year 19, Issue 76 (11-2020)
J. Med. Plants 2020, 19(76): 36-45 |
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Research code: 1396–1102
Ethics code: IR.MAZUMS.REC1398.113
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Azadbakht M, Davoodi A, Hosseinimehr S J, Emami S, Azadbakht M, Mirzaee F et al . Phytochemical, physicochemical and biological evaluation of Colchicum kurdicum (Bornm.) Stef.: a study on materia medica of Persian medicine. J. Med. Plants 2020; 19 (76) :36-45
URL: http://jmp.ir/article-1-2685-en.html
URL: http://jmp.ir/article-1-2685-en.html
Mohammad Azadbakht1 
, Ali Davoodi * 2, Seyed Jalal Hosseinimehr3 , Saeed Emami4 , Masoud Azadbakht5 , Fatemeh Mirzaee1 , Hossein Bakhshi Jouybari1
, Ali Davoodi * 2, Seyed Jalal Hosseinimehr3 , Saeed Emami4 , Masoud Azadbakht5 , Fatemeh Mirzaee1 , Hossein Bakhshi Jouybari1
1- Department of Pharmacognosy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
2- Department of Pharmacognosy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran , adavoodi.pharm@gmail.com
3- Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
4- Department of Medicinal Chemistry and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
5- Department of Plant Systematics, High Educational of Sanna Institute, Sari, Iran
2- Department of Pharmacognosy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran , adavoodi.pharm@gmail.com
3- Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
4- Department of Medicinal Chemistry and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
5- Department of Plant Systematics, High Educational of Sanna Institute, Sari, Iran
Abstract: (3174 Views)
Background: The genus Colchicum (Colchicaceae) is a perennial and monocotyledon flowering plant that has more than 160 species in the world. In addition, this plant is an important medicinal plant in traditional and modern medicines. Objective: The aim of this study was to evaluate the phytochemical profiles and physicochemical properties, antioxidant and anti-inflammatory activities of Colchicum kurdicum (Bornm.) Stef. corm. Methods: Phytochemical profiles includedtotal tropolone alkaloid, total phenolic/total tannin and total flavonoid contents were determined by spectrophotometric method. Moreover, tropolone alkaloid profiles was analyzed by HPLC method. Physicochemical properties including macroscopic and organoleptic properties, solubility, foreign matter, ash values and heavy metals were evaluated based on pharmacopeial protocoles. In addition, in vitro anti-inflammatory and antioxidant activities of the plant were determined. Results: Total tropolone alkaloids, phenol, tannin and flavonoid contents of the corm were estimated to be 0.652, 0.426, 0.052 and 0.325 g/100 g corm. In HPLC analysis, N-deacetyl-N-formyl colchicine, colchifoline, colchicoside and cornigerine were calculated as highest bioactive tropolone alkaloids. Physicochemical properties were determined within an acceptable range for the pharmacopoeia. Moreover, the anti-inflammatory and antioxidant activities in 10 mg/ml of methanolic extract were estimated 84.7 % and 94.8 %, respectively. Conclusion: The corm of the Colchicum kurdicum had the main bioactive compounds. In addition, these compounds have the valuable biological activities as anti-inflammatory and antioxidant activities.
