Showing 19 results for Mehdipoor
H Hajimehdipoor , Sh Rezazadeh , A Hadjiakhoondi , M Pirali Hamedani , A Rezaee , H Zahedi , N Kalantari Khandani ,
year 6, Issue 23 (9-2007)
Abstract
Background: Passiflora has several species that two of them named P. incarnata and P. caerulea are cultivated in Iran. Many investigations have been carried out on therapeutic effects of the genus but only P. incarnata is approved for use in pharmaceutical dosage forms for anxiety and insomnia. Objective: The purpose of this study is differential determination of P. incarnata and P. caerulea in medicinal plants and related herbal drugs. Method: In this investigation, microscopic properties, total ash, acid-insoluble ash and total flavonoids calculated as hyperoside of P. incarnata and P. caerulea were determined. In addition, TLC chromatogram regarding flavonoids and HPLC chromatograms of P. incarnata and P. caerulea ethanol extract, Passipay tablet and drop (Iran Darouk Co.), Passiflora drop and dry extract (Vogel Co.) and Passiflora drop (Curarina Co.) were also compared. Results: No significant difference between total ash, acid-insoluble ash and total flavonoids in two species were found. But they were very different in microscopic properties. TLC and HPLC chromatograms are very different as well. Conclusion: The aerial parts of the two plant species can be well distinguished by microscopic method. The plant extract either itself or in pharmaceutical dosage forms, can be determined by HPLC or TLC methods. Since sample preparation is very simple and doesn’t require complex procedures, the above-mentioned methods seem to be efficient.
H Hajimehdipoor , H Zahedi , N Kalantari Khandani, Z Abedi , M Pirali - Hamedani, N Adib ,
year 7, Issue 26 (5-2008)
Abstract
Background: Artemisia absinthium L. (common wormwood) has been widely used in traditional medicine. The plant contains toxic components: α and β-thujone, therefore, it should be used with caution. Objective: Since common wormwood is considered to be a toxic plant, we decided to evaluate toxic compounds quantities in those used in Iranian folk market. Method: Five samples were purchased from Tehran markets. Essential oil of the flowers was obtained by water distillation. Essential oils were injected to gas chromatograph and their thujone content were determined according to the calibration curve. Results: The results showed that α-thujone is absent in all samples and β-thujone content in the essential oils is much less than its amount reported from other countries. Conclusion: Since the quantity of toxic compounds are low in the plant growing in Iran, it is proposed that Artemisia absinthium L. can be studied for its some medical uses.
H Hajimehdipoor, Y Amanzadeh, T Hasanloo, M Shekarchi , Z Abedi , M Pirali Hamedani ,
year 7, Issue 27 (8-2008)
Abstract
Background: Glycyrrhiza glabra L. has been used in respiratory diseases, gastric and duodenal ulcers and liver insufficiencies for many years. The plant grows in different regions of Iran. Since our country is considered one of the greatest exporting countries of the plant roots, investigation of licorice quality from different parts of Iran and determination of the best area for its growing seems to be important. Objective: According to validated references, glycyrrhizic acid percentage and water-soluble extractives are determinative of quality therefore, in this investigation, these two factors have been measured in the plants collected from different regions of Iran. Method: Roots of the plant were collected from Kerman, Kermanshah, Sirjan, Sarhadd (Fars province), Istahban (Fars province), Ghasredasht (Fars province), Najaf Abad (Isfahan province), Ganjnameh (Hamedan province), Ekbatan (Hamedan province), Mahabad, Khorram Abad and Ardabil and water-soluble extractives using maceration method and glycyrrhizic acid percentage with HPLC technique were determined in each sample. Results: The results showed that the amount of water-soluble extractives and glycyrrhizic acid are high in samples from Kermanshah, Sarhadd and Kerman while these two factors are the lowest in the plant roots collected from Ekbatan and Ganjnameh. Conclusion: The plant roots collected from Kermanshah, Sarhadd and Kerman have the highest quality and are the best for medicinal purposes, sweetening and flavoring agent and for exporting as well.
