Showing 7 results for غفاری
E Gharirvand Eskandari , M Doudi , P Shoaei , Sh Ghaffari , M Yaran ,
year 16, Issue 61 (supplement 10 2017)
Abstract
Background: Leishmaniasis, has created enormous global health problem. Side effects, drug resistance and the lack of effective vaccines and to make the new compounds effective due to plant.
Objective: The traditional medical plants such as black alfalfa can be a valuable source of new pharmaceutical agents against leishmaniasis.
Methods: Alcoholic extracts were prepared by maceration method. L. major promastigotes (Leishmania major) in Schneider and then were cultured in RPMI- 1640. Then, using MTT (Methyl Thiazole Tetrazolium), the IC50 (Inhibitory Concentrations 50%) for extract and Glucantime was determined. MTT assay did for each sample, 3 times.
Results: IC50 for alcoholic extract of alfalfa black against L. major promastigotes in vitro after 24, 48 and 72 hours, respectively 165, 98 and 45 micrograms per ml and for Glucantime also equal to 27, 12 and 8 mg l respectively. IC50 between Extract and Glucantime after 24, 48 and 72 hours there was a significant difference (P <0.05). Morphological changes after challenge with meglumine and alcoholic extracts including cell shrinkage, round, dense cytoplasm and the cell was smaller. Presence of alkaloids and flavonoids in alcoholic extracts have been proved.
Conclusion: As regards, plant extract had anti- leishmanial effects in vitro, further works are required to appraise the exact effect on Leishmania agent in animal models.
Saeideh Momtaz, Mahshid Dibaj, Alireza Abdollahi, Gholamreza Amin, Roodabeh Bahramsoltani, Mohammad Abdollahi, Parvin Mahdaviani, Amir Hossein Abdolghaffari,
year 19, Issue 73 (3-2020)
Abstract
Background: Ethnomedicinal plants are of interest for treatment of various wound types. Objective: This study aimed to evaluate burn wound healing potential of the hydro-alcoholic extract of the flowers of Lilium candidum L. in rats. Methods: DPPH free radical scavenging power, total flavonoids, and the phenolic content of the extract were evaluated. Following burn wound induction, 30 Wistar rats were divided into 5 groups; silver sulfadiazine cream 1% (reference standard), eucerin (control), and 5%, 10% and 20% ointments of L. candidum flowers extract (treatment groups). Wound contraction and the percentage of wound healing were measured every day until 14th day and the histological changes were evaluated. Results: DPPH assay of L. candidum extract indicated high antioxidant activity. Total phenolic content of L. candidum extract was equal to 157 mg gallic acid equivalent/g; while total flavonoid was measured as 32.4 mg quercetin equivalent/g dried extract. The mean of wound area was significantly different in three 5%, 10% and 20% extract-treated rats in comparison with the control. Although, 20% ointment of L. candidum flowers extract demonstrated the best wound healing improvement, even better than silver sulfadiazine. Histopathological analysis confirmed the wound healing activity of L. candidum and promoting tissue repair, especially for the ointment containing 20% extract. Conclusion: Regarding the high content of phenolic compounds and flavonoids, besides potent antioxidant properties of the flowers of L. candidum, the use of its ointments led to significant decrease of burn wound area, suggesting that these extract ointments can treat burn wounds and improve the healing processes.
Abdolali Moosavyzadeh, Razieh Jafari Hajati, Farzaneh Ghaffari, Shabnam Khatami, Niki Vakili Zahir, Mohsen Naseri,
year 19, Issue 75 (9-2020)
Abstract
Background: Due to the lack of drug dependence prevention processes and the low effectiveness of treatment for the withdrawal of addiction and undesirable side effects of synthetic drugs, moving towards the use of reverse pharmacology to produce a less costly, effective, and low-risk drug is inevitable. Objective: Considering the long history of traditional medicine in Iran in the field of diagnosis and treatment of diseases, including addiction, this study was done by looking at the original manuscript of traditional Persian Medicine (PM), animal studies and clinical trial of the effects of Daturra stramonium and Hab-o-shefa for treatment of opiate addiction. Methods: This paper includes three sections: the study of PM textbooks, animal studies and clinical trials based on research conducted. Results: In the study of PM texts, two main pharmacologic ways to treat opioid habits have been described, graduation and replacement. In the research done Daturra’s effect on pharmacological withdrawal syndrome in male rats was significantly more than methadone. Hab-o-shefa’s toxicology studies did not show any side effects and tissue damage. Animal studies did not show a significant difference in the total score between the methadone group and Hab-o-shefa group. Clinical studies of Hab-o-shefa on the withdrawal syndrome of opioid dependent patients showed the effect of this product on controlling depression and Signs of deprivation compared to placebo and clonidine. The effect of Hab-o-shefa as a preservative in people with opioid abuse reduced craving, anxiety, and depression over time. Conclusion: The results of our studies showed the effectiveness of hab-o-shefa in all phases of addiction treatment. However, further studies are needed to confirm these findings.
