سال 22، شماره 87 - ( 9-1402 )
سال 22 شماره 87 صفحات 56-39 |
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Babaie M, Cheniany M, Ganjeali A, Vaezi J. Quantitative HPLC analysis of flavonoids and evaluation of antioxidant activity in two organs of Salvia tebesana Bunge. J. Med. Plants 2023; 22 (87) :39-56
URL: http://jmp.ir/article-1-3551-fa.html
URL: http://jmp.ir/article-1-3551-fa.html
بابایی مریم، چنیانی منیره، گنجعلی علی، واعظی جمیل. ارزیابی کمی فلاونوئیدها با استفاده از تکنیک HPLC و ارزیابی فعالیت آنتی اکسیدانی در دو اندام Salvia tebesana Bunge. فصلنامه گياهان دارویی. 1402; 22 (87) :39-56
مریم بابایی1 
، منیره چنیانی* 2، علی گنجعلی1 ، جمیل واعظی1
، منیره چنیانی* 2، علی گنجعلی1 ، جمیل واعظی1
1- گروه زیستشناسی، دانشکده علوم، دانشگاه فردوسی مشهد، ایران
2- گروه زیستشناسی، دانشکده علوم، دانشگاه فردوسی مشهد، ایران ،cheniany@um.ac.ir
2- گروه زیستشناسی، دانشکده علوم، دانشگاه فردوسی مشهد، ایران ،
چکیده: (1285 مشاهده)
مقدمه: مریم‌گلی به عنوان گیاهی مهم در طب‌سنتی، در نقاط محدودی از خاورمیانه پراکنده شده است. هدف: در این مطالعه سه حلال قطبی متانول 80 درصد، اتانول 80 درصد و آب دوبار تقطیرشده برای ترغیب ترکیبات فنلی Salvia tebesana مورد بررسی قرار گرفت. روش بررسی: در ابتدا فعالیت آنتی‌اکسیدانی (برآوردشده با ظرفیت آنتی‌اکسیدانی کل DPPH و سنجش مهار رادیکال FRAP) و محتوای فنل، ارتودیفنل، اسیدهای‌فنلی، فلاونوئید، فلاونول و پروآنتوسیانیدین عصاره‌های برگ و ساقه ارزیابی شدند. پس از انتخاب بهترین حلال، نمونه‌ها از نظر ترکیبات فلاونوئیدی مجزا (اپیژنین، کوئرستین، میریستین و روتین) توسط HPLC-PDA مورد سنجش قرار گرفتند. نتایج: عصاره‌های گیاهی مختلف فعالیت‌های قوی درمهار رادیکال‌ها نشان دادند و عصاره برگ به‌دست‌آمده با متانول 80 درصد، بیشترین ظرفیت آنتی‌اکسیدانی را نشان داد. درهمین عصاره بیشترین محتوای ارتودیفنل، اسیدهای فنلی، فلاونوئید و پروآنتوسیانیدین به ثبت رسید (به ترتیب 46/2 ± 1598/5، 41/3 ± 742/2، 21/9 ± 487/6 و 31/4 ± 350/1 میلیگرم در 100 گرم ماده خشک) درحالیکه عصاره اتانولی برگ بیشترین محتوای فنل و فلاونوئید را به خود اختصاص داد (به ترتیب 47/3 ± 2299/2 و 42/3 ± 359/1 میلیگرم در 100 گرم ماده خشک). سطح بیشینه فلاون اپیژنین (11/1 ± 466/1 میکروگرم در گرم ماده خشک) و فلاونول روتین (10/02 ± 348/6 میکروگرم در گرم ماده خشک) نیز در عصاره متانولی برگ یافت و برای اولین بار در S. tebesana گزارش شد. نتیجه‌گیری: این یافته‌ها تضمین می‌کند که S. tebesana، به‌ویژه برگ‌ها، می‌تواند به عنوان یک آنتی‌اکسیدان طبیعی و در جهت کاهش تنش اکسیداتیو، در برنامه‌های غذایی و پزشکی پیشنهاد شود.
نوع مطالعه: پژوهشی |
موضوع مقاله:
گياهان دارویی
دریافت: 1402/5/29 | پذیرش: 1402/9/8 | انتشار: 1402/10/10
دریافت: 1402/5/29 | پذیرش: 1402/9/8 | انتشار: 1402/10/10
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