H Hajimehdipoor, M Khanavi , H Zahedi , Z Abedi , N Kalantari Khandani , N Adib , M Pirali Hamedani ,
year 8, Issue 31 (9-2009)
Abstract
Background: Thymus is a widely used medicinal plant in food and pharmaceutical industries. Among different species of Thymus, T. vulgaris is used more than other species in therapeutic dosage forms. Objective: This study was conducted to differentiate various Thymus species by TLC fingerprint. Methods: In order to identify and differentiate various species of Thymus, TLC fingerprints of essential oil, dichloromethane, ethylacetate and n-butanol extracts of four available species named T. vulgaris, T. pubescens, T. citriodorus and T. daenensis were obtained and compared with each other. Results: The results showed that the TLC chromatograms of essential oil and n-butanol extract can not be used as differential identification for the above-mentioned species while the ethylacetate and dichloromethane extracts are more reliable to be used for TLC fingerprints. TLC chromatogram of ethylacetate extract is specific for identification of T. pubescens and T. vulgaris while T. citriodorus and T. daenensis can be best identified by their TLC fingerprints obtained from dichloromethane extract. Conclusion: For best differentiation of various Thymus species, different extracts of the plants should be used.
H Hajimehdipoor , M Khanavi , M Shekarchi , Z Abedi, M Pirali Hamedani ,
year 8, Issue 32 (12-2009)
Abstract
Background: Echinacea spp. have been used in traditional medicine for many years. Recently, many investigations have been done on this plant and they showed that the plant is an immunostimulant agent. The plant contains different components such as flavonoids, phenolic compounds and isobutylamides. Phenolics are one of the most important agents in immunostimulant effects of the plant therefore, determination of a method for the best extraction of these compounds from the plant is important. Objective: In this research, the effects of solvent, extraction procedure, plant particle size and plant:solvent ratio on extraction of phenolic compounds of Echinacea purpurea (L.) Moench have been investigated. Methods: Different polar and non-polar solvents from hexane to acidic water were used in order to determine the best solvent for phenolics extraction. Moreover, the methods of maceration, sonification, perculation, digestion and continuous extraction (by use of soxhelet apparatus), different particle sizes of the plant powder and various plant:solvent ratios were examined as well. Results: The results showed that the best method for extraction of phenolics is methanol:water 80:20 as solvent, method of digestion (2h, 50 °C), particle size of 300 µm for the plant powder and plant: solvent ratio of 1:200. Conclusion: It can be concluded that in order to efficient extraction of phenolic compounds from Echinacea purpurea aerial parts, special condition should be considered.
H Hajimehdipoor , Se Sadat-Ebrahimi , Y Amanzadeh, M Izaddoost , E Givi ,
year 9, Issue 33 (supplement 6 2010)
Abstract
Background: The seeds of Trigonella foenum-graecum L. (Fenugreek) are used in Iranian traditional medicine as blood sugar lowering agent. According to last investigations, hypoglycemic property is related to the amino acids of the plant specially 4-hydroxyisoleucine.
Objective: In this research, identification and quantitative determination of 4-hydroxyisoleucine in Fenugreek seeds from Shahreza, central part of Iran, has been investigated.
Methods: Powdered seeds of the plant were defatted and then extracted by using ethanol 50%. In order to isolation of 4-hydroxyisoleucine from the extract, ion exchange chromatography was used and the amino acid content was determined by using high performance liquid chromatography technique.
Results: The results showed the presence of 0.4% 4-hydroxyisoleucine in the plant seeds which is considerable amount of the amino acid in the plant collected from Iran.
Conclusion: According to the effects of 4-hydroxyisoleucine such as antihyperglycemic and antihyperlipidemic properties, fenugreek can be considered as an herbal remedy in supportive treatment of these disorders.
