year 18, Issue 70 (5-2019)                   J. Med. Plants 2019, 18(70): 173-180 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Qavami N, Naghdi Badi H, Qaderi A, Mehrafarin A, Khalighi-Sigaroodi F, Zare karizi A. Optimization of Thebaine Production using Adventitious Roots of Papaver bracteatum Lindle. by Alteration of Aeration Volume and Temperature in Bioreactor. J. Med. Plants. 2019; 18 (70) :173-180
URL: http://jmp.ir/article-1-2572-en.html
1- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran , nassrinqavami@gmail.com
2- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran, Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
Abstract:   (865 Views)
Background: Nowadays, the optimization of the culture conditions in bioreactors is considered as an approach to produce secondary metabolites such as thebaine.
Thebaine is the dominant alkaloid in Iranian poppy that is used as a precursor for the synthesis of analgesic compounds.
Objective: Optimization of culture conditions of Papaver bracteatum adventitious roots in bioreactor for the scale-up thebaine production.
Methods: In this study, adventitious roots was induced from the stem explants and then cultured in a bubble column bioreactor. The research was conducted as a factorial experiment based on randomized complete design. The bioreactor temperatures were 14, 20, 26 and 32 °C, as well as aeration volumes were 0.05, 0.1, 0.2 and 0.4 vvm.
Results: The results showed that the temperature and aeration volume had significant effect on root fresh weight and thebaine content. The highest root dry weight was related to aeration volume of 0.2 vvm and temperature of 26°C. The maximum content of thebaine was observed in aeration volume of 0.2 vvm and temperature of 26°C.
Conclusion: According to the results, aeration volume and temperature were two important factors for large-scale production of Papaver bracteatum biomass and thebaine in bioreactor conditions.
Full-Text [PDF 689 kb]   (315 Downloads)    
Type of Study: Research | Subject: Biotechnology
Received: 2018/11/5 | Accepted: 2019/01/21 | Published: 2019/05/26
* Corresponding Author Address: Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran

References
1. Mozaffarian V. A Dictionary of Iranian Plant Names 2012, Iran: Farhang Moaser Press.
2. Fairbairn J W and Hakim F. Papaver bracteatum Lindl.-a new plant source of opiates. J. Pharm. Pharmacol. 1973; 25: 353-8. [DOI:10.1111/j.2042-7158.1973.tb10028.x]
3. Nyman U and Bruhn J G. Papaver bracteatum Lindl.-a summary of current knowledge. Planta Medica. 1979; 35: 98-117. [DOI:10.1055/s-0028-1097192]
4. Kapoor L D. Opium Poppy: botany, chemistry and pharmacology. 1997, USA: Food Products Press.
5. Kettenes-van de Bosch J, Salemink J C A and Khan I. Biological activity of the alkaloids of Papaver bracteatum Lind. Journal of Ethnopharmacology 1981; 3 (1): 21-38. [DOI:10.1016/0378-8741(81)90012-X]
6. Palevitch D and Levy A. Domestication of Papaver bracteatum as a source of thebaine. Acta Horticulturae. 1992; 306: 33-52. [DOI:10.17660/ActaHortic.1992.306.2]
7. Murthy H N and Praveen N. Carbon sources and medium pH affects the growth of Withania somnifera (L.) Dunal adventitious roots and withanolide A production. Natural Product Res. 2008; 27 (2): 185-9. [DOI:10.1080/14786419.2012.660691]
8. Ramirez-Carvajal G A, Morse A M, Dervinis C and Davis J M. The cytokinin type-B response regulator PtRR13 is a negative regulator of adventitious root development in Populus. Plant Physiol. 2009; 150 (2): 759-771. [DOI:10.1104/pp.109.137505]
9. Deepthi S and Satheeshkumar K. Effects of major nutrients, growth regulators and inoculum size on enhanced growth and camptothecin production in adventitious root cultures of Ophiorrhiza mungos L. Biochemical Engineering J. 2017; 117: 198-209. [DOI:10.1016/j.bej.2016.10.016]
10. Chaterjeea A, Shuklaa S, Mishrab P, Rastogia A and Singh S. Prospects of in vitro production of thebaine in opium poppy (Papaver somniferum L.). Industrial Crops and Products 2010; 32: 668-70. [DOI:10.1016/j.indcrop.2010.04.007]
11. Yesil-Celiktas O, Gurel A and Vardar-Sukan F, Large scale cultivation of plant cell and tissue culture in bioreactors. 2010, Kerala: Transworld Research Network.
12. Jeong C S, Murthy H N, Hahn E J, Lee H L and Paek K Y. Inoculum size and auxin concentration influence the growth of adventitious roots and accumulation of ginsenosides in suspension cultures of Panax ginseng (C. A. Meyer). Acta Physiologiae Plantarum. 2009; 31; 09; 219-22. [DOI:10.1007/s11738-008-0206-y]
13. Paek K, Murthy H, Hahn E and Zhong J. Large scale culture of ginseng adventitious roots for production of ginsenosides. Advanced Biochemical Engineering Biotechnol. 2009; 113: 151-76. [DOI:10.1007/10_2008_31]
14. Ballica R and Ryu D. Effects of rheological properties and mass transfer on plant cell bioreactor performance: production of tropane alkaloids. Biotechnology Bioengineering 1993; 42: 1181-89. [DOI:10.1002/bit.260421008]
15. Wu S, Yu X, Lian M, Park S and Piao X. Several factors affecting hypericin production of Hypericum perforatum during adventitious root culture in airlift bioreactors. Acta Physiologiae Plantarum. 2014; 36: 975-81. [DOI:10.1007/s11738-013-1476-6]
16. Lee J, Seong E, Goh E, NY K and Yu C. Factors involved in mass propagation of Ginseng (Panax ginseng C. A. Meyer) using bioreactor system. J. Korean Society Applied Biological Chemistry 2009; 52: 466 - 71. [DOI:10.3839/jksabc.2009.080]
17. McClung C R. Circadian rhythms in plants. Plant Physiol. 2001; 52: 139 - 62. [DOI:10.1146/annurev.arplant.52.1.139]
18. Luschnig C. Auxin transport: ABC proteins join the club. Trends Plant Science 2002; 7 (8): 329 - 32 [DOI:10.1016/S1360-1385(02)02292-6]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2020 All Rights Reserved | Journal of Medicinal Plants

Designed & Developed by : Yektaweb