year 18, Issue 72 And S12 (Supplement 12 2019)
J. Med. Plants 2019, 18(72 And S12): 288-298 |
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Mirabdollahi Shamsi F, Najjari R, Moradi A, Safari F. Ameliorating of Transcription Level of gp91Phox and P22Phox Subunits of NADPH Oxdisae Complex in Hypertrophied Heart of Rats by Resveratrol. J. Med. Plants 2019; 18 (72) :288-298
URL: http://jmp.ir/article-1-2405-en.html
URL: http://jmp.ir/article-1-2405-en.html
1- Department of Physiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Department of Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3- Biotechnology Research Center, International Campus, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , f.safari@ssu.ac.ir
2- Department of Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3- Biotechnology Research Center, International Campus, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , f.safari@ssu.ac.ir
Abstract: (3432 Views)
Background: Hypertension induced-left ventricular hypertrophy (LVH) is, at least initially, an adaptive response of the heart to pressure overload but it leads to heart failure if left untreated. Over-activity of reactive oxygen species generator, NADPH oxidase enzyme, is intricately linked with LVH progression.
Objective: The aim of the present study was to investigate the effect of, natural polyphenole, resveratrol on transcription level of NADPH oxidase subunits (gp91Phox, P22Phox, P67Phox, P47Phox and Rac1) in hypertrophied heart of rats.
Method: Male Wistar rats were divided into the following groups: control (intact animal); sham (DMSO+H), untreated hypertrophy (H) and resveratrol-treated hypertrophy (R+H) groups. LVH was induced by abdominal aortic banding. Blood pressure was measured directly through carotid artery cannulation. Gene expression was evaluated using real time RT-PCR technique.
Results: The animals in H group had higher systolic (SBP) and diastolic blood pressure (DBP) compared with control (P <0.001). In treated group (R+H) SBP and DBP were decreased significantly in comparison with H group (P <0.001). In H group, cardiac mRNA levels of gp91Phox, P22Phox, P67Phox and Rac1 subunits levels were upregulated by 98.4 ± 14.5%, 64.7 ± 8.8%, 36.4 ± 5% and 73.2 ± 10.8% ,respectively (P < 0.001, P < 0.001, P < 0.05 and P < 0.001, respectively vs. control). However in R+H group gp91Phox, P22Phox and Rac1 mRNA levels were 43.2 ± 4.5%, 28.6 ± 5.7% and 30.5 ± 5.8% which showed a significant difference compared with H group (P < 0.01, P < 0.05 and P < 0.05, respectively).
Conclusion: Transcription level of NADPH oxidase subunits increases in hypertrophied heart. Resveratrol protects the heart against pressure overload-induced LVH partly through downregulation of NADPH oxidase subunits.
Objective: The aim of the present study was to investigate the effect of, natural polyphenole, resveratrol on transcription level of NADPH oxidase subunits (gp91Phox, P22Phox, P67Phox, P47Phox and Rac1) in hypertrophied heart of rats.
Method: Male Wistar rats were divided into the following groups: control (intact animal); sham (DMSO+H), untreated hypertrophy (H) and resveratrol-treated hypertrophy (R+H) groups. LVH was induced by abdominal aortic banding. Blood pressure was measured directly through carotid artery cannulation. Gene expression was evaluated using real time RT-PCR technique.
Results: The animals in H group had higher systolic (SBP) and diastolic blood pressure (DBP) compared with control (P <0.001). In treated group (R+H) SBP and DBP were decreased significantly in comparison with H group (P <0.001). In H group, cardiac mRNA levels of gp91Phox, P22Phox, P67Phox and Rac1 subunits levels were upregulated by 98.4 ± 14.5%, 64.7 ± 8.8%, 36.4 ± 5% and 73.2 ± 10.8% ,respectively (P < 0.001, P < 0.001, P < 0.05 and P < 0.001, respectively vs. control). However in R+H group gp91Phox, P22Phox and Rac1 mRNA levels were 43.2 ± 4.5%, 28.6 ± 5.7% and 30.5 ± 5.8% which showed a significant difference compared with H group (P < 0.01, P < 0.05 and P < 0.05, respectively).
Conclusion: Transcription level of NADPH oxidase subunits increases in hypertrophied heart. Resveratrol protects the heart against pressure overload-induced LVH partly through downregulation of NADPH oxidase subunits.
Keywords: gp91Phox, Left ventricular hypertrophy, NADPH oxidase, P22Phox, P67Phox, P47Phox, Rac1, Resveratrol
Type of Study: Research |
Subject:
Pharmacology & Toxicology
Received: 2018/12/27 | Accepted: 2019/03/16 | Published: 2020/03/7
Received: 2018/12/27 | Accepted: 2019/03/16 | Published: 2020/03/7
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