year 18, Issue 70 (5-2019)                   J. Med. Plants 2019, 18(70): 97-109 | Back to browse issues page

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Asghari B, Asghari B, Ghorbani Nohooji M. Persian Thyme (Thymus persicus): A Plant Containing Active Metabolites with Antioxidant, Anti-diabetic and Anti-Alzheimer Effects. J. Med. Plants. 2019; 18 (70) :97-109
1- Department of Horticultural Sciences Engineering, Agriculture and Natural Resources Faculty, Imam Khomeini International University, Qazvin, Iran ,
2- Department of Horticultural Sciences Engineering, Agriculture and Natural Resources Faculty, Imam Khomeini International University, Qazvin, Iran
3- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
Abstract:   (2232 Views)
Background: Thymus persicus is one of the Iranian endemic medicinal plants, that its decoction and various products have plenty of food and pharmaceutical uses.
Objective: Evaluation of T. persicus products metabolite contains and determination of the best antioxidant, anti-diabetic and anti-Alzheimer extract.
Methods: Samples total phenolic, flavonoid and tannin contents were determined by Folin-Ciocalteu, AlCl3, Folin-Denis reagents and for saponin content vanillin-sulfuric acid method were used. To the investigation of products antioxidant effect, DPPH and OH radical scavenging and phosphomolybdenum and ferrous ion chelating methods were employed. To evaluate of samples anti-diabetic properties α-amylase and α-glucosidase inhibitory effects, and to investigate of anti-Alzheimer potential, acetyl and butyryl cholinesterase inhibitory effect were measured.
Results: Decoction and hydroalcoholic extract of T. persicus showed the highest phenol, flavonoid and tannin contents in comparison with the others. Ethyl acetate extract had the highest saponin content. According to the antioxidant assays, decoction and hydroalcoholic extract exhibited the best potential. In anti-diabetic (inhibition of α-amylase and α-glucosidase enzymes) and anti-Alzheimer (inhibition of acetyl and butyryl cholinesterase) assays, decoction and hydroalcoholic extract showed significantly higher power.
Conclusion: The high ability of decoction and hydroalcoholic extract of T. persicus in antioxidant, anti-diabetic and anti-Alzheimer can be related to their high phenol, flavonoid and tannin content.
Full-Text [PDF 915 kb]   (668 Downloads)    
Type of Study: Research | Subject: Medicinal Plants
Received: 2018/09/23 | Accepted: 2018/12/17 | Published: 2019/05/26

1. Sonboli A, Mirjalili MH, Bakhtiar ZIBA and Jamzad ZIBA. Molecular authentication of Thymus persicus based on nrDNA ITS sequences data. Iran. J. Bot. 2013; 19 (2): 179-85.
2. Nickavar B, Mojab F and Dolat-Abadi R. Analysis of the essential oils of two Thymus species from Iran. Food Chem. 2005; 90 (4): 609-11. [DOI:10.1016/j.foodchem.2004.04.020]
3. Figueiredo AC, Barroso JG, Pedro LG, Salgueiro L, Miguel MG and Faleiro ML. Portuguese Thymbra and Thymus species volatiles: chemical composition and biological activities. Curr. Pharm. Des. 2008; 14 (29): 3120-40. [DOI:10.2174/138161208786404218]
4. Hosseinzadeh S, Jafarikukhdan A, Hosseini A and Armand R. The application of medicinal plants in traditional and modern medicine: a review of Thymus vulgaris. Int. J. Clin. Med. 2015; 6 (09): 635-42. [DOI:10.4236/ijcm.2015.69084]
5. Taghizadeh Saroukolai A, Moharramipour S, and Meshkatalsadat MH. Insecticidal properties of Thymus persicus essential oil against Tribolium castaneum and Sitophilus oryzae. J. Pest. Sci. 2010; 83 (1): 3-8. [DOI:10.1007/s10340-009-0261-1]
6. Stahl-Biskup, E. The chemical composition of Thymus oils: a review of the literature 1960-1989. J. Essent. Oil Res. 1991; 3 (2): 61-82. [DOI:10.1080/10412905.1991.9697915]
7. Rasooli I, and Mirmostafa SA. Bacterial susceptibility to and chemical composition of essential oils from Thymus kotschyanus and Thymus persicus. J. Agric. Food Chem. 2003; 51 (8): 2200-5. [DOI:10.1021/jf0261755]
8. Boros B, Jakabová S, Dörnyei Á, Horváth G, Pluhár Z, Kilár F, and Felinger A. Determination of polyphenolic compounds by liquid chromatography-mass spectrometry in Thymus species. J. Chromatogr A. 2010; 1217 (51): 7972-80. [DOI:10.1016/j.chroma.2010.07.042]
9. Mirjalili MH., Ayyari M, Bakhtiar Z, Moridi Farimani M and Sonboli, A. Quantification of betulinic, oleanolic and ursolic acids as medicinally important triterpenoids in some Thymus species from Iran. Res. J. Pharmaco. 2016; 3 (1): 23-8.