H Hajimehdipoor , N Samadi , V Mozaffarian , N Rahimifard , Sh Shoeibi , M Pirali Hamedani ,
year 9, Issue 33 (supplement 6 2010)
Abstract
Background: Oliveria decumbens Vent. is an endemic plant of Flora Iranica that grows in high temperature areas of south and west of Iran. It is used for indigestion, diarrhea, abdominal pain and fever in traditional medicine.
Objective: In this investigation, chemical composition and antimicrobial effects of Oliveria decumbens has been studied.
Methods: The essential oil of aerial parts of Oliveria decumbens was obtained by hydrodistillation and analyzed by GC-MS. The antimicrobial activity of the essential oil was also investigated against three Gram positive bacteria, two Gram negative bacteria and two fungi using disc diffusion method.
Results: 10 components were identified in the essential oil of Oliveria decumbens. The main components were γ-terpinene, myristicin, thymol, ρ-cymene and carvacrol. The essential oil showed high antimicrobial activity against Gram-positive bacteria, Escherichia coli, Aspergilus niger and Candida albicans but low antibacterial effect against Pseudomonas aeroginosa.
Conclusion: High amount of thymol, carvacrol and ρ-cymene in Oliveria decumbens essential oil may be a reason of antimicrobial effects of the plant and the plant essential oil can be considered as a natural source of preservatives in food and cosmetic industries.
N Rahimifard, H Hajimehdipoor, Mh Hedayati, O Bagheri, H Pishehvar, Y Ajani,
year 9, Issue 35 (9-2010)
Abstract
Background: Mentha species are widely used in traditional medicine mostly as anti-flatulence. Nowadays, their usage as flavor and preservative in food, cosmetic and pharmaceutical industries has been developed. Moreover, cytotoxic effects of some Mentha species have been reported. Objective: In this study, cytotoxic properties of Mentha piperita, M. spicata, M. aquatica, M. crispa, M. pulegium and M. longifolia have been investigated. Methods: Different concentrations of essential oils and total extracts of six Mentha species were tested by MTT assay against Vero, Hep2 and Hela cell lines. Results: The results showed that all samples were toxic against Vero, Hela and Hep2 cell lines (IC50 28.1-166.2 µg/ml). Conclusion: All examined Mentha species extracts and essential oils have cytotoxic effects but some of them could be considered as potent toxic agents.
N Rahimifard, H Hajimehdipoor, B Pirouz , F Bagheri , O Bagheri , Ks Mirdamadi ,
year 9, Issue 35 (9-2010)
Abstract
Background: Essential oils from Elettaria cordamomum (cardamom oil) and Lavandula angustifolia (Lavender oil) are used a lot in food and Drug Industry. Cardamom oil traditionally used as spice in food now is increasingly used as diuretic, sedative and for gastrointestinal disease. Lavender oil, traditionally used as an antiseptic agent, is now widely used as a relaxant, carminative, and sedative in aromatherapy. Objective: They both are used as flavouring additives in food and medical industry. It’s very important to know their mutagenic potential. Meanwhile, the growth of cancer disease and insufficient chemical treatments are among main reasons for the antimutagenic effect of essential oils to be assessed. Methods: In this study we investigated the mutagenic and antimutagenic activities of cardamom oil and lavender oil by the bacterial reverse mutation assay in salmonella typhimurium TA98 and TA100 strains with and without S9 (microsomal mutagenesis assay) for 7 dilutions of each essential oils. Results: The mutagenicity effects were not seen in all dilutions of each essential oils, and antimutagenicity effect was seen in 0.40 and more concentration (mg/plate) of Elettaria cordamomum by the bacterial reverse mutation assay in salmonella typhimurium TA98 strains without S9. Conclusion: Assessment of genotoxic potential and identification of mutagenic components of essential oils has been considered widely after their increasing consumption rate, in order to investigate possible new activities of herbal essential oils like antimutagnic effect possibly leading to new and safer products. Although the antimutagenic activity of lavender oil is an interesting finding, further studies are required to identify the components responsible for its antimutagenic action.
