year 11, Issue 42 (5-2012)                   J. Med. Plants 2012, 11(42): 154-162 | Back to browse issues page

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Ghazanfari T, Zamani Taghizadeh Rabe S, Yaraee R, Mahmoudi M. Study on Anti-cancer Effect of Plant Extract ACA-1 on Gastric Adenocarcinoma Cell Line. J. Med. Plants 2012; 11 (42) :154-162
1- Department of Immunology, Faculty of Medicine, Shahed University
2- Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences
3- Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences ,
Abstract:   (6597 Views)
Background: Apoptosis provides solutions for the treatment of cancers and its induction is used as a strategy for preparing drugs that destroy pre-neoplastic cells. Many plant compounds have anti-tumoral activity. ACA-1 plant product is an aqueous extract and has been used in traditional medicine in Iran and has cytotoxicity effect on melanoma cancer cells. Objective: In this study, anti-cancer effect of ACA-1 plant product on gastric adenocarcinoma cells was evaluated and the mechanism of its action was studied. Methods: Cytotoxicity of ACA-1 on gastric adenocarcinoma cells (AGS) and fibroblasts (HgF) was determined after 24 hours of incubation by MTT assay. Annexin V-FITC and PI staining method was used for measuring the cell apoptosis. Activity of aspase 8 and 9 was assayed by enzymatic method. An ECMatrix was used for determining invasion ability of AGS cells. Results: ACA-1 showed strong and dose - dependent toxicity on AGS cells by induction of early apoptosis. Increase in caspase 8 and 9 activities was involved in this process. Also, ACA-1 decrease the invasion ability on AGS cells. Conclusion: ACA-1 induced apoptosis in human gastric cancer cells by activating caspase 8 and 9. With respect to decreasing cell invasion of AGS cells, ACA-1 may be considered as a potential candidate against human gastric cancer.
Keywords: Cancer, ACA-1, Apoptosis, Caspase
Full-Text [PDF 405 kb]   (2720 Downloads)    
Type of Study: Research | Subject: Pharmacology & Toxicology
Received: 2010/04/8 | Accepted: 2012/02/15 | Published: 2012/04/19

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