10. Hyun TK, Kim HC and Kim JS. Antioxidant and antidiabetic activity of Thymus quinquecostatus Celak. Ind. Crops Prod. 2014; 52: 611-6. [DOI:10.1016/j.indcrop.2013.11.039]
11. Kindl M, Blazekovic B, Bucar F and Vladimir-Knezevic S. Antioxidant and anticholinesterase potential of six Thymus species. J. Evid. Based Complementary Altern. Med. 2015; Article ID 403950, 10 pages. [DOI:10.1155/2015/403950]
12. Zengin G, Atasagun B, Aumeeruddy MZ, Saleem H, Mollica A, Bahadori MB and Mahommodally MF. Phenolic profiling and in vitro biological properties of two Lamiaceae species (Salvia modesta and Thymus argaeus): A comprehensive evaluation. Ind. Crops Prod. 2019; 128: 308-14. [DOI:10.1016/j.indcrop.2018.11.027]
13. Da Silva JAT. Thymus Persicus (Poniger ex reach. F.) Jalas. CIBTech J. Biotech. 2016; 5 (3): 24-7.
14. Sefidkon F, Dabiri M and Mirmostafa, SA. The essential oil of Thymus persicus (Ronniger ex Rech. f.) Jalas from Iran. J. Essent. Oil Res. 2002; 14 (5): 351-2. [DOI:10.1080/10412905.2002.9699880]
15. Safaei-Ghomi J, Meshkatalsadat MH, Shamai S, Hasheminejad M and Hassani A. Chemical characterization of bioactive volatile molecules of four Thymus species using nanoscale injection method. Dig. J. Nanomater Biostruct. 2009; 4 (4): 835-41.
