Showing 13 results for Amiri
A Esmaili , H Amiri ,
year 5, Issue 20 (12-2006)
Abstract
Background: Smyrnium cordifolium belongs to Umbelliferae family which growing in the west and southwest of Iran and has nutritive and medicine use in the regions. Aim: Identification of chemical compositions of essential oil of S. cordifolium grown in different regions of Lorestan province and study effects of environmental factors on it. Methods: This plant was collected from five differen regions of Lorestan province and was investigated for quatitative and qualitative changes of its essential oil. The oils was injected to GC for identification of constituents. Results: Yields of essential oil were 0.07% to 0.55% in these different regions. The most amount of essential oil was related to Poldokhtar and the least amount to Aligoodarz. In the essential oil of different regions two oxygenated sesquiterpen Curzerene and Curzerenone had the most amount. Among common and remarkable components we can mention to Germacrene-D, Isopimarol and Phyllocladanol. In Kouhdasht and Khoramabad, Curzerene was the main component with 39.4 and 34.2 percentages respectively, and in Poldokhtar, Nourabad and Aligodarz it was Curzerenone with 49.4, 33.8 and 35.6 percentages.Also, in different regions Germacrene-D is abundant in the oil Discussion: The identified components of essential oil of different regions showed the differences and similarities from quantitative and qualitative point of view. GC/MS analysis revealed that the sesquiterpen compounds were the main components in different regions. α-pinene and β-pinene were just observed in Norabad and Aligodarz.
H Amiri ,
year 6, Issue 21 (Supplement 3 Anti-microbial plants 2007)
Abstract
Background: Allium jesdianum Boiss. belongs to Liliaceae family, which grows widly in west and northwest of Iran and has nutritive and medicine uses in the regions.
Objective: Identification of chemical composition and antibacterial activity of the essential oil and different extracts of Allium jesdianum.
Methods: The aerial parts were collected in flowering stage. The essentail oil from aerial parts, which was obtained by Hydrodistillation, was subjected to GC and GC/MS. The antibacterial effects of essential oil and aqueous, methanolic and ethanolic extracts of Allium jesdianum were studied by digging hole and measuring diameter of grows inhibiting ring and Gentamicine antibiotic was used for comparison.
Results: The yield of essential oil obtained by hydrodistillation from dried plant material was 0.1% w/w. The major components were found to be: Trisulfide dimethyl (22.34%), Hexadecanoic acid (19.03%), Phytol (12.82%), Disulfide methyl-1-(methylthio) ethyl (9.25%), Pentacosane (8.03%), Curzerene (7.62%). The study of antibacterial activity showed that the ethanolic extracts have stronger antibacterial effects compared with essential oil and other extracts.
Discussion: In conclusion, the data reported in our study showed that the sulfide compounds represented the most abundant compounds as the other Allium species. The antibacterial activity of oil and extracts of A. jesdianum can be attributed of sulfide and terpenoides compounds.
H Amiri ,
year 6, Issue 21 (3-2007)
Abstract
Background: The Prangos genus which belongs to Umbelliferae family is represented 15 species in Iran. Jashir (Prangos ferulacea Lindl.) is long and permanent herb which is mainly used as rich herb in animal feeding. Moerover the essential oils were mainly used in nutrative and pharmaceutical industries and antibacterial agents. Objective: Identification of chemical composition of essential oil of Prangos ferulacea grown in Lorestan province and its comparison with other regions, and to study antibacterial activity. Methods: This plant was collected from Broujerd mountains of Lorestan province in Iran. Voucher specimens were deposited at the herbarium of Natural Resource and Agriculture Center of Lorestan Province (NO: 5783). The air-dried aerial parts were subjected to hydrodistillation method. Identification of essential oil costitiuents was made by GC and GC/MS. After drilling wells on medium oils dissolved in hexane and 40μL from solutions in each well was poured. Results: The yield of essential oils obtained by hydrodistillation from dried plant material was 1.6% w/w. The main constituent of this oil were α-pinene (36.6%) and β-pinene (31.1%). The results of the antibacterial activity showed that the oil was active against of most of the positive and negative bacteria specially Staphyllococcus aureus. Conclusion: A large prcentage of the oil was composed of hydrocarbone monoterpens and only identified sesquiterpen compound is β-caryophyllene (3.1 %). Sefidkon and Kuznetsova investigations on analysis of the essential oil of aerial parts and fruits from Jashir demonesterated similarities and differenes with our study. Antibacterial activity of the oil was probably attributed to large content of monoterpene especially α-pinene.
