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:: Volume 2, Issue 70 (7-2019) ::
J. Med. Plants 2019, 2(70): 173-180 Back to browse issues page
Optimization of Thebaine Production using Adventitious Roots of Papaver bracteatum Lindle. by Alteration of Aeration Volume and Temperature in Bioreactor
N Qavami *1, H Naghdi Badi2, A Qaderi2, A Mehrafarin2, F Khalighi-Sigaroodi2, AR Zare karizi2
1- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran , nassrinqavami@gmail.com
2- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
Abstract:   (320 Views)
Background: Nowadays, the optimization of the culture conditions in bioreactors is considered as an approach to produce secondary metabolites such as thebaine.
Thebaine is the dominant alkaloid in Iranian poppy that is used as a precursor for the synthesis of analgesic compounds.
Objective: Optimization of culture conditions of Papaver bracteatum adventitious roots in bioreactor for the scale-up thebaine production.
Methods: In this study, adventitious roots was induced from the stem explants and then cultured in a bubble column bioreactor. The research was conducted as a factorial experiment based on randomized complete design. The bioreactor temperatures were 14, 20, 26 and 32 °C, as well as aeration volumes were 0.05, 0.1, 0.2 and 0.4 vvm.
Results: The results showed that the temperature and aeration volume had significant effect on root fresh weight and thebaine content. The highest root dry weight was related to aeration volume of 0.2 vvm and temperature of 26°C. The maximum content of thebaine was observed in aeration volume of 0.2 vvm and temperature of 26°C.
Conclusion: According to the results, aeration volume and temperature were two important factors for large-scale production of Papaver bracteatum biomass and thebaine in bioreactor conditions.
Keywords: Papaver bracteatum, Adventirious root, Bioreactor, Thebaine
Full-Text [PDF 434 kb]   (118 Downloads)    
Type of Study: Research | Subject: Medicinal Plants
Received: 2019/06/26 | Accepted: 2019/06/26 | Published: 2019/06/26
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Qavami N, Naghdi Badi H, Qaderi A, Mehrafarin A, Khalighi-Sigaroodi F, Zare karizi A. Optimization of Thebaine Production using Adventitious Roots of Papaver bracteatum Lindle. by Alteration of Aeration Volume and Temperature in Bioreactor. J. Med. Plants. 2019; 2 (70) :173-180
URL: http://jmp.ir/article-1-2572-en.html


Volume 2, Issue 70 (7-2019) Back to browse issues page
فصلنامه علمی پژوهشی گیاهان دارویی Journal of Medicinal Plants
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