year 19, Issue 74 (6-2020)                   J. Med. Plants 2020, 19(74): 118-128 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Nikzad M, Mirdar S. Inhibitory effects of Nigella sativa nano-capsule on the expression of cyclin D1 in the lungs of wistar rats exposed to nicotine-derived nitrosamine ketone. J. Med. Plants 2020; 19 (74) :118-128
URL: http://jmp.ir/article-1-2385-en.html
1- Department of Mathematics, University of Science and Technology of Mazandaran, Iran , mnikzad260@gmail.com
2- Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Mazandaran, Babolsar, Iran
Abstract:   (2662 Views)
Background: Researchers argue that supplements are somewhat capable of influencing the lung tissue exposed to smoking carcinogens through cell cycle. Cyclin D1 is a gene involved in cell cycle. Objective: This study aimed to investigate the effects of 12-week injection of Nigella sativa nano-capsule on the expression of Cyclin D1 of the lung tissue of the rats receiving exposure to Nicotine-Derived Nitrosamine Ketone. Method: 46 Wistar rats were assigned into five groups: NNK, Supplement, and Supplement, + NNK, Control and Vehicle group. 125 μg/kg body weight of Nigella sativa nano-capsule supplement was injected subcutaneously into the Supplement and Supplement NNK groups once a week. NKK and Vehicle groups received a weekly dose of 12.5 mg/kg NNK and distilled water for 12 weeks through subcutaneous injection. After isolating the lung tissue, the expression of the Cyclin D1 gene was measured using Real Time PCR-ABI. One-way ANOVA parametric analysis and Tukey post hoc test were used to analyze the data at the significant level of P ≤ 0.05. Results: According to the results, the expression of Cyclin 1 gene was relatively reduced in Supplement NNK (P = 0.003) and Vehicle (P = 0.001) groups in comparison to the NNK group. Nevertheless, no significant difference was observed among the Supplement, Vehicle, and Control groups. We believe that Nigella sativa nano-capsule supplement reduced the relative changes of Cyclin 1 in the lung tissues exposed to carcinogen NNK. Conclusion: Apparently, this supplement is capable of reducing Cyclin 1 level in cell cycle; hence, it can reduce the carcinogenic effects of NNK and the negative effects of smoking among other therapeutic methods.
Full-Text [PDF 765 kb]   (1138 Downloads)    
Type of Study: Research | Subject: Medicinal Plants
Received: 2018/12/6 | Accepted: 2019/02/18 | Published: 2020/07/21

References
1. Jemal A and et al. Global cancer statistics. CA: A Cancer J. Clinicians 2011; 61 (2): 69-90. [DOI:10.3322/caac.20107]
2. Vargas, AJ and CC. Harris, Biomarker development in the precision medicine era: lung cancer as a case study. Nature Reviews Cancer. 2016; 16(8): 525-37. [DOI:10.1038/nrc.2016.56]
3. Miller A and et al. Differential involvement of gp130 signalling pathways in modulating tobacco carcinogen-induced lung tumourigenesis. Oncogene 2015; 34(12): 1510-9. [DOI:10.1038/onc.2014.99]
4. Ge G.-Z, T.-R. Xu and C. Chen. Tobacco carcinogen NNK-induced lung cancer animal models and associated carcinogenic mechanisms. ABBS. 2015; 47(7): 477-87. [DOI:10.1093/abbs/gmv041]
5. Moses C and et al. Hallmarks of cancer: the CRISPR generation. European Journal of Cancer 2018; 93: 10-8. [DOI:10.1016/j.ejca.2018.01.002]
6. Fu M and et al. Minireview: Cyclin D1: normal and abnormal functions. Endocrinol. 2004; 145(12): 5439-47. [DOI:10.1210/en.2004-0959]
7. Burandt E and et al. Cyclin D1 gene amplification is highly homogeneous in breast cancer. Breast Cancer 2016; 23(1): 111-9. [DOI:10.1007/s12282-014-0538-y]
8. Koka PS and et al. Studies on molecular mechanisms of growth inhibitory effects of thymoquinone against prostate cancer cells: role of reactive oxygen species. Experimental Biology and Medicine 2010; 235(6): 751-60. [DOI:10.1258/ebm.2010.009369]
9. Sayed AAR and M Morcos. Thymoquinone decreases AGE induced NF B activation in proximal tubular epithelial cells. Phytotherapy Res. 2007; 21(9): 898-9. [DOI:10.1002/ptr.2177]
