سال 9، شماره 33 و S6 - ( supplement 6 1388 )                   سال 9 شماره 33 و S6 صفحات 65-57 | برگشت به فهرست نسخه ها

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Teimori M, Montasser Kouhsari S, Ghafarzadegan R, Hajiaghaee R. Study of Hypoglycemic Effect of Juglans regia Leaves and its Mechanism. J. Med. Plants 2010; 9 (33) :57-65
URL: http://jmp.ir/article-1-514-fa.html
تیموری مریم، منتسر کوهساری ، غفارزادگان رضا، حاجی آقایی رضا. Study of Hypoglycemic Effect of Juglans regia Leaves and its Mechanism. فصلنامه گياهان دارویی 1388; 9 (33) :65-57

URL: http://jmp.ir/article-1-514-fa.html


1- Department of Cellular and Molecular Biology, School of Biology, Faculty of Science, University of Tehran
2- Department of Pharmacognosy and Pharmaceutics, Institute of Medicinal plants, ACECR, Karaj ، reza.ghafary@yahoo.com
3- Department of Pharmacognosy and Pharmaceutics, Institute of Medicinal plants, ACECR, Karaj
چکیده:   (4181 مشاهده)

Background: Hypoglycemic effect of Juglans regia leaves was reported in other previous researches.

Objective: To investigate the mechanism of hypoglycemic action of Julans regia Leaves Methanolic Extract (JRLME), we designed the following study.

Methods: Male wistar rats were classified in five groups, each of six, including alloxan-induced diabetic rats treated with JRLME at doses of 250 (D+JRLMEa), 500 mg/kg (D+JRLMEb), Acarbose at dose 20 mg/kg (D+Ac), vehicle alone (DC) and normal rats treated with vehicle alone (NC). The Postprandial blood glucose level was examined in a short time (after a single dose treatment) and a long time (after three weeks daily treatment) model. After three weeks of treatment, all groups’ animals were killed to dissect pancreatic and myocardium for RNA extraction and RT-PCR, to assess insulin and glut-4 genes expression, respectively. In addition, other normal rats were killed to extract intestinal brush border membrane for α-glucosidase solution preparation. The effect of JRLMEa on α-glucosidase activity was evaluated by an in vitro method, compared to Acarbose as a reference drug.

Results: The plant extract had a significant hypoglycemic action in both short and long term models. Decrease in PBG level was the most at 8 hour after treatment and more in D+Ac (53%), D+JRLMEa (40%) and D+JRLMEb (29%) versus DC, respectively. There was also permanent PBG reduction in treated groups in comparison with the DC one during long term period.

Conclusion: We did not find change in the insulin and glut-4 genes expression. On the other hand, the in vitro assay of α-glucosidase activity displayed inhibitory action of JRLME, like Acarbose, but less effectively.
واژه‌های کلیدی: <، i>، Juglans regia<، /i>، Blood Glucose، insulin، glut-4، α-Glucosidase
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نوع مطالعه: پژوهشی | موضوع مقاله: فارماكوگنوزی و فارماسيوتيكس
دریافت: 1388/8/6 | پذیرش: 1388/12/10 | انتشار: 1388/12/28

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