[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 4, Number 56 (12-2015) ::
JMP 2015, 4(56): 123-138 Back to browse issues page
Quantitative and Qualitative Analysis of Anthocyanins and Flavonols in Berry Skin of some Grape Cultivars using High Performance Liquid Chromatography (HPLC)
M Pezhmanmehr1, A Ebadi *2, A Moosavi1, A R. Walker1, AR Rahimi1
1- (Ph.D.)
2- (Ph.D.) , aebadi@ut.ac.ir
Abstract:   (1647 Views)

Background: Grape berries are valuable nutraceuticals, due to the presence of different types of flavonoids as anthocyanins and flavonols, however, type and percentage of the components are influenced by genotype and environmental conditions.

Objective: To identify anthocyanins and flavonols of some important grape cultivars with different color profiles.

Methods: Berry skins of grape cultivars including Angoorab, Bidane-Ghermez, Fakhrie-Ghermez, Muskat of Hamburg, Flaim seedless, Sorkhak, and Ashe-Siahe-Shiraz were extracted using water/methanol as solvent. Anthocyanins and flavonols compositions were studied by HPLC.

Results: Highest total anthocyanin and total flavonol contents were 0.44 (Ashe-Siahe-Shiraz) and 0.67 (Angoorab) mg.100 g-1, respectively. Highest percentage of cyanidin in Bidane-Ghermez (91.6 %), peonidin in Fakhri-Ghermez (91.43 %), delphinidin in Flaim seedless (4.95 %), malvidin in Sorkhak (87.69 %) and petunidin in Muskat of Hamburg (4.36 %) were observed. Quercetin (61.21 in Ashe-Siahe-Shiraz to 87.37 % in Muskat of Hamburg) was the major flavonol, among all studied grape cultivars. Highest amounts of laricitrin in Sorkhak (16.73 %), myricetin in Ashe-Siahe-Shiraz (28.4 %), kaempferol in Flaim seedless (21.39 %) and isorhamnetin in Fakhrie-Ghermez (14.41 %) were observed. Also, estimation of hydroxylation and methylation activities showed that for both of the reactions, anthocyanins are much better substrates than flavonols.

Conclusion: The present study showed that studied grape cultivars are different for the content, composition and ratio of anthocyanins and flavonols and pattern of hydroxylation and methylation, which could lead to differences in their nutraceutical values specially their antioxidant activity, stability, solubility and color properties.

Keywords: Vitis vinifera, Anthocyanins, Flavonols, HPLC
Full-Text [PDF 1256 kb]   (802 Downloads)    
Type of Study: Research | Subject: gardening
Received: 2016/02/14 | Accepted: 2016/02/14 | Published: 2016/02/14
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Pezhmanmehr M, Ebadi A, Moosavi A, R. Walker A, Rahimi A. Quantitative and Qualitative Analysis of Anthocyanins and Flavonols in Berry Skin of some Grape Cultivars using High Performance Liquid Chromatography (HPLC). JMP. 2015; 4 (56) :123-138
URL: http://jmp.ir/article-1-1232-en.html
Volume 4, Number 56 (12-2015) Back to browse issues page
فصلنامه علمی پژوهشی گیاهان دارویی Journal of Medicinal Plants
Persian site map - English site map - Created in 0.048 seconds with 803 queries by yektaweb 3506