year 20, Issue 78 (5-2021)                   J. Med. Plants 2021, 20(78): 59-67 | Back to browse issues page

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Esmaeili S, Asadi M, Mosaddegh M, Mohammadi Motamed S, Hamzeloo-Moghadam M. Cytotoxic activity of Centaurea albonitens Turrill aerial parts in colon and breast cancer cell lines. J. Med. Plants. 2021; 20 (78) :59-67
1- Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
3- Departtment of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4- Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. ,
Abstract:   (171 Views)
Background: Cancer is the second cause of death in developed countries. Colon and breast cancers are among the most prevalent ones. Research focusing on finding new natural products with fewer side effects to fight cancer is increasing. Objectives: The present study aimed to evaluate the cytotoxicity of Centaurea albonitens Turrill methanol extract and its fractions against colon and breast cancer cell lines and a normal cell line of bovine kidney cells. Methods: The methanol extract and petroleum ether, chloroform and aqueous fractions were provided from the aerial parts of C. albonitens by maceration method in three days. Each day the solvent was refreshed. Colon (HT-29) and breast (MCF-7) cell lines were treated with the extract/fractions for 48 h for evaluating the cytotoxic activity by MTT assay. The apoptotic induction potential was also evaluated with the Hoechst 33258 staining method. Results: The most considerable effect was reported from the chloroform fraction with IC50 values of 25.6 and 25.1 μg/mL in MCF-7 and HT-29 cells, respectively. In Hoechst staining, condensed chromatin of the apoptotic cells was observed in both cell lines. Conclusion: Centaurea albonites can be suggested for further cancer research studies.
Full-Text [PDF 451 kb]   (103 Downloads)    
Type of Study: Research | Subject: Pharmacognosy & Pharmaceutics
Received: 2021/02/4 | Accepted: 2021/05/11 | Published: 2021/06/1