Sh Shoeibi , H Hajimehdipoor, N Rahimifard , Sh Rezazadeh , T Hasanloo , F Bagheri, A Amini ,
year 9, Issue 36 (12-2010)
Abstract
Background: Helicobacter pylori infection is common in gastrointestinal tract and lead to gastritis, peptic ulcer and finally stomach cancer. Many synthetic drugs are used to eradicate the bacteria but there are some problems in treatment due to drug resistance and frequent relapses of infection. Herbal drugs are very important in the case. Glycyrrhiza glabra is used for treatment of H. pylori infection. This plant grows in different regions of Iran, therefore, investigation of anti H. pylori effects of the plant collected from different growing area is important. Objective: The aim of this study is determination of MIC of licorice roots collected from different regions of Iran against H. pylori in order to introduce the best growing area of the plant. Methods: Roots of the plant were collected from Kerman, Kermanshah, Sirjan, Sarhadd (Fars province), Istahban (Fars province), Ghasredasht (Fars province), Najaf Abad (Isfahan province), Ganjnameh (Hamedan province), Ekbatan (Hamedan province), Mahabad, Khorram Abad and Ardabil and extracted by using ethanol 80%. MIC of each extract was determined against three clinical isolated strains of H. pylori which obtained during endoscopy procedure. Results: The results showed that susceptibilities of three clinical isolated bacteria to the licorice extracts are different. Among the studied extracts, licorice roots collected from Ardabil showed the lowest MIC (at least: 125 ppm) and all strains were almost resistant to the extract from Mahabad. Conclusion: It is concluded that drug resistance is observed in herbals just like synthetic drugs. Moreover, in order to achieve best therapeutic results, before using an herbal preparation for treatment a disorder, growing area of a medicinal herb should be considered.
Ar Gohari , H Hajimehdipoor , S Saeidnia , Y Ajani , A Hadjiakhoondi ,
year 10, Issue 37 (3-2011)
Abstract
Background: Medicinal plants (especially belong to Lamiaceae family) are potential sources of new drugs to improve the treatment of diseases whose treatment is associated to anti-oxidative agents. In this paper, the Ferric Reducing Ability of Plasma (FRAP) activity of some Lamiaceae and Apiaceae species, has been evaluated. Methods: Aerial parts of the plants were dried, cut into small pieces and extracted with ethyl acetate and methanol, respectively, by percolation at room temperature. Different concentrations of the plant extracts were investigated for antioxidant power using FRAP assay. Results: The extracts showed a considerable antioxidant effect from 16.36 mmol of FeSO4/100 g dry plant equivalents in Scutellaria tornefortii (AcOEt extract) to 404.12 mmol of FeSO4/100 g dried plant in Salvia macrosiphon (MeOH extract). Conclusions: All the plant samples possessed antioxidant activity, while Salvia macrosiphon (MeOH extract), Pimpinella tragioides (MeOH extract) and Salvia limbata (AcOEt extract) showed significantly the highest results and Scutellaria tornefortii (AcOEt extract) showed the lowest power. Antioxidant activity of these species might be due to the presence of flavonoids, rosmarinic acid, coumarins even monoterpenes (like myrcene) in the plant extracts.
M Sepand , M Soodi , M Soleimani , H Hajimehdipoor ,
year 11, Issue 42 (5-2012)
Abstract
Background: The accumulation of beta-amyloid peptide (Aβ) in the brain, is the main characteristic of Alzheimer's disease (AD) that cause oxidative stress and neurotoxicity. Melissa officinalis can protect cells against oxidative damages. This plant is also used in traditional medicine as a memory enhancer and diseases that are associated with neurological disorders. Objective: This study investigated the effects of ethanolic extract of Melissa officinalis on Aβ induced neurotoxicity and its antioxidant mechanism. Methods: Aerial parts of Melissa officinalis were extracted with ethanol 80% using maceration method. PC12 cells were incubated with the extract prior to incubation with Aβ and cell toxicity, production of reactive oxygen species (ROS), lipid peroxidation and glutathione peroxidase activity were measured 24 h later. Results: Incubation of PC12 cells with Aβ significantly caused cell death in PC 12 cells, this was accompanied by increasing in ROS and lipid peroxidation but decreasing in glutathione peroxidase activity. Pretreatment with Melissa officinalis extract significantly protected PC 12 cells against Aβ induced toxicity and attenuated Aβ induced changes in oxidative stress biomarkers in PC 12 cells. Conclusion: Melissa officinalis extract, prevents Aβ induced neurotoxicity through attenuating oxidative stress. It may act as an ROS scavenger and can be a candidate for AD therapy.
