year 24, Issue 94 (7-2025)
J. Med. Plants 2025, 24(94): 19-30 |
Back to browse issues page
Ethics code: 1256/KHTN-ACUCUS
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Nguyen Thi Thanh N, Nguyen Minh C, Nguyen Thi My T. Wound healing promoting activities of Dimetia capitellata (Wall. ex G.Don) Neupane & N.Wikstr. leaves. J. Med. Plants 2025; 24 (94) :19-30
URL: http://jmp.ir/article-1-3716-en.html
URL: http://jmp.ir/article-1-3716-en.html
1- Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Vietnam National University, Ho Chi Minh City, Vietnam
2- Laboratory of Molecular Biotechnology, University of Science, Vietnam National University, Ho Chi Minh City, Vietnam
3- Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Vietnam National University, Ho Chi Minh City, Vietnam ,ntmtrinh@hcmus.edu.vn
2- Laboratory of Molecular Biotechnology, University of Science, Vietnam National University, Ho Chi Minh City, Vietnam
3- Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Vietnam National University, Ho Chi Minh City, Vietnam ,
Abstract: (119 Views)
Background: The leaves of Dimetia capitellata (Wall. ex G.Don) Neupane & N.Wikstr. have been used as a traditional medicine to treat skin wounds in some human communities. Objective: This study aimed to evaluate the wound healing activities of the 70% ethanolic extract of this plant’s leaves (DcEE) and its ethyl acetate (DcEAF), petroleum ether (DcPEF), and water (DcWF) fractions. Methods: The nitric oxide levels in LPS-activated RAW 26.7 cells were measured by Griss assay. The proliferation and migration of cells were assessed by MTT and scratchings methods, respectively. In an in vivo assay, skin wounds were created in mice and then daily treated with DcEE, and the wound contraction percentages were determined every day. The mRNA levels of inflammation-related genes were evaluated using qRT-PCR. Results: We found that the DcEE contained 74.7 ± 2.9 mgGAE/gE polyphenols and 66.5 ± 2.8 mgQE/gE flavonoids, and showed a weak antioxidant activity. The DcEE treatment also reduced the in vitro nitric oxide level released from LPS-stimulated RAW 26.7 macrophages by 89.8±8.2% and decreased the mRNA levels of inflammation-related genes NF-κB1 and TNF-α in wound tissues of the examined mice, suggesting its anti-inflammatory activity. Besides, DcEE stimulated fibroblast and keratinocyte proliferation and induced keratinocyte migration. Importantly, in the in vivo assay, the wound closure time in DcEE-treated mice was reduced approximately 2 days in comparison with that in the control mice. Conclusions: These findings provided some evidence to prove the traditional utilization of D. capitellata leaves in skin wound treatment.
Keywords: Anti-bacterial activity, Cell migration, Cell proliferation, Nitric oxide inhibitory, Wound healing, Dimetia capitellata
Type of Study: Research |
Subject:
Medicinal Plants
Received: 2024/08/1 | Accepted: 2025/05/26 | Published: 2025/07/1
Received: 2024/08/1 | Accepted: 2025/05/26 | Published: 2025/07/1
References
1. Saeidnia S, Gohari AR, Mokhber-Dezfuli N and Kiuchi F. A review on phytochemistry and medicinal properties of the genus Achillea. DARU. 2011; 19(3): 173-186.
2. Yan-Jiao D, Guo-Ying Z, Xiao-Yan H, Gen-Chun W and Jun H. Screening of antibacterial activity of 20 Chinese herbal medicines in Yunnan. Afr. J. Pharm. Pharmacol. 2013; 7(44): 2859-65. [DOI:10.5897/AJPP2012.1513]
3. Ahmad R, Ali AM, Israf DA, Ismail NH, Shaari K and Lajis NH. Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species. Life Sciences 2005; 76(17): 1953-64. [DOI:10.1016/j.lfs.2004.08.039]
4. WFO: Dimetia capitellata (Wall. ex G.Don) Neupane & N.Wikstr. Published on the Internet: https://www.worldfloraonline.org/taxon/wfo-0001342523. Accessed 2025.
