year 25, Issue 97 (5-2026)
J. Med. Plants 2026, 25(97): 69-83 |
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Tavakkoli-Bardaskan M, Asili J, Nejad Ebrahimi S, Boozari M. Optimization of the extraction of total phenols and flavonoids from Scutellaria litwinowii: A green extraction technique based on response surface methodology and macroporous Resins. J. Med. Plants 2026; 25 (97) :69-83
URL: http://jmp.ir/article-1-3895-en.html
URL: http://jmp.ir/article-1-3895-en.html
1- Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran
2- Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
3- Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, IranOrcid: 0000-0003-2167-8032
4- Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran ,bouzarim@mums.ac.ir
2- Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
3- Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, IranOrcid: 0000-0003-2167-8032
4- Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran ,
Abstract: (11 Views)
Background: The flavonoid-rich roots of Scutellaria litwinowii have therapeutic potential, but traditional extraction methods are inefficient. This study developed an optimized protocol using modern extraction techniques to maximize extract yield. Objective: The roots of S. litwinowii contain valuable flavonoid compounds and exhibit biological properties. Response surface methodology and macroporous resins were employed to increase the extraction yield. Methods: Extraction of the plant roots was conducted according to a design optimized with Design-Expert® software. A comparative analysis was performed between reflux-assisted extraction (RAE), microwave-assisted extraction (MAE), and traditional maceration. Subsequently, phenolic and flavonoid compounds were enriched using a Diaion HP-20 resin column. Results: MAE under optimized parameters yielded a higher total phenolic content compared to conventional 72-hour maceration. Moreover, both MAE and RAE demonstrated significantly higher total flavonoid yields than the maceration method. Furthermore, the Diaion HP-20 resin column increased the amount of active compounds in the S. litwinowii extract by approximately 1.75 times. Conclusion: This study employed two principal strategies for the enrichment of phenolic and flavonoid compounds. Firstly, RAE and MAE demonstrated significantly higher efficiency compared to traditional maceration. Secondly, a purification step using a Diaion HP-20 macroporous resin column effectively concentrated the target active compounds in the final extract. The application of these novel extraction and purification methods significantly improves the quality of herbal preparations.
Keywords: Reflux-assisted extraction, Microwave-assisted extraction, Response surface methodology, Macroporous resins, Green chemistry
Type of Study: Research |
Subject:
Pharmacognosy & Pharmaceutics
Received: 2025/04/16 | Accepted: 2026/04/26 | Published: 2026/05/6
Received: 2025/04/16 | Accepted: 2026/04/26 | Published: 2026/05/6
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