year 19, Issue 73 (3-2020)                   J. Med. Plants 2020, 19(73): 152-162 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bayati R, Asadi-Gharneh H A. Study of steroidal compounds from peel and seed of some pomegranate cultivars (Punica granatum L.) and investigating the effect of pomegranate seed oil on blood lipid levels in hypercholesterolemic rabbits. J. Med. Plants. 2020; 19 (73) :152-162
URL: http://jmp.ir/article-1-2351-en.html
1- Department of Horticulture Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran , r_bayati_54@yahoo.com
2- Department of Horticulture Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran
Abstract:   (761 Views)
Background: High level of blood cholesterol can cause diseases such as atherosclerosis, high blood pressure, cardiovascular diseases, and increase the chance of apoplexy and fatty liver. Unsaturated fatty acids play important roles in preventing cardiovascular diseases because they decrease total cholesterols and LDL-C in the blood. Objective: The study investigates some sterol compounds of Iranian pomegranate seed oil and their effects on decreasing the level of cholesterol in hypercholesterolaemia rabbits. Methods: Three different varieties of pomegranate were selected and oil extraction was done from powdered tissue by Soxhlet extractor. Then, the extract was transfused into GC-MS for Identification Sterol Compounds. Oil extracted from pomegranate was taken into the diets of hypercholesterolemia rabbits. Results: The results showed that the major phytosterols in pomegranate seed and skin tissues were stigmasterols, ∆5-avenasterols, campsterols and beta-sitosterols, among which the β-sitosterols most dominant phytosterols. Studying the influence of oil extracted on decreasing cholesterol in hypercholesterolemic rabbits show that using 5 and 10 g/kg extracted oil from pomegranate seeds in the diet of hypercholesterolemic rabbits (tested with cholesterol 1%) can decrease significantly total cholesterol, triglyceride and LDL-C of hyperlipidemia against neutral sample and it can increase significantly HDL-C. Conclusion: The role of the pomegranate seed oil in improving the lipid profile of the plasma and reducing undesirable fats may be can have a considerable effect on human health and lowering blood lipids and lowering the risk of cardiovascular disease. Increasing awareness of the potential capacity of this valuable fruit can help to play a ‎better role in the various industries.
Full-Text [PDF 425 kb]   (283 Downloads)    
Type of Study: Research | Subject: Pharmacology & Toxicology
Received: 2018/11/17 | Accepted: 2019/06/15 | Published: 2020/06/6

References
1. Baharvand Ahmadi B, Eftekhari Z, Bahmani M, Jelodari M and Mirhoseini M. Overview of medicinal plants used for cardiovascular system disorders and diseases in ethnobotany of different areas in Iran. J. Herb Med Pharmacol. 2016; 5 (1): 39-44. [DOI:10.15171/jnp.2016.08]
2. Rodriguez CJ, Cai J, Swett K, González HM, Talavera GA, Wruck LM, Wassertheil‐Smoller S, Lloyd‐Jones D, Kaplan R and Daviglus ML. High cholesterol awareness, treatment, and control among Hispanic/Latinos: results from the Hispanic Community Health Study/Study of Latinos. J. the American Heart Association 2015; 24; 4 (7): e001867. [DOI:10.1161/JAHA.115.001867]
3. Trautwein EA, Duchateau GS, Lin Y, Mel'nikov SM, Molhuizen HO and Ntanios FY. Proposed mechanisms of cholesterol‐lowering action of plant sterols. European J. Lipid Science and Technol. 2003; 105 (3‐4): 171-185. [DOI:10.1002/ejlt.200390033]
4. Plat J and Mensink RP. Plant stanol esters lower serum triacylglycerol concentrations via a reduced hepatic VLDL‐1 production. Lipids 2009; 44 (12): 1149. [DOI:10.1007/s11745-009-3361-z]
5. Smet ED, Mensink RP and Plat J. Effects of plant sterols and stanols on intestinal cholesterol metabolism: suggested mechanisms from past to present. Molecular Nutrition & Food Res. 2012; 56 (7): 1058-1072. [DOI:10.1002/mnfr.201100722]
6. Kongduang D, Wungsintaweekul J and De-Eknamkul W. Biosynthesis of β-sitosterol and stigmasterol proceeds exclusively via the mevalonate pathway in cell suspension cultures of Croton stellatopilosus. Tetrahedron Letters 2008; 16: 49 (25): 4067-4072. [DOI:10.1016/j.tetlet.2008.04.049]
7. Nguyen TT. The cholesterol-lowering action of plant stanol esters. The Journal of Nutrition 1999; 1: 129 (12): 2109-2112. [DOI:10.1093/jn/129.12.2109]
8. Varasteh F, Arzani K, Zamani Z, Tabatabaei SZ. Physico-chemical seasonal changes of pomegranate (Punica granatum L.) fruit' Malas-e-Torsh-e-Saveh'in Iran. In XXVII International Horticultural Congress-IHC2006: International Symposium on Asian Plants with Unique Horticultural 769. 2006; 13: 255-258. [DOI:10.17660/ActaHortic.2008.769.36]
9. Stover ED, Mercure EW. The pomegranate: a new look at the fruit of paradise. HortScience. 2007; 1: 42 (5): 1088-1092. [DOI:10.21273/HORTSCI.42.5.1088]
10. Lansky E, Shubert S and Neeman I. Pharmacological and therapeutic properties of pomegranate. In Symposium on production, processing and marketing of pomegranate in the Mediterranean region: advances in research and technology. Séminaires Méditerranéens (CIHEAM). 2000. (pp. 231-235).