5. Wiart C, Mogana S, Khalifah S, Mahan M, Ismail S, Buckle M, Narayana AK and Sulaiman M. Antimicrobial screening of plants used for traditional medicine in the state of Perak, Peninsular Malaysia. Fitoterapia 2004; 75(1): 68-73. [DOI:10.1016/j.fitote.2003.07.013]
6. Phuong NM, Van Sung T, Porzel A, Schmidt J and Adam G. Two new β-carboline alkaloids from Hedyotis capitellata var. mollis. Planta Medica 1999; 65(08): 761-2. [DOI:10.1055/s-2006-960861]
7. Ahmad R, Shaari K, Lajis NH, Hamzah AS, Ismail NH and Kitajima M. Anthraquinones from Hedyotis capitellata. Phytochem. 2005; 66(10): 1141-7. [DOI:10.1016/j.phytochem.2005.02.023]
8. Ngoc TTB, Nga NTH, Trinh NTM, Thuoc TL and Thao DTP. Elephantopus mollis Kunth extracts induce antiproliferation and apoptosis in human lung cancer and myeloid leukemia cells. J. Ethnopharmacol. 2020; 263: 113222. [DOI:10.1016/j.jep.2020.113222]
9. Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S and Ju Y-H. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J. Food and Drug Anal. 2014; 22(3): 296-302. [DOI:10.1016/j.jfda.2013.11.001]
10. Yakaew S, Itsarasook K, Ngoenkam J, Jessadayannamaetha A, Viyoch J and Ungsurungsie M. Ethanol extract of Terminalia chebula fruit protects against UVB-induced skin damage. Pharmaceutical Biology 2016; 54(11): 2701-7. [DOI:10.1080/13880209.2016.1179768]
11. Pomari E, Stefanon B and Colitti M. Effect of plant extracts on H2O2-induced inflammatory gene expression in macrophages. JIR. 2014; 103-12. [DOI:10.2147/JIR.S61471]
12. Budovsky A, Yarmolinsky L and Ben‐Shabat S. Effect of medicinal plants on wound healing. Wound Repair and Regen. 2015; 23(2): 171-83. [DOI:10.1111/wrr.12274]
13. Ghosh PK and Gaba A. Phyto-extracts in wound healing. J. Pharm. Pharm. Sci. 2013; 16(5): 760-820. [DOI:10.18433/J3831V]
14. Goldberg MT, Han Y-P, Yan C, Shaw MC and Garner WL. TNF-α suppresses α-smooth muscle actin expression in human dermal fibroblasts: an implication for abnormal wound healing. J. Invest. Dermatol. 2007; 127(11): 2645-55. [DOI:10.1038/sj.jid.5700890]
15. Yip HY, Poh MSW and Chia YY. The effects of glycyrrhizic acid and glabridin in the regulation of CXCL5 inflammation gene on acceleration of wound healing. Asian Pacific Journal of Tropical Biomedicine 2016; 6(2): 108-13. [DOI:10.1016/j.apjtb.2015.10.009]
16. Kuonen R, Weissenstein U, Urech K, Kunz M, Hostanska K, Estko M, Heusser P and Baumgartner S. Effects of lipophilic extract of Viscum album L. and oleanolic acid on migratory activity of NIH/3T3 fibroblasts and on HaCat keratinocytes. Evid Based Complement. Alternat. Med. 2013; 2013: 718105. [DOI:10.1155/2013/718105]
17. Ho JK and Hantash BM. The principles of wound healing. Expert Review of Dermatol. 2013; 8(6): 639-58. [DOI:10.1586/17469872.2013.857161]
18. Barrientos S, Stojadinovic O, Golinko MS, Brem H and Tomic-Canic M. Growth factors and cytokines in wound healing. Wound Repair and Regeneration 2008; 16(5): 585-601. [DOI:10.1111/j.1524-475X.2008.00410.x]
19. Pereira SG and Oakley F. Nuclear factor-κB1: Regulation and function. The International Journal of Biochemistry & Cell Biology 2008; 40(8): 1425-30. [DOI:10.1016/j.biocel.2007.05.004]
20. Newton K and Dixit VM. Signaling in innate immunity and inflammation. Cold Spring Harbor Perspectives in Biology 2012; 4(3): a006049. [DOI:10.1101/cshperspect.a006049]
21. Nhung TTP and Quoc LPT. Investigation of the Inflammatory, Antipyretic, and Analgesic Potential of Ethanol Extract from Hedyotis capitellata Wall. ex G. Don Leaves in Mice. Tropical Journal of Natural Product Research 2023; 7(12). [DOI:10.26538/tjnpr/v7i12.20]
