[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 2, Issue 42 (6-2012) ::
JMP 2012, 2(42): 98-104 Back to browse issues page
Determination of Antimicrobial Activity of Various Extracts of Stinging Nettle (Urtica dioica)
A Modarresi Chahardehi * 1, D Ibrahim 2, S Fariza Sulaiman , F Aboulhassani 3
1- 1- Industrial Biotechnology Research Laboratory, Universiti Sains Malaysia (USM), 11800 Minden , amirmch@yahoo.com
2- 1- Industrial Biotechnology Research Laboratory, Universiti Sains Malaysia (USM), 11800 Minden
3- 3- Embryology Department, School of Medical Sciences, University of Tehran
Abstract:   (3794 Views)
Background: Urtica dioica, or stinging nettle, is traditionally used in much frequency in northern Iran. A total of nine extracts were prepared using different organic solvents and by two extraction methods, either by using soxhlet extractor or sequential partitions. The antibacterial and antifungal activities of crude extracts were tested. Objective: The main purpose of this study was to determine and compare two methods of extraction and the ability of different kinds of U. dioica crude extracts to inhibit various pathogenic microorganisms. Methods: A selection of extracts that showed any activity was further tested for the minimal inhibitory concentrations (MIC) on nutrient agar and minimal bactericidal concentrations (MBC) on Muller-Hilton agar. Results: Only one extract, ethyl acetate extract of Method II (EAE II) was moderately active against Bacillus cereus, Bacillus subtilis, Vibrio parahaemyticus were 8.33, 8.33 and 0.13 mg/ml for both MIC, respectively. All others were practically inactive. Conclusion: Our finding showed that the method I had high effect on food poisoning bacterial than the method II especially against Bacillus cereus and Vibrio parahaemolytiucus.
Keywords: Stinging nettle, Antimicrobial activity, Minimum inhibitory concentration, Minimum bactericidal concentration
Full-Text [PDF 310 kb]   (1332 Downloads)    
Type of Study: Research | Subject: Biotechnology
Received: 2014/10/8 | Accepted: 2014/10/8 | Published: 2014/10/8
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Modarresi Chahardehi A, Ibrahim D, Fariza Sulaiman S, Aboulhassani F. Determination of Antimicrobial Activity of Various Extracts of Stinging Nettle (Urtica dioica). JMP. 2012; 2 (42) :98-104
URL: http://jmp.ir/article-1-165-en.html


Volume 2, Issue 42 (6-2012) Back to browse issues page
فصلنامه علمی پژوهشی گیاهان دارویی Journal of Medicinal Plants
Persian site map - English site map - Created in 0.05 seconds with 31 queries by YEKTAWEB 3790