11. Bagri P, Ali M, Aeri V, Bhowmik M and Sultana S. Antidiabetic effect of Punica granatum flowers: effect on hyperlipidemia, pancreatic cells lipid peroxidation and antioxidant enzymes in experimental diabetes. Food and Chemical Toxicol. 2009; 1: 47 (1): 50-54. [DOI:10.1016/j.fct.2008.09.058]
12. Rahimi HR, Arastoo M and Ostad SN. A comprehensive review of Punica granatum (pomegranate) properties in toxicological, pharmacological, cellular and molecular biology researches. Iranian J. Pharmaceutical Res. 2012; 11 (2): 385.
13. Esmaillzadeh A, Tahbaz F, Gaieni I, Alavi-Majd H and Azadbakht L. Cholesterol-lowering effect of concentrated pomegranate juice consumption in type II diabetic patients with hyperlipidemia. International J. Vitamin and Nutrition Res. 2006; 1: 76 (3): 147-151. [DOI:10.1024/0300-9831.76.3.147]
14. Fuhrman B, Volkova N, Aviram M. Pomegranate juice inhibits oxidized LDL uptake and cholesterol biosynthesis in macrophages. The J. Nutritional Biochem. 2005; 1: 16 (9): 570-576. [DOI:10.1016/j.jnutbio.2005.02.009]
15. Zak A, Zeman M, Vitkova D, Hrabak P and Tvrzicka E. Beta-sitosterol in the treatment of hypercholesterolemia. Casopis Lekaru Ceskych. 1990; 129 (42): 1320-1323.
16. Eikani MH, Golmohammad F and Homami SS. Extraction of pomegranate (Punica granatum L.) seed oil using superheated hexane. Food and Bioproducts Processing. 2012; 1: 90 (1): 32-36. [DOI:10.1016/j.fbp.2011.01.002]
17. Matysik S, Klünemann HH and Schmitz G. Gas chromatography-tandem mass spectrometry method for the simultaneous determination of oxysterols, plant sterols, and cholesterol precursors. Clinical Chemistry 2012; 1: 58 (11): 1557-64. [DOI:10.1373/clinchem.2012.189605]
18. Amirav A, Gordin A, Poliak M and Fialkov AB. Gas chromatography‐mass spectrometry with supersonic molecular beams. Journal of Mass Spectrometry 2008; 43 (2): 141-63. [DOI:10.1002/jms.1380]
19. Lateef T and Qureshi SA. Centratherum anthelminticum and Withania coagulans improves lipid profile and oxidative stress in triton X-100 induced hyperlipidemic rabbits. Group. 2020; 1 (11.36): 1-22.