22. Zulkefli N, Che Zahari CNM, Sayuti NH, Kamarudin AA, Saad N, Hamezah HS, Bunawan H, Baharum SN, Mediani A, Ahmed QU, Ismail AFH and Sarian MN. Flavonoids as potential wound-healing molecules: Emphasis on pathways perspective. IJMS. 2023; 24(5): 4607. [DOI:10.3390/ijms24054607]
23. Aslam MS, Ahmad MS, Riaz H, Raza SA, Hussain S, Qureshi OS, Maria P, Hamzah Z and Javed O. Role of Flavonoids as Wound Healing Agent. In: Toshiki A, Md A, editors. Phytochemicals. Rijeka: IntechOpen; 2018, p. Ch. 6. [DOI:10.5772/intechopen.79179]
24. Melguizo-Rodríguez L, de Luna-Bertos E, Ramos-Torrecillas J, Illescas-Montesa R, Costela-Ruiz VJ and García-Martínez O. Potential effects of phenolic compounds that can be found in olive oil on wound healing. Foods 2021; 10(7): 1642. [DOI:10.3390/foods10071642]
25. Machado GHA, Marques TR, De Carvalho TCL, Duarte AC, De Oliveira FC, Gonçalves MC, Piccoli RH and Correa AD. Antibacterial activity and in vivo wound healing potential of phenolic extracts from jaboticaba skin. Chemical Biology & Drug Design. 2018; 92(1): 1333-43. [DOI:10.1111/cbdd.13198]
26. Li C-Q, Pang B, Kiziltepe T, Trudel LJ, Engelward BP, Dedon PC and Wong GN. Threshold effects of nitric oxide-induced toxicity and cellular responses in wild-type and p53-null human lymphoblastoid cells. Chem. Res. Toxicol. 2006; 19(3): 399-406. [DOI:10.1021/tx050283e]
27. Dedon PC and Tannenbaum SR. Reactive nitrogen species in the chemical biology of inflammation. Arch. Biochem. Biophys. 2004; 423(1): 12-22. [DOI:10.1016/j.abb.2003.12.017]
28. Lucas L, Russell A and Keast R. Molecular mechanisms of inflammation. Anti-inflammatory benefits of virgin olive oil and the phenolic compound oleocanthal. Curr. Pharm. Des. 2011; 17(8): 754-68. [DOI:10.2174/138161211795428911]
29. Rafi MM, Yadav PN and Rossi AO. Glucosamine inhibits LPS‐induced COX‐2 and iNOS expression in mouse macrophage cells (RAW 264.7) by inhibition of p38‐MAP kinase and transcription factor NF‐κB. Molecular Nutrition & Food Res. 2007; 51(5): 587-93. [DOI:10.1002/mnfr.200600226]
30. Choi RJ, Ngoc TM, Bae K, Cho H-J, Kim D-D, Chun J, Khan S and Kim YS. Anti-inflammatory properties of anthraquinones and their relationship with the regulation of P-glycoprotein function and expression. Europ. J. Pharmaceutical Sci. 2013; 48(1-2): 272-81. [DOI:10.1016/j.ejps.2012.10.027]
31. Ngoc PB, Pham TB, Nguyen HD, Tran TT, Chu HH, Chau VM, Lee J-H and Nguyen TD. A new anti-inflammatory β-carboline alkaloid from the hairy-root cultures of Eurycoma longifolia. Natural Product Research 2016; 30(12): 1360-5. [DOI:10.1080/14786419.2015.1056187]
32. Woodley DT, O'Keefe EJ and Prunieras M. Cutaneous wound healing: A model for cell-matrix interactions. J. Am. Acad. Dermatol. 1985; 12(2): 420-33. [DOI:10.1016/S0190-9622(85)80005-0]
33. Mimura Y, Ihn H, Jinnin M, Asano Y, Yamane K and Tamaki K. Epidermal growth factor induces fibronectin expression in human dermal fibroblasts via protein kinase C δ signaling pathway. J. Invest. Dermatol. 2004; 122(6): 1390-8. [DOI:10.1111/j.0022-202X.2004.22618.x]
34. Gurtner GC, Werner S, Barrandon Y and Longaker MT. Wound repair and regeneration. Nature 2008; 453: 314-21. [DOI:10.1038/nature07039]
35. Chen X, Zhang M, Chen S, Wang X, Tian Z, Chen Y, Xu P, Zhang L, Zhang L and Zhang L.. Peptide-modified chitosan hydrogels accelerate skin wound healing by promoting fibroblast proliferation, migration, and secretion. Cell Transplantation 2017; 26(8): 1331-40. [DOI:10.1177/0963689717721216]
36. Gerber PA, Buhren BA, Schrumpf H, Homey B, Zlotnik A and Hevezi P. The top skin-associated genes: a comparative analysis of human and mouse skin transcriptomes. Biol. Chem. 2014; 395(6): 577-91. [DOI:10.1515/hsz-2013-0279]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
