year 19, Issue 73 (3-2020)                   J. Med. Plants 2020, 19(73): 82-90 | Back to browse issues page

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Amraee S, Bahramikia S, Mohammadi A. Effective fraction of Teucrium polium suppressed polyol pathway through inhibiting the aldose reductase enzyme: strategy to reduce retinopathy. J. Med. Plants 2020; 19 (73) :82-90
1- Department of Biology, Faculty of Science, Lorestan University, Khorramabad, Iran
2- Department of Biology, Faculty of Science, Lorestan University, Khorramabad, Iran ,
Abstract:   (1800 Views)
Background: Several metabolic pathways are involved in the complications of diabetes like polyol pathway. Aldose reductase (AR) is a key enzyme in the polyol pathway, which catalyzes the conversion of glucose to sorbitol. AR inhibitors are appropriate to prevent and treat the diabetes complications. Objective: This study was designed to investigate the effect of different fractions of Teucrium polium on the AR activity. Methods: Fifty cow’s eye lenses were prepared and AR enzyme was purified according to the Hyman-Kinoshita method. The enzyme activity in the presence of the crude extract and different fractions of Teucrium polium (1, 5, 10, 20 and 100 μg/ml) was measured. In addition, IC50 content of fractions was also measured for the neutralization of DPPH free radical. Since some AR inhibitors are phenolic compounds, the phenolic and flavonoid contents have been investigated. Results: Results showed that the highest phenol and flavonoid content and the lowest IC50 value (3.67 µg/ml) for AR inhibition were related to the ethyl acetate fraction. Line weaver-Burk plot showed that ethyl acetate fraction acts as a non-competitive enzyme inhibition. Conclusion: Thus, T. polium can be proposed as a therapy to prevent or treat chronic complications of diabetes in the future.
Full-Text [PDF 365 kb]   (879 Downloads)    
Type of Study: Research | Subject: Pharmacognosy & Pharmaceutics
Received: 2019/04/8 | Accepted: 2019/07/10 | Published: 2020/06/6