20. Pande G and Akoh CC. Antioxidant capacity and lipid characterization of six Georgia-grown pomegranate cultivars. Journal of Agricultural and Food Chemistry 2009; 10: 57 (20): 9427-9436. [DOI:10.1021/jf901880p]
21. Caligiani A, Bonzanini F, Palla G, Cirlini M and Bruni R. Characterization of a potential nutraceutical ingredient: pomegranate (Punica granatum L.) seed oil unsaponifiable fraction. Plant Foods for Human Nutrition 2010; 1: 65 (3): 277-283. [DOI:10.1007/s11130-010-0173-5]
22. Maguire LS, O'sullivan SM, Galvin K, O'connor TP and O'brien NM. Fatty acid profile, tocopherol, squalene and phytosterol content of walnuts, almonds, peanuts, hazelnuts and the macadamia nut. International J. Food Sciences and Nutrition 2004; 1: 55 (3): 171-178. [DOI:10.1080/09637480410001725175]
23. Carretero AS, Carrasco‐Pancorbo A, Cortacero S, Gori A, Cerretani L and Fernández‐Gutiérrez A. A simplified method for HPLC‐MS analysis of sterols in vegetable oil. European J. Lipid Science and Technol. 2008; 110 (12): 1142-1149. [DOI:10.1002/ejlt.200700237]
24. Laloi M, Perret AM, Chatre L, Melser S, Cantrel C, Vaultier MN, Zachowski A, Bathany K, Schmitter JM, Vallet M and Lessire R. Insights into the role of specific lipids in the formation and delivery of lipid microdomains to the plasma membrane of plant cells. Plant Physiol. 2007; 1: 143 (1): 461-472. [DOI:10.1104/pp.106.091496]
25. Vivancos M and Moreno JJ. β-Sitosterol modulates antioxidant enzyme response in RAW 264.7 macrophages. Free Radical Biology and Medicine 2005; 1: 39 (1): 91-97. [DOI:10.1016/j.freeradbiomed.2005.02.025]
26. Prieto JM, Recio MC and Giner RM. Anti-inflammatory activity of β-sitosterol in a model of oxazoloneinduced contact-delayed-type hypersensitivity. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas. 2006; 5 (3): 57-62.
27. Baskar AA, Al Numair KS, Gabriel Paulraj M, Alsaif MA, Muamar MA and Ignacimuthu S. β-sitosterol prevents lipid peroxidation and improves antioxidant status and histoarchitecture in rats with 1, 2-dimethylhydrazine-induced colon cancer. J. Medicinal Food 2012; 1: 15 (4): 335-343. [DOI:10.1089/jmf.2011.1780]
28. Aviram M. Modified forms of low density lipoprotein and atherosclerosis. Atherosclerosis 1993; 4: 98 (1): 1-9. [DOI:10.1016/0021-9150(93)90217-I]
29. Melgarejo P and Artes F. Total lipid content and fatty acid composition of oilseed from lesser-known sweet pomegranate clones. J. the Science of Food and Agriculture 2000; 80 (10): 1452-1454. https://doi.org/10.1002/1097-0010(200008)80:10<1452::AID-JSFA665>3.0.CO;2-L [DOI:10.1002/1097-0010(200008)80:103.0.CO;2-L]
30. Schubert SY, Lansky EP and Neeman I. Antioxidant and eicosanoid enzyme inhibition properties of pomegranate seed oil and fermented juice flavonoids. J. Ethnopharmacol. 1999; 1: 66 (1): 11-7. [DOI:10.1016/S0378-8741(98)00222-0]
31. Aviram M, Dornfeld L, Rosenblat M, Volkova N, Kaplan M, Coleman R, Hayek T, Presser D and Fuhrman B. Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL, and platelet aggregation: studies in humans and in atherosclerotic apolipoprotein E-deficient mice. The American J. Clinical Nutrition. 2000; 1: 71 (5): 1062-1076. [DOI:10.1093/ajcn/71.5.1062]
32. Sharifiyan F, Movahedian-Attar A, Nili N and Asgary S. Study of pomegranate (Punica granatum L.) peel extract containing anthocyanins on fatty streak formation in the renal arteries in hypercholesterolemic rabbits. Advanced Biomedical Res. 2016; 5 (8): 1-6.
33. Al-Moraie MM, Arafat RA and Al-Rasheedi AA. Effect of pomegranate juice on lipid profile and antioxidant enzymes in hypercholesterolemic rats. Life Science J. 2013; 10 (3): 2717-28.
34. Elbandy MA and Ashoush IS. Phytochemicals in pomegranate seeds and their effect as hypolipidemic agent in hypercholesterolemic rats. World J. Dairy & Food Sciences 2012; 7 (1): 85-92.
35. Rosenblat M, Hayek T and Aviram M. Anti-oxidative effects of pomegranate juice consumption by diabetic patients on serum and on macrophages. Atherosclerosis 2006; 1: 187 (2): 363-371. [DOI:10.1016/j.atherosclerosis.2005.09.006]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2021 All Rights Reserved | Journal of Medicinal Plants

Designed & Developed by : Yektaweb