M Shekarchi , H Hajimehdipoor , F Naghibi, L Ara , H Moazzeni Zehan ,
year 12, Issue 46 (5-2013)
Abstract
Background: Nowadays, one of the methods for treatment of Alzheimer is using of acetylcholinesterase inhibitors (AChEIs). Discovering the drugs with better effects and fewer side effects especially from natural sources is the aim of many researchers. Objective: In the present investigation, AChEI effects of six Ferula species have been studied. Methods: Total extracts of six species of Ferula including F. persica var. persica, F. hezarlalezarica, F. ovina, F. oopoda, F. hirtella and F. szowitsiana were obtained by using methanol 80% and different fractions of the species were prepared by using hexane, chloroform, ethyl acetate, methanol, methanol 50% and water, respectively with maceration method. AChEI activity of each extract in concentration of 300 µg/ml was determined by Ellman method in 96 -wells plates in 405 nm. Results: The results revealed that among examined samples, only F. persica var. persica chloroform fraction showed reasonable AChEI activity (27.3%) while other extracts had trace activity or no effect. Conclusion: It seems that relatively non-polar components of F. persica var. persica had AChEI activity. Since, sesquiterpene coumarins are the major compounds in Ferula species, they may be considered as AChEI agents in Ferula persica var. persica, but more investigations are necessary to establish the idea.
Mahnaz Khanavi, Tahereh Hasanloo, Homa Hajimehdipoor,
year 18, Issue 69 (3-2019)
Abstract
Background: Melia indica and M. azedarach (Meliaceae) are widely used in traditional medicine. These species are considered as natural pesticides. Their major compound is azadirachtin (AZD) which is considered as the marker for quality control of these species.
Objective: In the present investigation, AZD content of the leaves and fruits (comprising the whole fruit, peel, pulp and seeds) of M. indica and M. azedarach have been determined.
Methods: The leaves and fruits of M. indica and M. azedarach were collected from Bandar Abbas and Gorgan, respectively and identified. The peel, pulp and seeds were separated from each other. One gram of each sample was extracted using methanol. AZD content of each extract was determined by HPLC with following condition: C18 column, mobile phase: acetonitrile: H2O (60:40), flow rate: 1 mL/min, wave length: 227 nm and run time: 6 min.
Results: The results revealed that the fruits of the species contained higher amounts of AZD compared to the leaves. Comparing the species, AZD content of M. indica fruits was about two folds in comparison with M. azedarach fruits; also, the content of AZD in the seeds of M. indica was more than peel and pulp of the plant fruits. AZD content was high in peel of M. azedarach and was trace in pulp and seeds of the plant fruits.
Conclusion: It is concluded that M. azedarach peel could be suggested to be used regarding pesticide properties while it is better to use the whole fruits of M. indica for insecticidal effects.