1. American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 2014; 37 (Supplement 1), S81-S90. DOI: 10.2337/dc14-S081. [DOI:10.2337/dc14-S081]
2. Bahmani F, Athaie SZ, Aldavood SJ and Ghahghaei A. Glycine therapy inhibits the progression of cataract in streptozotocininduced diabetic rats. Molecular Vision 2012; 18: 439. [PMID: 22355255].
3. Yabe-Nishimura C. Aldose reductase in glucose toxicity: a potential target for the prevention of diabetic complications. Pharmacol. Rev. 1998; 50 (1): 21-34. [PMID: 9549756].
4. Tang WH, Martin KA and Hwa J. Aldose reductase, oxidative stress, and diabetic mellitus. Front Pharmacol. 2012; 3: 87. DOI: 10.3389/fphar.2012.00087. [DOI:10.3389/fphar.2012.00087]
5. Rakowitz D, Maccari R, Ottanà R and Vigorita MG. In vitro aldose reductase inhibitory activity of 5-benzyl-2, 4-thiazolidinediones. Bioorg. Med. Chem. 2006; 14 (2): 567-574. DOI: 10.1016/j.bmc.2005.08.056. [DOI:10.1016/j.bmc.2005.08.056]
6. Lee YS, Kim SH, Jung SH, Kim JK, Pan CH and Lim SS. Aldose reductase inhibitory compounds from Glycyrrhiza uralensis. Biol. Pharm. Bull. 2010; 33 (5): 917-921. [PMID: 20460778]. [DOI:10.1248/bpb.33.917]
7. Clark JCM and Lee DA. Prevention and treatment of the complications of diabetes mellitus. N. Engl. J. Med. 1995; 332 (18): 1210-1217. DOI: 10.1056/NEJM199505043321807. [DOI:10.1056/NEJM199505043321807]
8. Kim TH, Kim JK, Kang YH, Lee JY, Kang IJ and Lim SS. Aldose reductase inhibitory activity of compounds from Zea mays L. Biomed Res. Int. 2013; 2013: 1-8. DOI: 10.1155/2013/727143. [DOI:10.1155/2013/727143]
9. Hashim Z and Zarina S. Osmotic stress induced oxidative damage: possible mechanism of cataract formation in diabetes. J. Diabetes Complications 2012; 26: 275-9. DOI: 10.1016/j.jdiacomp.2012.04.005. [DOI:10.1016/j.jdiacomp.2012.04.005]
10. Tomlinson DR, Stevens EJ and Diemel LT. Aldose reductase inhibitors and their potential for the treatment of diabetic complications. Trends Pharmacol. Sci. 1994; 15 (8): 293-297. [PMID: 7940997]. [DOI:10.1016/0165-6147(94)90010-8]
11. Singh Grewal A, Bhardwaj S, Pandita D, Lather V and Singh Sekhon B. Updates on aldose reductase inhibitors for management of diabetic complications and non-diabetic diseases. Mini Rev. Med. Chem. 2016; 16 (2): 120-162. [PMID: 26349493]. [DOI:10.2174/1389557515666150909143737]
12. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M and Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Bio. 2007; 39 (1): 44-84. DOI: 10.1016/j.biocel.2006.07.001. [DOI:10.1016/j.biocel.2006.07.001]
13. Lean ME, Noroozi M, Kelly I, Burns J, Talwar D, Sattar N and Crozier A. Dietary flavonols protect diabetic human lymphocytes against oxidative damage to DNA. Diabetes 1999; 48 (1): 176-181. [PMID: 9892240]. [DOI:10.2337/diabetes.48.1.176]
14. Bahramikia S and Yazdanparast R. Phytochemistry and medicinal properties of Teucrium polium L. (Lamiaceae). Phytother. Res. 2012; 26 (11): 1581-1593. DOI: 10.1002/ptr.4617. [DOI:10.1002/ptr.4617]
15. Varma SD, Mikuni I and Kinoshita JH. Flavonoids as inhibitors of lens aldose reductase. Science 1975; 188 (4194): 1215-1216. [PMID: 1145193]. [DOI:10.1126/science.1145193]
16. Park H.Y., Kim H.K., Jeon S.H. and et al. Aldose reductase inhibitors from the leaves of Salix hulteni. J. Korean Soc. Appl. Biol. Chem. 2009; 52: 493. DOI: org/10.3839/jksabc.2009.084 [DOI:10.3839/jksabc.2009.084]
17. Suryanarayana P, Pasupulati, Anil Kumar, Saraswat, Megha, Petrash, Mark and Reddy G. Inhibition of aldose reductase by tannoid principles of Emblica officinalis: Implications for the prevention of sugar cataract. Molecular Vision 2004; 10: 148-54. [PMID: 15031705].
18. Suzen S and Buyukbingol E. Recent studies of aldose reductase enzyme inhibition for diabetic complications. Curr. Med. Chem. 2003; 10 (15): 1329-1352. [PMID: 12871133]. [DOI:10.2174/0929867033457377]
19. Lu Q, Hao M, Wu W, Zhang N, Isaac AT, Yin J, Zhu X, Du L and Yin X. Antidiabetic cataract effects of GbE, rutin and quercetin are mediated by the inhibition of oxidative stress and polyol pathway. Acta Biochim. Pol. 2018; 65 (1): 35-41. DOI: 10.18388/abp.2016_1387. [DOI:10.18388/abp.2016_1387]
20. Nosrati N, Aghazadeh S and Yazdanparast R. Effects of Teucrium polium on insulin resistance in nonalcoholic steatohepatitis. J. Acupunct. Meridian Stud. 2010; 3 (2): 104-110. DOI: 10.1016/S2005-2901(10)60019-2. [DOI:10.1016/S2005-2901(10)60019-2]
21. Esmaeili MA and Yazdanparast R. Hypoglycaemic effect of Teucrium polium: studies with rat pancreatic islets. J. Ethnopharmacol. 2004; 95: 27-30. DOI: 10.1016/j.jep.2004.06.023. [DOI:10.1016/j.jep.2004.06.023]
22. Ardestan A and Yazdanparast R. Inhibitory effects of ethyl acetate extract of Teucrium polium on in vitro protein glycoxidation. Food Chem. Toxicol. 2007; 45: 2402-2411. DOI: 10.1016/j.fct.2007.06.020. [DOI:10.1016/j.fct.2007.06.020]
23. Slinkard K and Singleton VL. Total phenol analysis: automation and comparison with manual methods. Am. J. Enol. Vitic. January. 1977; 28 (1): 49-55.
24. Zhishen J, Mengcheng T and Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects onsuperoxide radicals. Food Chem. 1999; 64 (4): 555-559. DOI: 10.1016/S0308-8146(98)00102-2. [DOI:10.1016/S0308-8146(98)00102-2]
25. Bahramikia S and Yazdanparast R. Antioxidant Efficacy of Nasturtium officinale Extracts Using Various In Vitro Assay Systems. JAMS. 2010; 3 (4): 283-290. (10) 60049-0. [DOI:10.1016/S2005-2901(10)60049-0]
26. Hayman S and Kinoshita JH. Isolation and properties of lens aldose reductase. J. Biol. Chem. 1965; 240 (2): 877-882. [PMID: 14275148].
27. Lee EH, Song DG, Lee JY, Pan CH, Um BH and Jung SH. Inhibitory effect of the compounds isolated from Rhus verniciflua on aldose reductase and advanced glycation end products. Biol. Pharm. Bull. 2008; 31 (8): 1626-1630. [PMID: 18670102]. [DOI:10.1248/bpb.31.1626]
28. Yoon HN, Lee MY, Kim JK, Suh HW and Lim SS. Aldose reductase inhibitory compounds from Xanthium strumarium. Arch. Pharm. Res. 2013; 36 (9): 1090-1095. DOI: 10.1007/s12272-013-0123-5. [DOI:10.1007/s12272-013-0123-5]
29. Lee YS, Kang YH, Jung JY, Kang IJ, Han SN, Chung JS and et al. Inhibitory constituents of aldose reductase in the fruiting body of Phellinus linteus. Biol. Pharm Bull. 2008; 31 (4): 765-768. [PMID: 18379080]. [DOI:10.1248/bpb.31.765]
30. Pollreisz A and Schmidt-Erfurth U. Diabetic cataract-pathogenesis, epidemiology and treatment. J. Ophthalmol. 2010; 2010: 1-8. DOI: 10.1155/2010/608751. [DOI:10.1155/2010/608751]
31. Ruhe RC and McDonald RB. Use of antioxidant nutrients in the prevention and treatment of type 2 diabetes. J. Am. Coll. Nutr. 2001; 20 (Sup 5): 363S-369S. [PMID: 11603645]. [DOI:10.1080/07315724.2001.10719169]

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