year 19, Issue 74 (6-2020)
J. Med. Plants 2020, 19(74): 63-72 |
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Shariatzadeh S M, Soleimani Mehranjani M, Taghipour S, Azimi A S. Effect of green tea (Camellia sinensis (L.) Kuntze) extract on sperm health parameters in adult rats treated with Bisphenol A. J. Med. Plants 2020; 19 (74) :63-72
URL: http://jmp.ir/article-1-1807-en.html
URL: http://jmp.ir/article-1-1807-en.html
Seyed Mohammadali Shariatzadeh1 
, Malek Soleimani Mehranjani1 , Samane Taghipour * 2, Atena Sadat Azimi1
, Malek Soleimani Mehranjani1 , Samane Taghipour * 2, Atena Sadat Azimi1
1- Department of Biolog, Arak University, Arak, Iran
2- Department of Biolog, Arak University, Arak, Iran , Samanetaghipor@yahoo.com
2- Department of Biolog, Arak University, Arak, Iran , Samanetaghipor@yahoo.com
Abstract: (2785 Views)
Background: Bisphenol A (BPA) disturbs male reproductive system performance via the production of free radicals. Green tea extract (GTE) is known as a potent antioxidant. Objective: The aim of this study was to investigate the effect of co-treatment of BPA and GTE as an antioxidant on male reproductive system performance. Methods: Adult male rats were divided into 4 groups: control, Bisphenol A (20 µg/kg/day), GTE (100 mg/kg/day) and Bisphenol A + GTE, treated for 8 weeks. The body weight and left testis weight were recorded. Left caudal epididymis was separated and kept in Ham's F10. Released sperms were used for analyzing the number, motility, viability and abnormalities of the sperm. The quality of sperm chromatin was assessed by nuclear staining acridine orange and aniline blue. Data were analyzed using one-way ANOVA and Tukey‘s test. The means were considered significant at P < 0.05. Results: A significant reduction in the number, motility and viability of sperm were seen in BPA group compared to the control group (P < 0.001). BPA didn’t have any effect on sperm DNA integrity and histon-protamine replacement (P > 0.05). Also a considerable increase in sperm viability and motility was seen in the GTE group compared to the control. Conclusion: The results of this investigation showed that GTE could compensate the adverse effects of BPA on sperm parameters in adult rats.
Type of Study: Research |
Subject:
Medicinal Plants
Received: 2017/08/7 | Accepted: 2018/11/20 | Published: 2020/07/21
Received: 2017/08/7 | Accepted: 2018/11/20 | Published: 2020/07/21
References
1. Lyons G. Bisphenol A a Known Endocrine Disruptor. 1st ed. A WWF European Toxics Programme Report. 2000, pp: 21-25.
2. Brotons JA, Olea-Serrano MF, Villalobos M, Pedraza V and Olea N. Xenoestrogens released from lacquer coatings in food cans. Environ Health Perspect 1995; 103 (6): 608-612. [DOI:10.1289/ehp.95103608]
3. Sullivan JB and Krieger GR. Clinical Environmental Health and Toxic Exposures. 2nd ed. Philadelphia. Pennsylvania: Lippincott Williams & Wilkins. 2001, pp: 362-372.
4. Olea N, Pulgar R, Perez P, Olea-Serrano F, Rivas A, Novillo-Fertrell A and et al. Estrogenicity of resin-based composites and sealants used in dentistry. Environ Health Perspect 1996; 104 (3): 298-305. [DOI:10.1289/ehp.96104298]
5. Bolton NJ, Tapanainen J, Koivisto M and Vihko R. Circulating sex hormone-binding globulin and testosterone in newborns and infants. Clin. Endocrinol. 1989; 31 (2): 201-207. [DOI:10.1111/j.1365-2265.1989.tb01243.x]
6. Pastore RL and Fratellone P. Potential Health Benefits of Green Tea (Camellia sinensis): A Narrative Review. Explore. 2006; 2 (6): 531-537. [DOI:10.1016/j.explore.2006.08.008]
7. Gupta J, Siddique YH, Beg T, Ara G and Afzal M. Protective role of green tea extract against genotoxic damage induced by anabolic steroid in cultured human lymphocytes. Biol. Med. 2009; 1 (2): 87-99.
8. Frei, B. and J. V. Higdon. Antioxidant activity of tea polyphenols in vivo: Evidence fromanimal studies. J. Nutr. 2003; 133: 3275S-3284S. [DOI:10.1093/jn/133.10.3275S]
9. Freitas F, Cordeiro-Mori F, Sasso-Cerri E, Lucas S and Miraglia S. Alterations of spermatogenesis in etoposide-treated rats: a stereological study. Interciencia. 2002; 27: 227-235.
10. WHO laboratory manual for the examination and processing of human semen. 5th ed. World Health Organization. 2010, 32: 61-97.