H Amiri ,
year 7, Issue 28 (11-2008)
Abstract
Background: The Teucrium genus belongs to Lamiaceae family and has 13 species in Iran. On the other hand the essential oils which are the plant’s secondary metabolite products are widely used in food, pharmaceutical and cosmetic industry, as antibacterial and antioxidant products. Objective: Identification of the components of essential oil of Teucrium orientale L. subsp. taylori. and its comparison with Teucrium orientale L. subsp. orientale. Methods: This plant was collected from 55 Km. distant of Khoramabad at east of Lorestan province. Voucher specimens were deposited at the herbarium of Natural Resource and Agriculture Center of Lorestan Province (NO: 5783). The air-dried aerial parts were subjected to hydrodistillation method. Identification of essential oil constituents was made by GC and GC/MS. Results: Fourty components were identified in the essential oil and the main constituents were linalool (28.60%), caryophyllene oxide (15.62%), octanol (9.55%), β- pinene (8.75%), β- caryophyllene (7.33%), germacrene-D (4.60%) and 1,8-cineol (4.53%). Conclusion: The comparison of the obtained results from analysis of the oil of Teucrium orientale L. subsp. taylori in this research with other investigations on Teucrium orientale L. subsp. orientale showed that the main components in the oils of two subspecies with a few differences in percentages of the major constituents are similar. But in other components which constituentes lesser percentages of the essential oils, there are some differences. considering the similarities in the components of subspecies of oriental and tylori, perhaps these two subspecies will be placed under the same chemotypes.
A Esmaeili , H Amiri, Sh Rezazadeh,
year 8, Issue 31 (9-2009)
Abstract
Background: The genus Tanacetum (Compositae) is represented by 26 species in the flora of Iran, 12 of them are endemic. One report on the analysis of essential oil of Tanacetum polycephalum Boiss. species has been published. Objective: Aim of this study is to determine the chemical composition of the hydrodistilled oil of Tanacetum pinnatum Boiss. of Iranian origin for the first time. Methods: The essential oil of T. pinnatum obtained by hydrodistillation and analyzed by GC and GC/MS. Results: The major constituents appeared to be camphor (23.2%), α-pinene (8.5%) and camphene (7.7%). Conclusion: In T. pinnatum we identified 25 components representing 98.7%. It was rich in regard to monoterpene hydrocarbons (78.9%). The sesquiterpene fraction was relatively small, representing 19.8%.
R Mohammadi , Mr Shokooh Amiri , Sm Mousavi , A Sepahvand , M Shams Ghahferokhi , Mh Yadegai , Sh Roudbar Mohammadi , Sh Shadzi,
year 9, Issue 36 (12-2010)
Abstract
Background: Aspergillosis is an opportunistic fungal disease caused by different species of Aspergillus. Clinical manifestation and severity of disease related to physiological conditions of host, involved organs and Aspergillus species. Cinnamomum zeylanicum is an evergreen tree 10-15 meter length and belongs to Lauraceae family. It grows in Srilanka. Its essential oil has antimicrobial activity. Objective: Study of Antifungal activity of this essential oil against clinical isolates of Aspergillus and determination of MIC. Methods: Broth microdilution method was used in this research. Essential oil of Cinnamomum zeylanicum was extracted by means of Clevenger apparatus then its MICs on clinical isolates of Aspergillus were calculated by broth microdilution method. Results: Number of samples was twenty seven. MICs are: eight isolates: 1.18 μg/ml, six isolates: 0.59 μg/ml, four isolates: 0.29 μg/ml, five isolates: 0.14 μg/ml and four isolates: 0.07μg/ml Conclusion: In view of the fact that Cinnamomum zeylanicum essential oil was effective against all isolates in this research, we recommend the investigation of its antifungal properties Invivo.
H Amiri ,
year 11, Issue 41 (supplement 8 2012)
Abstract
Background: Salvia multicaulis is a permanent herb belongs to Lamiaceae family which wildely grows in most regions of Iran. This plant is used as flavour and tea.
Objectives: Investigation on the essential oil composition and antioxidant activity of Salvia multicaulis from Lorestan province and comparison of these with other regions.
Methods: The aerial parts of the plant were collected from Broujerd in Lorestan province. The air-dried samples were subjected to hydrodistillation using by Cleavenger apparatus for 2 h (in yield of 0.3%). The essential oil was analyzed by GC and GC/MS. The samples were subjected to screening for their possible antioxidant activity by using DPPH and β-carotene-linoleic acid assay
Results: Forty-two constituents were identified, accounting for 90.96% of total oil. The main components of this oil were bornyl acetate (18.58%), borneol (17.16%), camphor (13.75%), camphene (5.62%) and β-caryophyllene (5.85%).The samples were subjected to a screening for their possible antioxidant activities by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene–linoleic acid assays. In general, in both method the methanolic extract exhibited greater antioxidant activities than essential oil.
Conclusion: Antioxidants act as radical scavengers, inhibit lipid peroxidation and other free adical-mediated processes and are able to protect the human body as well as processed foods from oxidative damage attributed to the reaction of free radicals.
