year 8, Issue 29 (2-2009)                   J. Med. Plants 2009, 8(29): 100-106 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghazanfari T, Yaraee R, Asoode A, Rajabian T, Kardar M, Hassan Z, et al . Purification of Immunomodulator Fraction Components of Garlic (R10) by HPLC. J. Med. Plants 2009; 8 (29) :100-106
URL: http://jmp.ir/article-1-391-en.html
1- Herbal Medicine Research Center, Shahed University, Tehran, Iran , Amiri_h_lu@yahoo.com
2- Department of Immunology, School of Medicine, Shahed University, Tehran, Iran
3- Department of Chemistry, School of Basic Sciences, Mashhad University, Tehran, Iran
4- Department of Biology, School of Basic Sciences, Shahed University, Tehran, Iran
5- Herbal Medicine Research Center, Shahed University, Tehran, Iran
6- Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
7- Department of Biochemistry, School of Basic Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:   (6699 Views)
Background: It has been reported that garlic extract and its components show medicinal effects including immunomodulatory activities. We have isolated the immunomodulatory fraction (R10) previously. Objective: In this study we have proposed to purify the components of R10 using HPLC. Methods: Crude garlic extract purchased from Hamadan, Iran. R10 fraction including 10-50 KD molecules have been isolated using ultrafiltration. Further purification has been made using Vaydac 208Tpv10, a semi-preparative HPLC reversphase choromatography column. Tricine SDS-PAGE has been used to determine molecular weight of the samples. Results: 6 major peakes were obtained from HPLC of R10 at gradient of 0.25 % of buffer B/min through 60 minute. The molecular weight of 3 peaks (samples 0, 1 and 3) was 12 KD with tricine-SDSPAGE. Conclusion: Using C8 HPLC reversphase choromatography seems to be appropriate tool for purification of R10 components and the purified components at peak 0, 1 and 3 seems to be isotypic molecules.
Full-Text [PDF 330 kb]   (2091 Downloads)    
Type of Study: Research | Subject: Pharmacognosy & Pharmaceutics
Received: 2007/09/29 | Accepted: 2008/09/4 | Published: 2009/03/18

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Medicinal Plants

Designed & Developed by : Yektaweb