year 17, Issue 65 (3-2018)
J. Med. Plants 2018, 17(65): 73-82 |
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Qavami N, Azizi M, Yazdian F, Qaderi A, Nemati H. Effective Induction of Hairy Roots in Persian Poppy (Papaver bracteatum Lindl.) Using Sonication Method. J. Med. Plants 2018; 17 (65) :73-82
URL: http://jmp.ir/article-1-2087-en.html
URL: http://jmp.ir/article-1-2087-en.html
1- Department of Horticultural Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
2- Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
3- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
2- Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
3- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
Abstract: (30996 Views)
Background: Papaver bracteatum Lindl. Commonly known as Iranian poppy is an important medicinal plant due to the presence of benzylisoquinoline alkaloids.
Objective: To evaluate the hairy root culture as a novel method for thebaine production.
Methods: To optimize the hairy root culture of P. bracteatum, five strains of Agrobacterium rhizogenes (ATCC15834, C318, A13, 9453 and A4) were used by means of Sonication-Assisted Agrobacterium-mediated Transformation (SAAT).
Results: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41°C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers.
Conclusion: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41°C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers.
Objective: To evaluate the hairy root culture as a novel method for thebaine production.
Methods: To optimize the hairy root culture of P. bracteatum, five strains of Agrobacterium rhizogenes (ATCC15834, C318, A13, 9453 and A4) were used by means of Sonication-Assisted Agrobacterium-mediated Transformation (SAAT).
Results: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41°C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers.
Conclusion: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41°C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers.
Keywords: Papaver bracteatum, Lindl. Hairy root, Sonication-assisted, Agrobacterium-mediated transformation, Thebaine
Type of Study: Research |
Subject:
Biotechnology
Received: 2017/09/10 | Accepted: 2018/01/13 | Published: 2018/03/10
Received: 2017/09/10 | Accepted: 2018/01/13 | Published: 2018/03/10
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