Mm Soltan Dallal , Mh Yazdi, S Aghaamiri , Sh Haghighat Khajavi , Tp Abedi Mohtasab , F Amin Harati, M Gorbanzadeh Meshkani ,
year 13, Issue 52 (12-2014)
Abstract
Background: Staphylococcus aureuse is one of the important pathogens which transmitted by food and has majority of habitant in human and animal community as a pathogen and normal flora. Antibiotic resistant among Staphylococcus aureuse strains is a global health challenge. Objective: Regarding to the different therapeutic and antimicrobial effect of Shirazi Zataria multiflora and Rosemarinus officonalis in present work the antibacterial effect of this extract and its synergistic effect with routine antibiotics was investigated. Methods: In this in vitro study the antimicrobial effect of Shirazi Zataria multiflora and Rosemarinus officonalis extract on methicillin resistant Staphylococcus aureus (MRSA) and other antibiotic resistant strains to tetracycline, erithromycine, trimethoprim, sulfamethoxazol, together with its MIC and MBC were determined. Also synergistic effect of these extracts with these antibiotics was investigated by paper disc method. Results: Shirazi Zataria multiflora, had a significant antibacterial effect against MRSA, and other Staphylococcus aureus resistant strain to tetracycline, erithromycine, trimethoprim, and sulfametoxazol, isolated from food. Conclusion: Production of a suitable herbal medicine with few side effects will give rise to a promising outlook in the treatment of infections caused by antibiotic resistant strains of Staphylococcus aureus.
Hr Amiri , M Mohammadi , S Sadatmand , Taheri E ,
year 15, Issue 58 (4-2016)
Abstract
Background: Ginger Zingiber officinale has been used as a medicine since ancient times.
Objective: This study has been concentrated on identification of chemical compositions of essential oils of Indian and Chinese gingers. Also, antioxidant and cytotoxic activities of Chinese oil were determined.
Methods: The first species was collected from India Province and other was purchased from Iranian markets. The essential oils were obtained by Hydrodistillation in a Clevenger type apparatus and were dried over anhydrous Sodium Sulphat. The yields of essential oils were 1.45 and 1.3% w/w, respectively. Then, the oils stored at +4°C in the dark place until analysis. The chemical compositions of essential oils were analysis by GC and GC/MS method. The identification of the essential oils components was based on calculation of retention Indices and comparison of mass spectra with standards. The Chinese oil was subjected to screening for its possible antioxidant and cytotoxic activities by means DPPH and MTT assays, respectively.
Results: 46 constituents were identified in the essential oils. The major constituents of Indian species were α-Zingiberene (28.25%) β-Sesquiphellandrene (15.65%), α-Curcumene (15.23%) and trans-γ-Cadinene (11.88%). In Chinese species α-Zingiberene (35.67%) β-Sesquiphellandrene (15.27%), trans-γ-Cadinene (9.25%) and E-Citral (6.0%) were main components. In the DPPH test and the MTT test systems, the IC50 values of Iranian ginger essential oil were 80.7 and 72.6 µg/ml, respectively.
Conclusion: The main class of the compounds were found in these species to be sesquiterpenes. Chinese ginger essential oil exhibited the strong cytotoxicity and antioxidant activity at low concentration.
Dr Neda Mohamadi, Peyman Rajaei, Mehran Moradalizadeh, Mohammad Sadegh Amiri,
year 16, Issue 61 (2-2017)
Abstract
Background: The leaves, roots and seeds of Lovage (Levisticum officinale Koch) being used as an herb, vegetable and spice.
Objective: The aim of this study is evaluation of the changes of phytochemical compound in aerial parts of the Lovage plants at different developmental stages as well as antioxidant activity.
Methods: The plants were harvested from Hezar Mountain. After drying, essential oils were extracted by steam distillation. To identify the essential components GC and GC-MS was used. Antioxidant activity of samples was examined by diphenylpicrylhydrazyl (DPPH) assay.
Results: The average yield of essential oil was respectively, 2.3% and 3.1% and 1.5% respectively, in vegetative, Flowering and seed stage. The oil analysis results showed that 21 compounds were identified in the vegetative stage. β-phellandrene (10.7%) and α-Terpinyl acetate (% 38.9) and Curzerene (% 10.6) were the major compound. in the reproductive stage, 22 compounds were identified including β-phellandrene (20.3%) and α-Terpinyl acetate (% 20.4) and γ-Cadinene (12.1%). in the seed stage β-phellandrene (21.1%) and α-Terpinyl acetate (% 25.3) and Sabinene (10.2%) were the highest. Analysis of variance showed that the effect of harvesting time has significant effect on plant height, stem branch number and yield of oil. The Higher antioxidant power was observed respectively in the flowering stage (83%), vegetative stage (68%) and seed stage (60%).