Zahra Tofighi, Mostafa Pirali Hamedani, Saeed Tavakoli, Mir Javad Tabatabaei, Marzieh Rabei, Shamim Mohtadi, Farnoosh Mirghaffari, Maryam Afshani, Farhad Kahrizi, Behruz Khodabandeloo, Saeede Jafari-Nodooshan, Mahdieh Shirzad, Elahe Motevaseli, Saied Goodarzi,
year 19, Issue 76 (11-2020)
Abstract
Background: Apiaceae fruits as common spices used for prevention of many chronic diseases including cancer.
Objective: The present study compared the biological effects of different fruits from various Apiaceae tribes to compare and find the fraction source(s) with potential characteristics for further investigation including cancer prevention.
Methods: Fruits of
Apium graveolens L. (celery),
Bunium persicum (Boiss.) B.Fedtsch. (black cumin),
Petroselinum crispum (Mill.) Fuss (parsley),
Pimpinella anisum L. (anise),
Trachyspermum ammi (L.) Sprague (ajwain),
Coriandrum sativum L. (coriander),
Foeniculum vulgare Mill. (fennel),
Anethum graveolens L. (dill),
Heracleum persicum Desf. ex Fisch., C.A.Mey. & Avé-Lall. (Persian hogweed),
Ferula assa-foetida L. (asafoetida),
Cuminum cyminum L. (cumin) and
Daucus carota L. (carrot) were extracted with 80 % methanol and fractionated by petroleum ether, chloroform, ethyl acetate and methanol, respectively. For different fractions and total extract of all 12 samples, cytotoxicity by brine shrimp test (BST) and MTT assay against cancer and normal cell (foreskin fibroblast cells), antioxidant effects by FRAP, and total phenols by Folin-Ciocalteu method were measured.
Results: The general toxicity of ethyl acetate fractions (mean of data) was higher than others in the brine shrimp test (P < 0.05). The most cytotoxic fractions against colon carcinoma (HT-29), breast adenocarcinoma (MDA-MB-231) and alveolar basal epithelial adenocarcinoma (A549) cell lines were from Ammineae and Peucedaneae tribes while fruits fractions with high phenol contents and antioxidant powers were from Ammineae tribe.
Conclusion: The Apiaceae fruits have significant biological effects, therefore the isolation of phytochemical compounds from active fractions with cytotoxicity is suggested in future studies.
Saeideh Momtaz, Amirhossein Abdolghaffari, Eghbal Jasemi, Bahman Yaghoobvand, Saeed Esmaeilzadeh, Alireza Abdollahi, Mohammad Abdollahi,
year 20, Issue 77 (1-2021)
Abstract
Background: Althaea officinalis L., Lavandula angustifolia Mill. and Rosa x damascena Herrm. are used to treat excision or burn lesions in traditional medicine. Objective: To evaluate the healing effect of a polyherbal ointment containing L. angustifolia, R. x damascena, and A. officinalis combination on wounds caused by third grade skin cut. Methods: To evaluate the wound healing effect of this polyherbal formulation, an ointment containing the extract of A. officinalis, the essential oil of R. x damascena (2 % essence) and the essential oil of L. officinalis (2 % essence) in an eucerin base (20:20:10:50) was prepared. The formulation was examined using excision wound test for 14 days and the percentage of healing effect in each group was calculated. Formalin induced rat hind paw edema method was used for determination of anti-inflammatory activities of the same formulation. Results: The percentage of recovery in the polyherbal formulation group was significantly higher than the other groups. Histological studies also confirmed these results. Herbal formulation treated group showed significant improvement in terms of re-epithelialization, angiogenesis, collagen deposition, and reducing inflammation. The percentage of wound healing was 99.07 ± 0.34, 99.22 ± 0.35, 98.45 ± 0.733, 98.75 ± 0.88 and 63.72 ± 5.64, for the polyherbal formulation, L. angustifolia, R. damascena, A. officinalis extracts, and the placebo group, respectively. Conclusion: This polyherbal formulation could act as a wound healing agent, possibly due to the suppression of inflammatory mediators. The presence of phenolic compounds might be a reason behind the therapeutic effect of these plants.