16. Rasooli I and Mirmostafa SA. Bacterial susceptibility to and chemical composition of essential oils from Thymus kotschyanus and Thymus persicus. J. Agric. Food Chem. 2003; 51 (8): 2200-5. [DOI:10.1021/jf0261755]
17. Talei GR and Meshkatalsadat MH. Antibacterial activity and chemical constitutions of essential oils of Thymus persicus and Thymus eriocalyx from west of Iran. Pak. J. Biol. Sci. 2007; 10 (21): 3923-6. [DOI:10.3923/pjbs.2007.3923.3926]
18. Bahadori MB, Valizadeh H, Asghari B, Dinparast L, Bahadori S and Moridi Farimani M. Biological activities of Salvia santolinifolia Boiss. A multifunctional medicinal plant. Curr. Bioact. Compd. 2016; 12 (4): 297-305. [DOI:10.2174/1573407212666160426161112]
19. Pilerood SA and Prakash J. Evaluation of nutritional composition and antioxidant activity of Borage (Echium amoenum) and Valerian (Valerian officinalis). J. Food Sci. Tech. 2014; 51 (5): 845-54. [DOI:10.1007/s13197-011-0573-z]
20. Zengin G, Sarikurkcu C, Uyar P, Aktumsek A, Uysal S, Kocak MS and Ceylan R. Crepis foetida L. subsp. rhoeadifolia (Bieb.) Celak. as a source of multifunctional agents: Cytotoxic and phytochemical evaluation. J. Funct Foods 2015; 17: 698-708. [DOI:10.1016/j.jff.2015.06.041]
21. Bahadori MB, Dinparast L, Zengin G, Sarikurkcu C, Bahadori S, Asghari B and Movahhedin, N. Functional components, antidiabetic, anti-Alzheimer's disease, and antioxidant activities of Salvia syriaca L. Int. J. Food Prop. 2017; 20 (8): 1761-72. [DOI:10.1080/10942912.2016.1218893]
22. Asghari B, Mafakheri S, Zarrabi MM, Erdem, SA, Orhan IE and Bahadori MB. Therapeutic target enzymes inhibitory potential, antioxidant activity, and rosmarinic acid content of Echium amoenum. S. Afr. J. Bot. 2018; doi:10.1016/j.sajb.2018.05.017. [DOI:10.1016/j.sajb.2018.05.017]
23. Asghari B, Zengin G, Bahadori MB, Abbas-Mohammadi M and Dinparast L. Amylase, glucosidase, tyrosinase, and cholinesterases inhibitory, antioxidant effects, and GC-MS analysis of wild mint (Mentha longifolia var. calliantha) essential oil: A natural remedy. Eur. J. Integr. Med. 2018; 22: 44-9. [DOI:10.1016/j.eujim.2018.08.004]
24. Fallah Huseini H, Asghari B, Asgarpanah J, Eghbali Zarch T and Babai Zarch A. Investigation of α-Amylase and α-Glucosidases Inhibitory Effects of Silybum marianum L. Gaertn Seed Extracts in vitro. JMP. 2012; 1 (41): 239-47 (Persian).
25. Asghari B, Salehi P, Farimani MM, and Ebrahimi SN. α-Glucosidase Inhibitors from Fruits of Rosa canina L. Rec Nat Prod. 2015; 9 (3): 276-83.
26. Akkol EK, Orhan IE, and Yeşilada E. Anticholinesterase and antioxidant effects of the ethanol extract, ethanol fractions and isolated flavonoids from Cistus laurifolius L. leaves. Food Chem. 2012; 131 (2): 626-31. [DOI:10.1016/j.foodchem.2011.09.041]
27. Haddouchi F, Chaouche T, Benmansour A and Lazouni HA. Phytochemical study of Thymus fontanesii and Laurus nobilis. Der. Pharmacia. Lettre 2011; 3 (2): 343-50.
28. El-Newary SA, Shaffie NM and Omer EA. The protection of Thymus vulgaris leaves alcoholic extract against hepatotoxicity of alcohol in rats. Asian Pac. J. Trop. Med. 2017; 10 (4): 361-71. [DOI:10.1016/j.apjtm.2017.03.023]
29. Craft BD, Kerrihard AL, Amarowicz R and Pegg RB. Phenol‐based antioxidants and the in vitro methods used for their assessment. Compr. Rev. Food Sci. 2012; 11 (2): 148-73. [DOI:10.1111/j.1541-4337.2011.00173.x]
30. Medini F, Fellah H, Ksouri R and Abdelly C. Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. J. Taibah Univ. Sci. 2014; 8 (3): 216-24. [DOI:10.1016/j.jtusci.2014.01.003]
