دوره 1، شماره 65 - ( 12-1396 )
جلد 1 شماره 65 صفحات 73-82 |
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Qavami N, Azizi M, Yazdian F, Qaderi A, Nemati H. Effective Induction of Hairy Roots in Persian Poppy (Papaver bracteatum Lindl.) Using Sonication Method
. J. Med. Plants. 2018; 1 (65) :73-82
URL: http://jmp.ir/article-1-2087-fa.html
URL: http://jmp.ir/article-1-2087-fa.html
Qavami N، Azizi M، Yazdian F، Qaderi A، Nemati H. Effective Induction of Hairy Roots in Persian Poppy (Papaver bracteatum Lindl.) Using Sonication Method
. فصلنامه گياهان دارویی. 1396; 1 (65) :73-82
1- Department of Horticultural Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
2- دانشگاه فردوسی مشهد
3- Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
4- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
2- دانشگاه فردوسی مشهد
3- Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
4- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
چکیده: (19198 مشاهده)
Background: Papaver bracteatum Lindl. Commonly known as Iranian poppy is an important medicinal plant due to the presence of benzylisoquinoline alkaloids.
Objective: To evaluate the hairy root culture as a novel method for thebaine production.
Methods: To optimize the hairy root culture of P. bracteatum, five strains of Agrobacterium rhizogenes (ATCC15834, C318, A13, 9453 and A4) were used by means of Sonication-Assisted Agrobacterium-mediated Transformation (SAAT).
Results: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41°C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers.
Conclusion: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41°C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers.
Objective: To evaluate the hairy root culture as a novel method for thebaine production.
Methods: To optimize the hairy root culture of P. bracteatum, five strains of Agrobacterium rhizogenes (ATCC15834, C318, A13, 9453 and A4) were used by means of Sonication-Assisted Agrobacterium-mediated Transformation (SAAT).
Results: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41°C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers.
Conclusion: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41°C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers.
واژههای کلیدی: Papaver bracteatum Lindl. Hairy root، Sonication-assisted Agrobacterium-mediated transformation، Thebaine
نوع مطالعه: پژوهشی |
دریافت: ۱۳۹۶/۱۲/۱۹ | پذیرش: ۱۳۹۶/۱۲/۱۹ | انتشار: ۱۳۹۶/۱۲/۱۹
دریافت: ۱۳۹۶/۱۲/۱۹ | پذیرش: ۱۳۹۶/۱۲/۱۹ | انتشار: ۱۳۹۶/۱۲/۱۹