1. Jordan CT, Guzman ML and Noble M. Cancer stem cells. N. Engl. J. Med. 2006; 355(12): 1253-61. [DOI:10.1056/NEJMra061808]
2. Hanahan D and Weinberg RA. Hallmarks of cancer: the next generation. Cell. 2011; 144(5): 646-74. [DOI:10.1016/j.cell.2011.02.013]
3. Dean M. ABC transporters, drug resistance, and cancer stem cells. J. Mammary Gland. Biol. Neoplasia. 2009; 14(1):3-9. [DOI:10.1007/s10911-009-9109-9]
4. Safa O, Soltanipoor MA, Rastegar S, Kazemi M, Dehkordi KN and Ghannadi A. An ethnobotanical survey on hormozgan province, Iran. Avicenna. J. Phytomed. 2013; 3(1): 64-81.
5. Tešević V, Milosavljević S, Vajs V, Janaćković P, Đorđević I and Jadranin M. Quantitative analysis of sesquiterpene lactone cnicin in seven Centaurea species wild-growing in Serbia and Montenegro using 1H-NMR spectroscopy. J. Serbian Chem. Soc. 2007; 72(12): 1275-80. [DOI:10.2298/JSC0712275T]
6. Khan AN, Fatima I, Khaliq UA, Malik A, Miana GA and Qureshi Z-u-R. Potent anti-platelet constituents from Centaurea iberica. Molecules 2011; 16(3): 2053-64. [DOI:10.3390/molecules16032053]
7. Bahmani F, Esmaeili S, Bashash D, Gharehbaghian A. Cytotoxicity of three species of Centaurea genus on acute lymphoblastic leukemia cell line (Nalm-6). J. Med. Plants. 2018; 17(66): 156-66.
8. Bahmani F, Esmaeili S, Bashash D, Dehghan-Nayeri N, Mashati P, Gharehbaghian A. Centaurea albonitens extract enhances the therapeutic effects of Vincristine in leukemic cells by inducing apoptosis. Biomed. Pharmacother. 2018; 99: 598-607. [DOI:10.1016/j.biopha.2018.01.101]
9. Mosaddegh M, Gharanjik BM, Naghibi F, Esmaeili S, Pirani A, Eslami Tehrani B, Keramatian B and Hassanpour A. A survey of cytotoxic effects of some marine algae in the Chabahar coast of Oman Sea. Res. J. Pharmacogn. 2014; 1(1): 27-31.
10. Esmaeil S, Ghiaee A, Naghibi F and Mosaddegh M. Antiplasmodial activity and cytotoxicity of plants used in traditional medicine of Iran for the treatment of fever. Iran. J. Pharm. Res. 2015; 14: 103-7.
11. Aghaei M, Karami-Tehrani F, Panjehpour M, Salami S and Fallahian F. Adenosine induces cell-cycle arrest and apoptosis in androgen-dependent and independent prostate cancer cell lines, LNcap-FGC-10, DU-145, and PC3. Prostate. 2012; 72(4): 361-375. [DOI:10.1002/pros.21438]
12. Koukoulitsa E, Skaltsa H, Karioti A, Demetzos C and Dimas K. Bioactive sesquiterpene lactones from Centaurea species and their cytotoxic/cytostatic activity against human cell lines in vitro. Planta Med. 2002; 68(07): 649-52. [DOI:10.1055/s-2002-32893]
13. Shoeb M, Jaspars M, MacManus SM, Celik S, Nahar L and Kong-Thoo-Lin P. Anti-colon cancer potential of phenolic compounds from the aerial parts of Centaurea gigantea (Asteraceae). J. Nat. Med. 2007; 61(2): 164. [DOI:10.1007/s11418-006-0117-4]
14. Shoeb M, MacManus SM, Jaspars M, Trevidu J, Nahar L and Kong-Thoo-Lin P. Montamine, a unique dimeric indole alkaloid, from the seeds of Centaurea montana (Asteraceae), and its in vitro cytotoxic activity against the CaCo2 colon cancer cells. Tetrahedron 2006; 62(48): 11172-7. [DOI:10.1016/j.tet.2006.09.020]
15. El-Najjar N, Dakdouki S, Darwiche N, El-Sabban M, Saliba NA and Gali-Muhtasib H. Anti-colon cancer effects of Salograviolide A isolated from Centaurea ainetensis. Oncol. Rep. 2008; 19(4): 897-904. [DOI:10.3892/or.19.4.897]
16. Shoeb M, Celik S, Jaspars M, Kumarasamy Y, MacManus SM and Nahar L. Isolation, structure elucidation and bioactivity of schischkiniin, a unique indole alkaloid from the seeds of Centaurea schischkinii. Tetrahedron 2005; 61(38): 9001-6. [DOI:10.1016/j.tet.2005.07.047]
17. Sarker SD, Shoeb M, Celik S, Jaspars M, Nahar L and Kong-Thoo-Lin P. Extracts of Centaurea bornmuelleri and Centaurea huber-morathii inhibit the growth of colon cancer cells in vitro. Orient. Pharm. Exp. Med. 2007; 7(4): 336-40. [DOI:10.3742/OPEM.2007.7.4.336]
18. Sen A, Ozbas ST, Akbuga J and Bitis L. In vitro antiproliferative activity of endemic Centaurea kilaea Boiss. against human tumor cell lines. Clin. Exp. Health. Sci. 2015;5(3):149. [DOI:10.5455/musbed.20150602022750]
19. Nasr FA, Shahat AA, Alqahtani AS, Ahmed MZ, Qamar W, Al-Mishari AA and Almoqbil AN. Centaurea bruguierana inhibits cell proliferation, causes cell cycle arrest, and induces apoptosis in human MCF-7 breast carcinoma cells. Mol. Biol. Rep. 2020; 47(8): 6043-6051. [DOI:10.1007/s11033-020-05679-x]
20. Shahrestanaki MK, Bagheri M, Ghanadian M3, Aghaei M and Jafari SM. Centaurea cyanus extracted 13-O-acetylsolstitialin A decrease Bax/Bcl-2 ratio and expression of cyclin D1/Cdk-4 to induce apoptosis and cell cycle arrest in MCF-7 and MDA-MB-231 breast cancer cell lines. J. Cell. Biochem. 2019; 120(10): 18309-18319. [DOI:10.1002/jcb.29141]
21. Bakr RO, Halim Mohamed SA and Ayoub N. phenolic profile of Centaurea aegyptiaca L. growing in Egypt and its cytotoxic and antiviral activities. Afr. J. Tradit. Complement. Altern. Med. 2016 29; 13(6): 135-143. [DOI:10.21010/ajtcam.v13i6.19]
22. Köse YB, Işcan G, Göger F, Akalın G, Demirci B and Başer KHC. Chemical composition and biological activity of Centaurea baseri: new species from Turkey. Chem. Biodivers. 2016; 13(10): 1369-1379. [DOI:10.1002/cbdv.201600070]
23. Ostad SN, Rajabi A, Khademi R, Farjadmand F, Eftekhari M, Hadjiakhoondi A, Khanavi M. Cytotoxic Potential of Centaurea bruguierana ssp. belangerana: the MTT assay. Acta. Med. Iran. 2016; 54(9): 583-89.
24. Sura Baykan Erel, Serdar Demir, Ayse Nalbantsoy, Petek Ballar, Shabana Khan, N. Ulku Karabay Yavasoglu & Canan Karaalp Bioactivity screening of five Centaurea species and in-vivo anti-inflammatory activity of C. athoa. Pharm. Biol. 2014; 52: 775-81. [DOI:10.3109/13880209.2013.868493]
25. Csapi B, Hajdú Z, Zupkó I, Berényi A, Forgo P, Szabó P, Hohmann J. Bioactivity-guided isolation of antiproliferative compounds from Centaurea arenaria. Phytother Res. 2010; 24(11): 1664-9. [DOI:10.1002/ptr.3187]

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