Mahnaz Khanavi, Leila Ara, Narjes Khavasi, Homa Hajimehdipoor,
year 19, Issue 73 (3-2020)
Abstract
Background: Capparis spinosa is used in different countries for prevention and treatment of various diseases. Its fruits are usually used in processed form especially with vinegar. Objective: The aim of the present study was investigating the effect of processing on some phytochemical constituents and biological activities of C. spinosa. Methods: The fruits were processed with grape vinegar. Total phenolics and alkaloids contents of the raw and processed fruits were determined by Folin-Ciocalteu and titration methods, respectively. For quantitation of rutin, quercetin and α-tocopherol, R-HPLC was used. Cytotoxic activity of the fruits was determined by MTT assay. Antioxidant properties were evaluated by DPPH and FRAP techniques. Fingerprinting of raw and processed fruits were prepared using TLC and evaluated by TLC densitometry instrument. Results: The results demonstrated that while total phenolics, total alkaloids and quercetin contents were decreased in the processed fruits, rutin concentration remained unchanged. Antioxidant activities of processed fruits increased using both DPPH and FRAP methods. Raw and processed fruits showed no cytotoxic effect on MCF-7, HepG-2 and MDBK cell lines up to the concentration of 100 µg/ml. The fingerprints of the fruits were different which admitted the change in the fruit constituents due to processing. Conclusion: It seems that processing with vinegar lessens the unpleasant taste of the plant due to alkaloids and increased the antioxidant effects; therefore, it would be more suitable for use in some diseases such as diabetes and hepatitis as it is used in folklore and traditional medicine.
Somayeh Esmaeili, Saleh Omid-Malayeri, Homa Hajimehdipoor, Hamid Reza Rasekh, Hamid Reza Moghimi, Soheil Omid-Malayeri, Roya Yaraee, Mohammad Reza Jalali Nadoushan,
year 19, Issue 76 (11-2020)
Abstract
Background: Turmeric (Curcuma longa L.) is widely used in traditional medicine. In Iranian folk medicine, turmeric and egg yolk mixture is used for inflammation due to dislocations and strains. Since lecithin (one of the component of egg yolk) is an absorption enhancer, it seems this phospholipid can increase turmeric absorption and its anti-inflammatory effect. Objective: In order to find the role of lecithin in the folklore formulation, anti-inflammatory effects of ointments containing turmeric extract and lecithin have been studied. Methods: Ointment base was prepared using bees wax, vaseline, liquid paraffin and eucerin. Then, hydroalcoholic turmeric extract (2.5 % and 5 %) with lecithin (5 % and 15 %) or without lecithin were added to the ointment base. Anti-inflammatory effect of the ointments was assessed in arthritis model in rat using complete Freund’s adjuvant. Ointments were used daily on the inflamed joints for 20 days. Finally, arthritis index, TNF-α concentration and histopathological changes of joints were determined. Results: The results showed that arthritis index has decreased in all groups but it was significant in groups of turmeric 2.5 % and turmeric 5 % with lecithin 15 %. TNF-α was decreased in all samples but reduction was more significant in group turmeric 2.5 %. Histopathological changes were significantly less in turmeric groups compared to ointment base group. Conclusion: It seems that local healers use egg yolk as a binder in formulation. In other words, turmeric 2.5 % ointment is preferable compared to other formulations.
Rasool Choopani, Homa Hajimehdipoor, Karam Molaei, Haniye Kashafroodi, Fatemeh Tavakolifar, Leila Ara,
year 20, Issue 78 (5-2021)
Abstract
Background: Coriander Triphala is one of the famous drugs in traditional medicine which is consisted of Terminalia chebula, T. bellirica, Phyllanthus emblica, Coriandrum sativum, almond oil and honey. Traditional dosage forms should be converted to modern forms for better acceptance and suitable characteristics and stability. Objective: In the present investigation, the traditional form of Coriander Triphala was converted to film coated tablet and quality control of the tablet was performed. Methods: The fruits ofT. chebula, T. bellirica, Ph. emblica, C. sativum in equal proportions along with almond oil and honey in different proportions were used for tablet formulation with other excipients. Sixteen formulations were made and after pre-formulation studies, twelve of them were selected for making tablet. Prepared tablets went through primary quality control tests such as weight variation, friability, hardness and disintegration time. Finally, the best formulation was coated by green colored water soluble material and its physicochemical characteristics were determined. Results: Among different formulations, the tablet consisted of 98 mg of each species, 14 mg almond oil, 148 mg honey along with lactose, Avicel PH-102, croscarmellose sodium, PVP K30, magnesium stearate and silicone dioxide was the best one. Weight variation, hardness, disintegration time, total tannins content as pyrogallol were found 1192 mg ± 5 %, 20 kp, 25 min and 64.19 mg/tablet, respectively. Over 90 % of tannins were released after 60 min during dissolution test. Conclusion: The formulated tablet with suitable characteristics is a good substitution for traditional form and could be produced in industrial scale after complementary clinical trial studies.
