[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 4, Number 52 (12-2014) ::
JMP 2014, 4(52): 154-162 Back to browse issues page
Genetic and Phytochemical Evaluation of Tissue Culture-derived Plants in Fennel (Foeniculum vulgar) under Different Concentrations of Growth Regulators
L Shooshtari1, A Etminan1, A Mehrafarin2, A Qaderi *3
1- (Ph.D.) Department of Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
2- (Ph.D.) Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
3- (Ph.D.) Biotechnology Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran , Ardeshir582003@yahoo.com
Abstract:   (3659 Views)
Background: The genetic diversity among plants derived from tissue culture is called somaclonal variation, which provides a valuable source of genetic variation for the improvement of medicinal plants. Objective: The present study was conducted to investigate the efficiency of molecular markers in detection of somaclonal variation and to assess the importance of DNA methylation in occurrence of genomic changes. Methods: The genomic DNA of a normal plant and eight abnormal regenerated plants from calluses cultured in different conditions were extracted using modified Delaporta method. The AFLP procedure was performed with application of two different double digestion methods using restriction enzymes. The digested fragments were ligated to appropriate adaptors and amplification was carried out using appropriate primers. Also percentage and component of essential oil were indicated by GC/MS analysis. Results: Analysis of banding patterns showed high differences in amount of polymorphism detected between two different double digestion methods. According to the results of cluster analysis based on the Jaccard's similarity coefficient, all tested plants divided into two main group. While the first group contained only normal sample, other abnormal samples were placed in the second group. Phytochemical analysis showed that the important secondary metabolites such as Limonene, Fenchone, Estragole ,Anethole didn’t produce in invitro culture condition. In contrast some metabolites like Cineol, Terpineol, 2,4 Decadienal produce just in invitro culture. Conclusion: The results indicated that the used method has the potential to be used for assessment of somaclonal variations in regenerated plants. Additionally, considering characters of served enzymes in this study, phenotypic variations in abnormal plants that are resulted from somaclonal variation can be related to genome methylation.
Keywords: Foeniculum vulgar, Methylation, Somaclonal variation, Tissue culture
Full-Text [PDF 361 kb]   (1502 Downloads)    
Type of Study: Research | Subject: Biotechnology
Received: 2015/01/31 | Accepted: 2015/01/31 | Published: 2015/01/31
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shooshtari L, Etminan A, Mehrafarin A, Qaderi A. Genetic and Phytochemical Evaluation of Tissue Culture-derived Plants in Fennel (Foeniculum vulgar) under Different Concentrations of Growth Regulators. JMP. 2014; 4 (52) :154-162
URL: http://jmp.ir/article-1-881-en.html
Volume 4, Number 52 (12-2014) Back to browse issues page
فصلنامه علمی پژوهشی گیاهان دارویی Journal of Medicinal Plants
Persian site map - English site map - Created in 0.048 seconds with 794 queries by yektaweb 3461