year 5, Issue 17 (3-2006)                   J. Med. Plants 2006, 5(17): 21-26 | Back to browse issues page

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Shams Ardekani M, Hadjiakhoondi A, Jamshidi A, Mohammad Rafiee P. Pharmacognosical & Plant tissue culture studies of Achillea millefolium L.. J. Med. Plants 2006; 5 (17) :21-26
1- Faculty of pharmacy, Tehran University of Medical Sciences, Tehran, Iran ,
2- Department of Pharmacognosy, Faculty of Pharmacy and Medicinal plants Research Center, Tehran University of Medical Sciences (TUMS), Tehran, Iran
3- Institute of Medicinal Plants, ACECR, Tehran, Iran
Abstract:   (6630 Views)
Background: Achillea millefolium L. is a traditional herbal medicine used widely as a spasmolytic, anti- cancer, anti – inflammatory and carminative. This paper focuses on the analysis of the chemical composition of essential oil from the upper parts of plant and calli of Achillea millefolium L. Methods: Growing the calli of Achillea millefolium L. and production its secondary metabolites studied and compared with those in the whole plant. Achillea seeds were first surface sterilized by shaking in 3% (W/V) aqueous hydrogen peroxide, then by shaking in 5% Na hypochlorite and 80% aqueous - ethanol solution, then under aseptic condition, strile petrydishes containing autoclaved agar (0.8%) and kept in the dark at a temprature of 25-27 oC. Seedlings were developed in two weeks. Then they were transferred to strile Murashing and Skoog (MS) culture media which were containing 2,4-D (3mg/l), IAA (5mg/l) and K (0.2mg/l) as plant grow regulators. The amorph masses (calli) were produced and subcultured every 40-45 days. Results: The result obtained from GC/MS of essential oil of plant indicated that the major compounds of the essential oil are alpha – Pinene, beta – Pinene and Caryophyllene oxide. Conclusion: In contrast, calli lacked essential oil, this was indicated upon GC of its dichlorometanic extract. However phytochemical tests indicated that some tannins were produced by calli.
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Type of Study: Research | Subject: Biotechnology
Received: 2005/04/23 | Accepted: 2005/12/8 | Published: 2006/03/20

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