year 19, Issue 74 (6-2020)
J. Med. Plants 2020, 19(74): 155-162 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Yami A, Hamzeloo-Moghadam M, Karami A, Barzegar M, Amiri V, Gharehbaghian A. The apoptotic potential of ergolide to induce apoptosis in molt4 cell lines. J. Med. Plants 2020; 19 (74) :155-162
URL: http://jmp.ir/article-1-2380-en.html
URL: http://jmp.ir/article-1-2380-en.html
Amir Yami1 
, Maryam Hamzeloo-Moghadam2 
, Afshin Karami1 , Mohyedin Barzegar1 , Vahid Amiri1 , Ahmad Gharehbaghian * 3
, Maryam Hamzeloo-Moghadam2 , Afshin Karami1 , Mohyedin Barzegar1 , Vahid Amiri1 , Ahmad Gharehbaghian * 3
1- Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Pediatric Congenital Hematologic Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran ,gharehbaghian@sbmu.ac.ir
2- Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Pediatric Congenital Hematologic Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran ,
Abstract: (2664 Views)
Background: Cancer is a multi-faceted diseases caused cell proliferation in an out of control manner due to accumulation of defects and mutation in their DNA and with an impendence to invade or spread to parts of the body. During last decades new compounds with natural roots have emerged as a new paradigm for effective anti-cancer treatment. Objective: The aim of this study is to evaluate the potential of ergolide a sesquiterpene lactone with multi-functional history to induce apoptosis. Methods: Ergolide from Inula-Oculus-Christi a sesquiterpene lactone from Asteraceae plant was extracted by Traditional Medicine Research Center of Shahid Beheshti University of Medical Sciences. The cytotoxic effects of Ergolide on the acute lymphoblastic leukemia (MOLT4) and PBMC (normal cell line) were investigated at different doses for 48 hours. In this study, MTT assay and acridine orange staining were used. Also, annexin V-PI assays was utilized for further evaluation. In addition the gene expression level of BAX and BCL2 were analyzed by q-RealTime-PCR (quantitive RT-PCR). Results: The results of MTT assay demonstrated the induction of apoptosis and reduction in proliferation of MOLT4 cells treated by ergolide. (P < 0.001). Interestingly Ergolide could be less toxic in normal cells (PBMCs). AO staining and Flow cytometry analysis confirmed a significantly high percentage of autophagic and apoptotic cells compared with control groups respectively (P < 0.05). Conclusion: According to the results, ergolide demonstrated cytotoxic effects on MOLT-4, but further studies are needed to confirm its effectiveness as a complementary agent in the treatment of acute lymphoblastic leukemia.
Type of Study: Research |
Subject:
Traditional Pharmacy & Traditional Medicine
Received: 2018/09/8 | Accepted: 2018/12/23 | Published: 2020/07/21
Received: 2018/09/8 | Accepted: 2018/12/23 | Published: 2020/07/21
References
1. Terwilliger T and M. Abdul-Hay, Acute lymphoblastic leukemia: a comprehensive review and 2017 update. Blood Cancer J. 2017; 7 (6): e577. [DOI:10.1038/bcj.2017.53]
2. Millimouno FM and et al. Targeting apoptosis pathways in cancer and perspectives with natural compounds from mother nature. Cancer Prevention Res. 2014; Nov 1;7(11):1081-107 . [DOI:10.1158/1940-6207.CAPR-14-0136]
3. Coco S and et al. Identification of ALK germline mutation (3605delG) in pediatric anaplastic medulloblastoma. J. Human Genetics 2012; 57 (10): 682. [DOI:10.1038/jhg.2012.87]
4. Holohan C and et al. Cancer drug resistance: an evolving paradigm. Nature Reviews Cancer. 2013; 13 (10): 714. [DOI:10.1038/nrc3599]
5. Dall'Acqua S. Natural products as antimitotic agents. Current Topics in Medicinal Chem. 2014; 14 (20): 2272-85. [DOI:10.2174/1568026614666141130095311]
6. Borris RP. Natural products research: perspectives from a major pharmaceutical company. J. Ethnopharmacol. 1996; 51 (1 - 3): 29-38. [DOI:10.1016/0378-8741(95)01347-4]
7. Juárez P. Plant-derived anticancer agents: a promising treatment for bone metastasis. BoneKEy Reports. 2014 Dec 10;3:599. [DOI:10.1038/bonekey.2014.94]
8. Whan Han J and et al. Ergolide, sesquiterpene lactone from Inula britannica, inhibits inducible nitric oxide synthase and cyclo‐oxygenase‐2 expression in RAW 264.7 macrophages through the inactivation of NF‐κB. British J. Pharmacol. 2001; 133 (4): 503-12. [DOI:10.1038/sj.bjp.0704099]
9. Khan M and et al. Killing cancer with platycodin D through multiple mechanisms. J. Cellular and Molecular Medicine 2016; 20 (3): 389-402. [DOI:10.1111/jcmm.12749]
10. Song YJ and et al. Apoptotic potential of sesquiterpene lactone ergolide through the inhibition of NF‐κB signaling pathway. J. Pharmacy and Pharmacol. 2005; 57 (12): 1591-7. [DOI:10.1211/jpp.57.12.0009]
11. Chun JK and et al. Suppression of the NF‐kB signalling pathway by ergolide, sesquiterpene lactone, in HeLa cells. J. Pharmacy and Pharmacol. 2007; 59 (4): 561-6. [DOI:10.1211/jpp.59.4.0011]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