11. Tejada RI, Mitchell JC, Norman A and Marik JJ. A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence. Fertil Steril. 1984; 42: 87-91. [DOI:10.1016/S0015-0282(16)47963-X]
12. Wong A, Chuan SS, Patton WC and Jacobson JD. Addition of eosin to the aniline blue assay to enhance detection of immature sperm histones. Fertil. Steril. 2008; 90: 1999-2002. [DOI:10.1016/j.fertnstert.2007.09.026]
13. Herath CB, Jin W, Watanabe G, Arai K, Suzuki AK and Taya K. Adverse effects of environmental toxicants, octylphenol and bisphenol A, on male reproductive functions in pubertal rats. Endocrine. 2004; 25: 163-172. [DOI:10.1385/ENDO:25:2:163]
14. Bansal A and Bilaspuri G. Impacts of oxidative stress and antioxidants on semen Functions. Vet. Med. Int. 2011; 1-7. [DOI:10.4061/2011/686137]
15. Aikawa H, Koyama S, Matsuda M, Nakahashi K, Akazome Y and Mori T. Relief effect of vitamin A on the decreased motility of sperm and the increased incidence of malformed sperm in mice exposed neonatally to bisphenol A. Cell Tissue Res. 2004; 315 (1): 119-24. [DOI:10.1007/s00441-003-0806-1]
16. Sakaue M, Ohsako S, Ishimura R, Kurosawa S, Kurohmaru M, Hayashi Y, Aoki Y, Yonemoto J and Tohyama C. Bisphenol-A affects spermatogenesis in the adult rat even at a low dose. J. Occup. Health. 2001; 43: 185-190. [DOI:10.1539/joh.43.185]
17. Pengpeng J, Xiaoli W, Fei C, Yinyang B, Yingchun L, Rong Z, and Ling C. Low dose bisphenol A impairs spermatogenesis by suppressing reproductive hormone production and promoting germ cell apoptosis in adult rats. J. Biomed. Res. 2013; 27 (2): 135-144.
18. Ogura RA., Ikeda N, Yuki K, Morita O, Saigo K, Blackstock C, Nishiyama N and Kasamatsu T. Genotoxicity studies on green tea catechin. Food Chem. Toxicol. 2008; 46: 2190-2200. [DOI:10.1016/j.fct.2008.02.016]
19. Evans NP, North T, Dye S and Sweeney T. Differential effects of the endocrine-disrupting compounds bisphenol-A and octylphenol on gonadotropin secretion, in prepubertal ewe lambs. Domest. Anim. Endocrinol. 2004; 26 (1): 61-73. [DOI:10.1016/j.domaniend.2003.09.005]
20. Sato K, Sueoka K, Tanigaki R, Tajima H, Nakabayashi A, Yoshimura Y and Hosoi Y. Green tea extracts attenuate doxorubicin-induced spermatogenic disorders in conjunction with higher telomerase activity in mice. J. Assist. Reprod. Genet. 2010; 27: 501-508. [DOI:10.1007/s10815-010-9438-z]
21. Huszar G, Ozenci CC, Cayli S, Zavaczki Z and et al. Hyaluronic acid binding by human sperm indicates cellular maturity, viability, and unreacted acrosomal status. Fertil. Steril. 2003; 79 (3): 1616-1624. [DOI:10.1016/S0015-0282(03)00402-3]
22. Kazerooni T, Asadi N, Jadid L, Kazerooni M, et al. Evaluation of sperm's chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion. J. Assist. Reprod. Genet. 2009; 26: 591-596. [DOI:10.1007/s10815-009-9361-3]
23. Takao T, Nanamiya W, Nagano I, Asaba K, Kawabata K and Hashimoto K. Exposure with the environmental estrogen bisphenol A disrupts the male reproductive tract in young mice. Life Sci. 1999; 65 (22): 2351-2357. [DOI:10.1016/S0024-3205(99)00502-0]
24. Liu C, Duan W, Li R, Xu S, Zhang L, Chen C, He M, Y Lu, Wu H, Pi H, Luo X, Zhang Y, Zhong M, Yu Z and Z Zhou. Exposure to bisphenol A disrupts meiotic progression during spermatogenesis in adult rats through estrogen-like activity. Cell Death Dis. 2013; 4 (6): 1-9. [DOI:10.1038/cddis.2013.203]
25. Ashby J, Tinwell H, Lefevre PA, Joiner R and Haseman J. The effect on sperm production in adult Sprague-Dawley rats exposed by gavage to bisphenol A between postnatal days 91-97. Toxicol. Sci. 2003; 74 (1): 129-38. [DOI:10.1093/toxsci/kfg093]
26. Takahashi O and Oishi S. Testicular toxicity of dietary 2, 2-bis (4-hydroxyphenyl) propane (bisphenol A) in F344 rats. Arch. Toxicol. 2001; 75 (1): 42-51. [DOI:10.1007/s002040000204]
27. Waechter JM, Dimond SS, Breslin WJ, Butala JH, Cagen SZ, Jekat FW and et al. Evaluation of reproductive organ development in CE-1 mice after prenatal exposure to bisphenol A. Toxicologist. 1999; 48 (1-S): 45-50. [DOI:10.1093/toxsci/50.1.36]
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