Somayeh Esmaeili, Behnaz Keramatian, Haniyeh Kashafroodi, Rasool Choopani, Homa Hajimehdipoor,
year 21, Issue 82 (5-2022)
Abstract
Background: Capparis spinosa is used in Persian medicine for treatment of various diseases. In order to easier use, better patient’s acceptance and more stability, preparing a suitable dosage form is necessary. Objective: The aim of the study was preparing tablet form from caper fruits and quality assessment of the product. Methods: The fruit extraction was performed using ethanol 80% and maceration technique and the extract was dried by freeze dryer. Eight formulations were made using lactose, avicel PH-102, SiO2 and magnesium stearate. The best formulation was failed during stability tests; therefore, the extract was dried by spray drying method along with maltodextrin and SiO2 as excipients. Eight formulations were prepared using lactose, avicel PH-102, croscarmellose sodium, PVP K30 and magnesium stearate and the best one was selected. Physicochemical and microbial assessments were performed on the selected formulation and stability tests were done in 40°C and 75% humidity as well as 30°C. Results: Caper tablets with freeze dried extract were deformed and their color changed but tablets with spray dried extract were stable in 30°C. They were oblong, green-blue, biconvex, scored tablets with 20.3×9.9×6.7 mm dimensions. Weight, hardness, disintegration time, rutin assay and dissolution were 1115 mg ± 10%, 18.33 ± 1.52 kp, 15±3.5 min, 0.58 ± 0.02 mg/tab and 93.03 ± 3.61 % in 60 min, respectively. Conclusion: Caper tablets are good candidate for production in industrial scale after in vivo and clinical studies. Moreover stability assessment of the tablets should be performed in suitable packaging in long term study.
Behnam Roozbeh, Mohammad Mehdipoor, Gholam Rasul Mohammad Rahimi, Mehrdad Fathei, Tom Clifford, Bibi Pourmirza,
year 23, Issue 90 (5-2024)
Abstract
Background: The use of dietary herbal products as potential anti-obesity agents has gained considerable traction in recent years. Objective: The purpose of the current study was to assess the effects of silymarin supplementation with and without exercise training on a high-fat diet-induced metabolic disorder in male Wistar rats. Methods: 36 male Wistar rats were divided into six groups; 1) control group with a regular diet; 2) high-fat diet (HFD); 3) HFD + Silymarin 25 (S25); 4) HFD + Silymarin 50 (S50); 5) HFD +S25 + ART (aerobic resistance training); 6) HFD + S50+ART (n = 6 for each group). HFD + S25 + ART and HFD + S50 + ART groups performed aerobic exercise three days/week and resistance exercise two days/week. Blood specimens were obtained for biochemical assessments and gene expression at the end of the 12-week intervention. The data were analyzed using one-way ANOVAs and Tukey’s post hoc test with SPSS21 at a significance level of P < 0.05. Results: Final body weight, the levels of glucose, insulin, and Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) in all of the treatment groups were significantly less compared with the HFD group (P < 0.05). Lipid profile was significantly improved in treatment groups in comparison with the HFD group (P < 0.05). Leptin and Neuropeptide Y (NPY) levels were significantly lower in HFD + S50, HFD + S25 + ART, and HFD + S50 + ART groups compared to HFD group (P < 0.05). Exercise plus silymarin consumption (HFD + S25 + ART and HFD + S50 + ART) increased Peptide YY levels (PYY) (P= 0.013 and P < 0.001, respectively). Conclusion: Our findings suggest that combined silymarin consumption and exercise training is a promising non-pharmacological treatment for multiple simultaneous HFD-induced risk factors.