Keywords: Colchicum kurdicum , Tropolone alkaloid , Total flavonoid , Chromatography , Antioxidant , Anti-inflammatory activity
Type of Study: Research |
Subject:
Pharmacognosy & Pharmaceutics
Received: 2019/10/13 | Accepted: 2020/07/19 | Published: 2020/12/1
Received: 2019/10/13 | Accepted: 2020/07/19 | Published: 2020/12/1
References
1. Gulsoy-Toplan G, Goger F, Yildiz-Pekoz A, Gibbons S, Sariyar G and Mat A. Chemical constituents of the different parts of Colchicum micranthum and C. chalcedonicum and their cytotoxic activities. Nat. Pro. Commun. 2018; 13(5): 1-5. [DOI:10.1177/1934578X1801300506]
2. Alali FQ, El-Alali A, Tawaha K and El-Elimat T. Seasonal variation of colchicine content in Colchicum brachyphyllum and Colchicum tunicatum (Colchicaceae). Nat. Prod. Res. 2006; 20(12): 1121-8. [DOI:10.1080/14786410600857504]
3. Al-Fayyad M, Alali F, Alkofahi A and Tell A. Determination of colchicine content in Colchicum hierosolymitanum and Colchicum tunicatum under cultivation. Nat. Prod. Let. 2002; 16(6): 395-400. [DOI:10.1080/10575630290033178]
4. Khan H, Tariq SA and Khan MA. Biological and phytochemical studies on corms of Colchicum luteum Baker. J. Med. Plant. Res. 2011; 5(32): 7031-5. [DOI:10.5897/JMPR11.1159]
5. Shafiee F, Khoshvishkaie E, Davoodi A, Dashti Kalantar A, Bakhshi Jouybari H and Ataee R. The determination of blood glucose lowering and metabolic effects of Mespilus germanica L. hydroacetonic extract on Streptozocin-induced diabetic BALB/c mice. Medicines 2018; 5(1): 1-7. [DOI:10.3390/medicines5010001]
6. Davoodi A, Jouybari HB, Fathi H and Ebrahimnejad P. Formulation and physicochemical evaluation of medlar (Mespilus germanica L.) and oak (Quercus castaneifolia C.A.Mey.) Effervescent tablet. Inter. J. Pharmaceut. Sci. Res. 2018; 9(9): 3870-5.
7. Fakhri M, Davoodi A, Parviz M, Sadeghi Ghadi Z, Mousavinasab SN, Farhadi R, Azadbakht M and Azadbakht M. Characterization and HPLC analysis of manna from some cotoneaster species. Int. J. Pharma. Sci. Res. 2017; 8: 1000-06.
8. Pramparo M, Gregory S and Mattea M. Immersion vs. percolation in the extraction of oil from oleaginous seeds. J. Amer. Oil Chem. Soc. 2002; 79(10):955-60. [DOI:10.1007/s11746-002-0586-3]
9. Becer E, Hanoğlu DY, Kabadayı H, Hanoğlu A, Vatansever S, Yavuz DÖ, et al. The effect of Colchicum pusillum in human colon cancer cells via Wnt/β-catenin pathway. Gene. 2019; 686: 213-9. [DOI:10.1016/j.gene.2018.11.047]
10. Bayrak S, Sökmen M, Aytaç E and Sökmen A. Conventional and supercritical fluid extraction (SFE) of colchicine from Colchicum speciosum. Ind. Crop. Prod. 2019; 128: 80-4. [DOI:10.1016/j.indcrop.2018.10.060]
11. Ondra P, Válka I, Vičar J, Sütlüpinar N and Šimánek V. Chromatographic determination of constituents of the genus Colchicum (Liliaceae). J. Chrom. A. 1995; 704(2): 351-6. [DOI:10.1016/0021-9673(95)00185-P]
12. Organization WH. Quality control methods for medicinal plant materials. WHO Library Cataloguing in Publication Data. 1998; 1: 1-123.
13. Shokrzadeh M, Fathalinezhad F and Khoshvishkaie E. Concentrations of heavy metals (Cr, Cd, Pb) in three types of rice in Astaneh-Ashrafieh, Iran. J. Maz. Univ. Med. Sci. 2017; 26(146): 196-200.
14. Rahman H, Eswaraiah MC and Dutta A. In-vitro anti-inflammatory and anti-arthritic activity of Oryza sativa var. Joha rice (an aromatic indigenous rice of Assam). Am. Eurasian J. Agric. Environ. Sci. 2015; 15: 115-21.
