M, Esrafili A and Rezazadeh M. Determination of thebaine in water samples, biological fluids, poppy capsule, and narcotic drugs, using electromembrane extraction followed by high-performance liquid chromatography analysis. Anal. Chim. Acta.. 2011; 701: 181-188. [DOI:10.1016/j.aca.2011.05.042]
20. Fakhari A. R. Nojavan S. Ebrahimi S. N and Evenhuis C. J. Optimized ultrasound‐assisted extraction procedure for the analysis of opium alkaloids in papaver plants by cyclodextrin‐modified capillary electrophoresis. J. Sep. Sci. 2010; 33: 2153-2159. [DOI:10.1002/jssc.201000135]
21. Yazdani D, Rezazadeh Sh and Shahnazi S. Review of poppy (Papaver somniferum L.). J. M. Plants 2003; 2: 1-12.
22. Hofman P and Menary R. Alkaloid losses from the capsules of Papaver somniferum L. during kiln drying and storage under commercial conditions in Tasmania. J. Stored Prod. Res.. 1985; 21: 135-139. [DOI:10.1016/0022-474X(85)90007-4]
23. Rezaei M. Naghavi M. R. Hoseinzade A. H and Abbasi A. Developmental accumulation of thebaine and some gene transcripts in different organs of Papaver bracteatum. Ind. Crops Prod. 2016; 80: 262-268. [DOI:10.1016/j.indcrop.2015.11.009]
24. Qaderi A. Omidi M. Pour-Aboughadareh A. Poczai P. Shaghaghi J. Mehrafarin A and Etminan A. Molecular diversity and phytochemical variability in the Iranian poppy (Papaver bracteatum Lindl.): A baseline for conservation and utilization in future breeding programmers. Ind Crops Prod. 2019; 130: 237-247. [DOI:10.1016/j.indcrop.2018.12.079]
25. Lalezari I. Nasseri P and Asgharian R. Papaver bracteatum lindl: Population arya II. J. Pharm. Sci. 1974; 63: 1331. [DOI:10.1002/jps.2600630844]
26. Rezaei M, Naghavi MR, Hosseinzadeh AH and Abbasi A. Measurement of some benzylisoquinoline alkaloids in different organs of Persian poppy during onto genetical stages. Chem. Biodivers. 2006; 135: 539-543. [DOI:10.1002/cbdv.201500172]

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