31. El-Massry KF, El-Ghorab AH, Shaaban HA and Shibamoto T. Chemical compositions and antioxidant/antimicrobial activities of various samples prepared from Schinus terebinthifolius leaves cultivated in Egypt. J. Agric. Food Chem. 2009; 57 (12): 5265-70. [DOI:10.1021/jf900638c]
32. Ertas A, Boga M, Yilmaz MA, Yesil Y, Tel G, Temel H. and Ugurlu, P. A detailed study on the chemical and biological profiles of essential oil and methanol extract of Thymus nummularius (Anzer tea): Rosmarinic acid. Ind Crops Prod. 2015; 67: 336-45. [DOI:10.1016/j.indcrop.2015.01.064]
33. Soare JR, Dinis TC, Cunha AP and Almeida, L. Antioxidant activities of some extracts of Thymus zygis. Free Radic Res. 1997; 26 (5): 469-78. [DOI:10.3109/10715769709084484]
34. Roby MHH, Sarhan MA, Selim KAH and Khalel KI. Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris L.), sage (Salvia officinalis L.), and marjoram (Origanum majorana L.) extracts. Ind. Crops Prod. 2013; 43": 827-31. [DOI:10.1016/j.indcrop.2012.08.029]
35. Fu R, Zhang Y, Guo Y, Liu F and Chen F. Determination of phenolic contents and antioxidant activities of extracts of Jatropha curcas L. seed shell, a by-product, a new source of natural antioxidant. Ind. Crops Prod. 2014; 58: 265-70. [DOI:10.1016/j.indcrop.2014.04.031]
36. do Nascimento PG, Lemos TL, Bizerra A, Arriaga Â, Ferreira DA, Santiago GM and Costa J. GM. Antibacterial and antioxidant activities of ursolic acid and derivatives. Molecules 2014; 19 (1): 1317-27. [DOI:10.3390/molecules19011317]
37. Fallah Huseini H, Asghari B, Asgarpanah J, Babai Zarch A and Eghbali Zarch T. Effect of Polar and Nom-polar Aloe vera L. Leaf extracts on α-amylase and α-Glucosidases inhibitory activity in vitro. JMP. 2013; 4 (48): 160-9 (Persian).
38. Hyun TK, Kim HC, and Kim JS. Antioxidant and antidiabetic activity of Thymus quinquecostatus Celak. Ind. Crops Prod. 2014; 52: 611-6. [DOI:10.1016/j.indcrop.2013.11.039]
39. Iauk L, Acquaviva R, Mastrojeni S, Amodeo A, Pugliese M, Ragusa M and Tundis R. Antibacterial, antioxidant and hypoglycaemic effects of Thymus capitatus (L.) Hoffmanns. Et Link leaves' fractions. J. Enzyme Inhib. Med. Chem. 2015; 30 (3): 360-5. [DOI:10.3109/14756366.2014.930453]
40. Benalla W, Bellahcen S and Bnouham M. Antidiabetic medicinal plants as a source of alpha-glucosidase inhibitors. Curr. Diabetes Rev. 2010; 6 (4): 247-54. [DOI:10.2174/157339910791658826]
41. Alzheimer's Disease International, World Alzheimer Report: The Global Prevalence of Dementia, London SE1 0BL, UK, 2009.
42. Ustun O, Senol FS, Kurkcuoglu M, Orhan I. E, Kartal M and Baser KHC. Investigation on chemical composition, anticholinesterase and antioxidant activities of extracts and essential oils of Turkish Pinus species and pycnogenol. Ind. Crops Prod. 2012; 38: 115-23. [DOI:10.1016/j.indcrop.2012.01.016]
43. Vladimir-Knežević S, Blažeković B, Kindl M, Vladić J, Lower-Nedza AD and Brantner AH. Acetylcholinesterase inhibitory, antioxidant and phytochemical properties of selected medicinal plants of the Lamiaceae family. Molecules 2014; 19 (1): 767-82. [DOI:10.3390/molecules19010767]
44. Jukic M, Politeo O, Maksimovic M, Milos M and Milos M. In vitro acetylcholinesterase inhibitory properties of thymol, carvacrol and their derivatives thymoquinone and thymohydroquinone. Phytother. Res. 2007; 21 (3): 259-61. [DOI:10.1002/ptr.2063]

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