Mohsen Naseri, Maryam Iranzadasl, Farzaneh Ghaffari, Vahedeh Naseri, Fatemeh Emadi, Fatemeh Alijaniha, Abdolazim Behfar, Zahra Bahaeddin,
year 21, Issue 84 (12-2022)
Abstract
Background: Dandruff is a common and important scalp disorder affecting almost half of the population at the pre-pubertal age. Various studies have shown that using herbs can be a good option for improving dandruff. There is a way to search for effective herbal and natural remedies that, in addition to being scientifically valid, is a quick path in the process of discovering, designing, and obtaining natural remedies. This method is based on the knowledge of traditional medicine. Persian medicine contains vast knowledge in diagnosing, preventing and treating diseases, including valuable information from the experiences of scientists. Objective: This article introduces materia medica mentioned in Persian medicine used for dandruff. Methods: The present study is a review based on the study of traditional Persian medicine books. Hereon, effective drugs for improving dandruff mentioned in the second volume of Ibn Sina's book (Al Qanun-fi al-Tibb) have been listed, and then four reference books of traditional medicine (al-Abnieh an Haghayegh al-advieh, Tazkare Ulul Al-bab, Tohfat al-Momenin, and Makhzan al-Advieh) are reviewed. Results: Twenty-one materia medica were found as anti-dandruff remedies. Based on the scores, Trigonella foenum-graecum L. and Beta vulgaris L. earned the best points respectively, and Prunus amygdalus Batsch, Sesamum indicum L., Ziziphus spina-christi (L.) Desf., and sodium tetraborate decahydrate got the next orders with equal scores. Conclusion: The list of drugs collected in this study can be considered as a basis for further studies to design and make new effective drugs for treating dandruff.
Parisa Salari, Mansour Ghaffari Moghaddam, Masoumeh Bahreini, Mohammad Reza Sharifmoghadam,
year 22, Issue 86 (5-2023)
Abstract
Background: Foeniculum vulgare Mill. seeds contain polyphenolic compounds which can be considered a suitable option for the green synthesis of nanoparticles. Objective: In this study, the antibacterial activity against foodborne bacteria of ZnO nanoparticles synthesized from the aqueous extract of Foeniculum vulgare Mill. seeds was evaluated. Methods: The synthesized ZnO nanoparticles were characterized using different analyses. The minimum inhibitory and bactericidal concentration of the nanoparticles were investigated against standard foodborne bacteria, S. aureus, Y. enterocolitica, E. coli O157:H7, and B. cereus, using the broth microdilution method. Results: UV-Vis spectroscopy analysis indicated an absorption peak at 231 nm which confirms the formation of ZnO nanoparticles. In addition, the X-ray diffraction pattern is consistent with the JCPDS cards, which also means the formation of ZnO nanoparticles. The results of the electron microscope revealed that the nanoparticles had a hexagonal shape with an average size of 50 nm, which is in agreement with the results obtained from the dynamic light scattering analysis. In addition, the minimum inhibitory concentration of ZnO nanoparticles against gram-negative and gram-positive bacteria, Y. enterocolitica, E. coli O157:H7, S. aureus, and B. cereus were 62.5, 62.5, 31.25 and 500 µg/ml, respectively. Conclusion: ZnO nanoparticles synthesized from Foeniculum vulgare Mill. seed extract had an appropriate antibacterial effect against foodborne bacteria.