15. Sharma OP and Bhat TK. DPPH antioxidant assay revisited. Food Chem. 2009; 113(4): 1202-5. [DOI:10.1016/j.foodchem.2008.08.008]
16. Senthilkumar M. Phytochemical screening and antibacterial activity of Gloriosa superba Linn. Inter. J. Pharmacogn. Phytochem. Res. 2013; 5(1): 31-6.
17. Pırıldar S, Sütlüpınar N, Atasever B, Erdem-Kuruca S, Papouskova B and Šimánek V. Chemical constituents of the different parts of Colchicum baytopiorum and their cytotoxic activities on K562 and HL60 cell lines. Pharmaceut. Biol. 2010; 48(1): 32-9. [DOI:10.3109/13880200903029373]
18. Suica-Bunghez I-R, Ion R-M, Teodorescu S, Sorescu A-A, Stirbescu R-M and Stirbescu N-M. Fitochemical and antioxidant characterization of autumn crocus (Colchicum autumnale) flowers and roots plant extracts. J. Sci. Art. 2017; 17(3): 539-46.
19. Sangster AW and Stuart KL. Ultraviolet spectra of alkaloids. Chem. Rev. 1965; 65(1): 69-130. [DOI:10.1021/cr60233a003]
20. Ebrahimzadeh MA, Enayatifard R, Khalili M, Ghaffarloo M, Saeedi M and Charati JY. Correlation between sun protection factor and antioxidant activity, phenol and flavonoid contents of some medicinal plants. IJPR. 2014; 13(3): 1041-7.
21. Ahmed SN, Ahmad M, Shinwari ZK and Shinwari S. Taxonomic, pharmacognostic and physicochemical authentication of Colchicum luteum baker (suranjantalkh) from its commercial adulterant. Pak. J. Bot. 2016; 48(5): 2039-45.
22. Silva RJ, de Aguiar-Dias AC, do CF Faial K and de Mendonca MS. Morphoanatomical and physicochemical profile of Piper callosum: valuable assessment for its quality control. Rev. Brasi. Farmacogn. 2017; 27(1): 20-33. [DOI:10.1016/j.bjp.2016.07.006]
23. Mohamad TAST, Naz H, Jalal RS, Hussin K, Rahman MRA, Adam A and Weber JFF. Chemical and pharmacognostical characterization of two Malaysian plants both known as Ajisamat. Rev. Brasil. Farmacogn. 2013; 23(5): 724-30. [DOI:10.1590/S0102-695X2013000500002]
24. Yazdinezhad A, Ramezanloo N and Mozaffari S. Pharmacognostic and phytochemical investigation of Heracleum persicum Desf. ex Fischer. Res. J. Pharmacogn. 2016; 3(2): 17-24.
25. Tüzen M. Determination of heavy metals in fish samples of the middle Black Sea (Turkey) by graphite furnace atomic absorption spectrometry. Food Chem. 2003; 80(1): 119-23. [DOI:10.1016/S0308-8146(02)00264-9]
26. Sivaperumal P, Sankar T and Nair PV. Heavy metal concentrations in fish, shellfish and fish products from internal markets of India vis-a-vis international standards. Food Chem. 2007; 102(3):612-20. [DOI:10.1016/j.foodchem.2006.05.041]
27. Williams LAD, O'Connar A, Latore L, Dennis O, Ringer S, Whittaker JA, Conrad J, Volger B, Rosner H and Kraus W. The in vitro anti-denaturation effects induced by natural products and non-steroidal compounds in heat treated (immunogenic) bovine serum albumin is proposed as a screening assay for the detection of anti-inflammatory compounds, without the use of animals, in the early stages of the drug discovery process. West Ind. Med. J. 2008; 57(4): 327-31.
28. Ramalingam R, Madhavi BB, Nath AR, Duganath N, Sri EU and Banji D. In-vitro anti-denaturation and antibacterial activities of Zizyphus oenoplia. Pharm. Lett. 2010; 2(1): 87-93.
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