10. Li F, Rajendran P and Sethi G. Thymoquinone inhibits proliferation, induces apoptosis and chemosensitizes human multiple myeloma cells through suppression of signal transducer and activator of transcription 3 activation pathway. British J. Pharmacol. 2010; 161(3): 541-54. [DOI:10.1111/j.1476-5381.2010.00874.x]
11. Attoub S and et al. Thymoquinone as an anticancer agent: evidence from inhibition of cancer cells viability and invasion in vitro and tumor growth in vivo. Fundamental & Clinical Pharmacol. 2013; 27(5): 557-69. [DOI:10.1111/j.1472-8206.2012.01056.x]
12. Roepke M and et al. Lack of p53 augments thymoquinone-induced apoptosis and caspase activation in human osteosarcoma cells. Cancer Biology & Therapy 2007; 6(2): 160-9. [DOI:10.4161/cbt.6.2.3575]
13. Sethi G, Ahn KS and Aggarwal BB. Targeting nuclear factor- B activation pathway by thymoquinone: role in suppression of antiapoptotic gene products and enhancement of apoptosis. Molecular Cancer Res. 2008; 6(6): 1059-70. [DOI:10.1158/1541-7786.MCR-07-2088]
14. Lao Y and et al. Formation and accumulation of pyridyloxobutyl DNA adducts in F344 rats chronically treated with 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone and enantiomers of its metabolite, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol. Chemical Research in Toxicol. 2007; 20(2): 235-45. [DOI:10.1021/tx060207r]
15. Maertens LA and et al. Formation and distribution of NNK metabolites in an isolated perfused rat lung. Drug Metabolism and Disposition 2010; 38(5): 752-60. [DOI:10.1124/dmd.109.031492]
16. Manju S and et al. Antibacterial, antibiofilm and cytotoxic effects of Nigella sativa essential oil coated gold nanoparticles. Microbial Pathogenesis 2016; 91: 129-35. [DOI:10.1016/j.micpath.2015.11.021]
17. Burits M and Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytotherapy Res. 2000; 14(5): 323-8. https://doi.org/10.1002/1099-1573(200008)14:5<323::AID-PTR621>3.0.CO;2-Q [DOI:10.1002/1099-1573(200008)14:53.0.CO;2-Q]
18. Al-Ghamdi MS. Protective effect of Nigella sativa seeds against carbon tetrachloride-induced liver damage. The American J. Chinese Medicine 2003; 31(05): 721-8. [DOI:10.1142/S0192415X03001399]
19. Maryam K, Shakeri S and Kiani K. Preparation andin vitroinvestigation of antigastric cancer activities of carvacrol-loaded human serum albumin nanoparticles. IET Nanobiotechnol. 2015. 9(5): 294-9. [DOI:10.1049/iet-nbt.2014.0040]
20. Zhao L and Wang W. miR-125b suppresses the proliferation of hepatocellular carcinoma cells by targeting Sirtuin7. International J. Clinical and Experimental Medicine 2015; 8(10): 18469.
21. Sayers EW and et al. GenBank. Nucleic Acids Res. 2018; 47 (D1): D94-D99. [DOI:10.1093/nar/gky989]
22. Khazaei M and Pazhouhi M. Temozolomide-Mediated Apoptotic Death Is Improved by Thymoquinone in U87MG Cell Line. Cancer Investigation 2017; 35(4): 225-36. [DOI:10.1080/07357907.2017.1289383]
23. Kaseb, AO and et al. Androgen receptor-and E2F-1-targeted thymoquinone therapy for hormone-refractory prostate cancer. Cancer Res. 2007; 67(16): 7782-8. [DOI:10.1158/0008-5472.CAN-07-1483]
24. Gali-Muhtasib HU and et al. Molecular pathway for thymoquinone-induced cell-cycle arrest and apoptosis in neoplastic keratinocytes. Anti-Cancer Drugs 2004; 15(4): 389-99. [DOI:10.1097/00001813-200404000-00012]
25. Schneider-Stock R and et al. Thymoquinone: fifty years of success in the battle against cancer models. Drug Discovery Today 2014; 19(1): 18-30. [DOI:10.1016/j.drudis.2013.08.021]
26. Ohtani T and et al. Dissection of signaling cascades through gp130 in vivo: reciprocal roles for STAT3-and SHP2-mediated signals in immune responses. Immunity 2000; 12(1): 95-105. [DOI:10.1016/S1074-7613(00)80162-4]
27. Dergarabetian E and et al. Thymoquinone induces apoptosis in malignant T-cells via generation of ROS. Front Biosci (Elite Ed). 2013, 5: p: 706-19. [DOI:10.2741/E651]
28. Wang JD and Levin PA. Metabolism, cell growth and the bacterial cell cycle. Nature Reviews Microbiol. 2009; 7(11): 822-7. [DOI:10.1038/nrmicro2202]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Medicinal Plants

Designed & Developed by : Yektaweb