year 9, Issue 34 (5-2010)                   J. Med. Plants 2010, 9(34): 90-94 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Haeri M, Shams-Ardakani M, Rahbani M, Izaddust M, Khanavi M. Quantification of 4-hydroxyisoleucine content in treated and untreated Fenugreek seeds using GC. J. Med. Plants 2010; 9 (34) :90-94
1- Department of Clinical Biochemistry, Faculty of Medicine, Qom University of Medical Sciences
2- Department of Phamacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences ,
3- Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences
4- Department of Phamacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences
Abstract:   (6137 Views)
Background: There are some reports regarding antidiabetic activity of 4-hydroxyisoleucine, however its hypoglycemic property must be proven completely before presentating as a new drug. Preparing suitable amount of 4-hydroxyisoleucine is nesessary for supporting in vivo studies. Objective: The purpose of the present study was isolation of 4-hydroxyisoleucine, comparing 4-hydroxyisoleucine content in two samples of Fenugreek seeds collected form two distinct parts of Iran and finally the effect of germination and supplementation by isoleucine on 4-hydroxyisoleucine yield. Methods: 4-hydroxyisoleucine was successfully isolated passing seed amino acid extract through ion exchange and silica gel column and then crystallization on methanol. 4-hydroxyisoleucine content in various samples was assessed by GC. Results: Seeds from Northwestern of Iran had higher amount of 4-hydroxyisoleucine in comparison to Central Iran. Furthermore yields from germinating seeds were 2 folds higher than the dried seeds, and could be increased 6 fold by inclusion of isoleucine supplement in the irrigation water. Conclusion: simple procedures can be engaged for improving yields of 4-hydroxyisoleucine isolated from fenugreek up to six-fold by using of germinated seeds supplemented with isoleucine.
Full-Text [PDF 206 kb]   (2832 Downloads)    
Type of Study: Research | Subject: Pharmacognosy & Pharmaceutics
Received: 2006/05/15 | Accepted: 2008/01/12 | Published: 2010/06/19

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Journal of Medicinal Plants

Designed & Developed by : Yektaweb