year 6, Issue 22 (5-2007)                   J. Med. Plants 2007, 6(22): 64-70 | Back to browse issues page

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Behravan J, Mosaffa F, Karimi G, Iranshahi M. In vitro Protective Effects of Satureja hortensis L. Essential Oil and Ethanolic Extract on Lymphocytes DNA. J. Med. Plants. 2007; 6 (22) :64-70
URL: http://jmp.ir/article-1-617-en.html
1- 1- Department of Pharmacognosy and Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran , behravanj@yahoo.com
2- 2- Biotechnology Laboratory, Biotechnology and Pharmaceutical Sciences Research Centers, Mashhad University of Medical Sciences, Mashhad, Iran
3- 3- Department of Pharmacodinamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract:   (3560 Views)
Background: DNA damage and oxidative stress are widely recognized as major factors in many degenerative diseases and aging. Objectives: The protective properties of Satureja hortensis L. on the rat lymphocytes DNA lesions were tested. Method: Lymphocytes were isolated from blood samples taken from healthy rats. DNA breaks and resistance to H2O2-induced damage were measured with the comet assay. Rat lymphocytes were incubated in S. hortensis ethanolic extract (SHE) (0.05, 0.1, 0.5, 1 and 2.5 mg/ml), essential oil (SHEO) (0.05, 0.1, 0.5, 1 and 2.5 µl/ml), H2O2 (50, 100 and 200 µM), a combination of H2O2 (200 M) with either SHE (1, 2.5 mg/ml) or SHEO (1, 2.5 µl/ml) at 4oC for 30 minutes. The extent of DNA migration was measured using a single-cell microgel electrophoresis technique under alkaline conditions. Results: Treatment of rat lymphocytes with SHE or SHEO resulted in significant reduction of H2O2-induced DNA damage compared to controls. SHE exhibited a significant (p<0.01) inhibitory effect on oxidative DNA damage at 2.5 mg/ml. SHEO (1 and 2.5 µl/ml) also showed significant inhibitory effects (p <0.01) on H2O2 induced chromosomal damage. Conclusion: Both the ethanolic extract and the essential oil of the plant were able to reverse the oxidative damage on rat lymphocytes induced by hydrogen peroxide.
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Type of Study: Research | Subject: Pharmacognosy & Pharmaceutics
Received: 2014/11/30 | Accepted: 2014/11/30 | Published: 2014/11/30

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