Conclusion: The phytochemical and antioxidant compounds in Levisticum officinale depend on the phenological stage.
Amir Yami, Maryam Hamzeloo-Moghadam, Afshin Karami, Mohyedin Barzegar, Vahid Amiri, Ahmad Gharehbaghian,
year 19, Issue 74 (6-2020)
Abstract
Background: Cancer is a multi-faceted diseases caused cell proliferation in an out of control manner due to accumulation of defects and mutation in their DNA and with an impendence to invade or spread to parts of the body. During last decades new compounds with natural roots have emerged as a new paradigm for effective anti-cancer treatment. Objective: The aim of this study is to evaluate the potential of ergolide a sesquiterpene lactone with multi-functional history to induce apoptosis. Methods: Ergolide from Inula-Oculus-Christi a sesquiterpene lactone from Asteraceae plant was extracted by Traditional Medicine Research Center of Shahid Beheshti University of Medical Sciences. The cytotoxic effects of Ergolide on the acute lymphoblastic leukemia (MOLT4) and PBMC (normal cell line) were investigated at different doses for 48 hours. In this study, MTT assay and acridine orange staining were used. Also, annexin V-PI assays was utilized for further evaluation. In addition the gene expression level of BAX and BCL2 were analyzed by q-RealTime-PCR (quantitive RT-PCR). Results: The results of MTT assay demonstrated the induction of apoptosis and reduction in proliferation of MOLT4 cells treated by ergolide. (P < 0.001). Interestingly Ergolide could be less toxic in normal cells (PBMCs). AO staining and Flow cytometry analysis confirmed a significantly high percentage of autophagic and apoptotic cells compared with control groups respectively (P < 0.05). Conclusion: According to the results, ergolide demonstrated cytotoxic effects on MOLT-4, but further studies are needed to confirm its effectiveness as a complementary agent in the treatment of acute lymphoblastic leukemia.
Saleh Amiri, Reza Fotovat, Bahman Panahi, Alireza Tarinezhad, Seyyed Abolghasem Mohammadi,
year 19, Issue 74 (6-2020)
Abstract
Background: Based on recent statistical survey, the cancer is the third important factor in Iran mortality. Vinblastine and vincristine alkaloids are dominantly biosynthesized in the aerial parts of periwinkle, broadly applied for cancer treatment. Therefore, over production of these alkaloids by using the biotechnological approaches is inevitable. Since the terpenoid indole alkaloids (TIAS) biosynthesis pathway is adjustable, modification of underlying substrate and enzymes concentration by biotic and abiotic elicitors are important approaches for overproduction of these metabolites. Abiotic and biotic are induced the immune systems of periwinkle and subsequently increased the biosynthesis and accumulation of vinblastine and vincristine. Objective : In this study, we reviewed and discussed the impacts of different abiotic and biotic elicitors on TIAS biosynthesis and consequent secondary metabolites over production. Methods: In current study, using related keywords, eligible papers were identified using search motors such as Google and Scopus. Results: Our study indicated that biotic and abiotic elicitors are versatile tools for over-production of valuable metabolites in periwinkle. Conclusion: Based on our knowledge this is the first study on the reviewing of the different elicitors on vinblastine and vincristine and it will be more helpful in the future studies.
Fereshteh Talebpour Amiri, Fatemeh Mirzaee, Seyedeh Nesa Hasanzadeh, Reza Enayatifard, Somayeh Shahani,
year 20, Issue 79 (8-2021)
Abstract
Background: Proper wound management which improves the quality of life and reduces patient costs is required. Lamium album L. has been used in traditional medicine to heal skin wounds and recent studies show anti-inflammatory, haemostatic and antimicrobial properties of this plant. Objective: The effect of the ointment containing methanol extract of L. album aerial parts was evaluated on the full-thickness wound healing in rat model. Methods: Thirty-two Wistar rats (250-300 g) were used to be created a square full-thickness wound on the dorsal cervical area and randomly divided into four groups: I; control, II; ointment base, III; 1 % phenytoin cream and IV; 5 % L. album ointment. Wound size for determination of the percent of wound healing was measured on days 3, 5, 7 and 12 of the experiment. The excisional biopsies were evaluated histopathologically on the 12th day of treatment according to the Abramov score method. Results: The herbal ointment significantly increased fibroblast maturation, collagen deposition and neovascularization compared to the control group. The wound healing rate was significantly increased in the group treated with L. album ointment, same as phenytoin group, on days 3, 7 and 12. Based on the spectrophotometric analysis, the extract contains phenol and flavonoid compounds. Conclusion: It seems that L. album could be considered as a new candidate for further studies in the field of wound healing.
