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:: Volume 4, Issue 29 (3-2009) ::
JMP 2009, 4(29): 100-106 Back to browse issues page
Purification of Immunomodulator Fraction Components of Garlic (R10) by HPLC
T Ghazanfari * 1, R Yaraee 2, A Asoode 3, T Rajabian 4, M Kardar 5, ZM Hassan 6, H Naderi-Manesh 7, V Soofian 8, D Jamali 9
1- (Ph.D.) 1- Herbal Medicine Research Center, Shahed University, Tehran, Iran , Amiri_h_lu@yahoo.com
2- (Ph.D.) 2- Department of Immunology, School of Medicine, Shahed University, Tehran, Iran
3- (Ph.D.) 3- Department of Chemistry, School of Basic Sciences, Mashhad University, Tehran, Iran
4- (Ph.D.) 4- Department of Biology, School of Basic Sciences, Shahed University, Tehran, Iran
5- (M.Sc.) 1- Herbal Medicine Research Center, Shahed University, Tehran, Iran
6- (Ph.D.) 6- Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
7- (Ph.D.) 5- Department of Biochemistry, School of Basic Sciences, Tarbiat Modares University, Tehran, Iran
8- (Ph.D. student) 5- Department of Biochemistry, School of Basic Sciences, Tarbiat Modares University, Tehran, Iran
9- (B.Sc.) 2- Department of Immunology, School of Medicine, Shahed University, Tehran, Iran
Abstract:   (3464 Views)
Background: It has been reported that garlic extract and its components show medicinal effects including immunomodulatory activities. We have isolated the immunomodulatory fraction (R10) previously. Objective: In this study we have proposed to purify the components of R10 using HPLC. Methods: Crude garlic extract purchased from Hamadan, Iran. R10 fraction including 10-50 KD molecules have been isolated using ultrafiltration. Further purification has been made using Vaydac 208Tpv10, a semi-preparative HPLC reversphase choromatography column. Tricine SDS-PAGE has been used to determine molecular weight of the samples. Results: 6 major peakes were obtained from HPLC of R10 at gradient of 0.25 % of buffer B/min through 60 minute. The molecular weight of 3 peaks (samples 0, 1 and 3) was 12 KD with tricine-SDSPAGE. Conclusion: Using C8 HPLC reversphase choromatography seems to be appropriate tool for purification of R10 components and the purified components at peak 0, 1 and 3 seems to be isotypic molecules.
Keywords: Garlic, Immunomodulator fraction, HPLC, Purification
Full-Text [PDF 330 kb]   (749 Downloads)    
Type of Study: Research | Subject: Medicinal Plants
Received: 2014/11/8 | Accepted: 2014/11/8 | Published: 2014/11/8
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Ghazanfari T, Yaraee R, Asoode A, Rajabian T, Kardar M, Hassan Z, et al . Purification of Immunomodulator Fraction Components of Garlic (R10) by HPLC. JMP. 2009; 4 (29) :100-106
URL: http://jmp.ir/article-1-391-en.html


Volume 4, Issue 29 (3-2009) Back to browse issues page
فصلنامه علمی پژوهشی گیاهان دارویی Journal of Medicinal Plants
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