en فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <div style="text-align: justify;"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#222222">Background:</span></span></span></b> <span style="font-size:10.0pt"><span style="background:white"><span new="" roman="" style="font-family:" times=""><span style="color:black">The flavonoid-rich roots of <i>Scutellaria litwinowii</i> have therapeutic potential, but traditional extraction methods are inefficient. This study developed an optimized protocol using modern extraction techniques to maximize extract yield. <b>Objective:</b> The roots of <i>S. litwinowii</i> contain valuable flavonoid compounds and exhibit biological properties. Response surface methodology and macroporous resins were employed to increase the extraction yield. <b>Methods:</b> Extraction of the plant roots was conducted according to a design optimized with Design-Expert&reg; software. A comparative analysis was performed between reflux-assisted extraction (RAE), microwave-assisted extraction (MAE), and traditional maceration. Subsequently, phenolic and flavonoid compounds were enriched using a Diaion HP-20 resin column. <b>Results:</b> MAE under optimized parameters yielded a higher total phenolic content compared to conventional 72-hour maceration. Moreover, both MAE and RAE demonstrated significantly higher total flavonoid yields than the maceration method. Furthermore, the Diaion HP-20 resin column increased the amount of active compounds in the <i>S. litwinowii</i> extract by approximately 1.75 times. <b>Conclusion:</b> This study employed two principal strategies for the enrichment of phenolic and flavonoid compounds. Firstly, RAE and MAE demonstrated significantly higher efficiency compared to traditional maceration. Secondly, a purification step using a Diaion HP-20 macroporous resin column effectively concentrated the target active compounds in the final extract. The application of these novel extraction and purification methods significantly improves the quality of herbal preparations.</span></span></span></span></div> Reflux-assisted extraction, Microwave-assisted extraction, Response surface methodology, Macroporous resins, Green chemistry 69 83 http://jmp.ir/browse.php?a_code=A-10-7179-1&slc_lang=en&sid=1 Mansoureh Tavakkoli-Bardaskan tavakkolib3@mums.ac.ir 100319475328460049695 0009-0006-5105-4609 No Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran Javad Asili AsiliJ@mums.ac.ir 100319475328460049696 100319475328460049696 No Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran Samad Nejad Ebrahimi s_ebrahimi@sbu.ac.ir 100319475328460049697 100319475328460049697 No Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, IranOrcid: 0000-0003-2167-8032 Motahareh Boozari bouzarim@mums.ac.ir 100319475328460049698 100319475328460049698 Yes Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran