Background: The fig tree latex has been identified as a rich resource of "ficin" an enzyme protein. This enzyme has a vast application in food substances, pharmaceutical and clinical diagnosis, Fig plant can be harvested well in Khorasan province. Objective: To determine optimum organ and season to retrieve latex for different purposes. Method: In this study the measuring of the enzyme activity was based on its effect of casein substrate. Enzymatic activity was compared with standard papain solution activity. In this research calcium ion concentration as an enzyme activator was studied. Findings: The results show that the activity of the Khorasan native fig latex was 50-100 fold more than papain. The amount and the activity of ficin have been variant in different organs of the tree, and also varied with season. Calcium ion concentrations are also variable in different organs and are also season dependent. There is a relationship between calcium ion concentrations and specific activity of ficin. Results: The optimum season for latex collection is fall and the best organ is the branch ends.

Background: Scrophularia oblongifolia belongs to the Scrophulariaceae family, commonly known as the water figwort in Iran. It has been known as a traditional medicinal plant in Iran. Objective: This study was designed to investigate the phytochemicals and antimicrobial activity of ethyl acetate, methanol, MeOH: H₂O (50:50 v/v), and n-hexane extract of Scrophularia oblongifolia aerial parts, growing in the Kangavar region in the Kermanshah province of Iran. Methods: Compounds of ethyl acetate extract were isolated by chromatographic techniques (HPLC-PDA-MS/MS-Q-TOF) and their structures were elucidated by spectral methods (1D and 2D NMR). The antifungal and antibacterial activities of some new compounds and ethyl acetate, methanol, and n-hexane extract were evaluated. Results: Two new polyphenol iridoid glycosides with phenylpropanoid moiety namely scrophuloside A5 and scrophuloside A6; two known phenylpropanoid glycosides namely scrophuloside A3 and scrophuloside A4, and two known iridoid glycosides namely aucubin and harpagoside were isolated from S. oblongifolia. Pure compounds showed high antifungal activities with MIC values ranging from 0.35 to 0.74 µg/ml against C. albicans and high antibacterial activities with MIC values ranging from 0.70 to 1.48 µg/ml against G. vajinalis. n-Hexan extract showed moderate antibacterial and antifungal activity, while methanol and methanol:H₂O (50:50 v/v) extract showed no antimicrobial activities. Conclusion: Based on these findings, compounds isolated from S. oblongifolia have demonstrated potential antibacterial and antifungal activities.